Human Phospho-Ubiquitin (S65) Antibody

R&D Systems | Catalog # MAB9866

Recombinant Monoclonal Antibody.
R&D Systems
Discontinued Product
MAB9866 has been discontinued. View all Ubiquitin products.

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1272B
View all Ubiquitin Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide containing human Phospho-Ubiquitin (S65)
Accession # P0CG47

Specificity

Detects Ubiquitin when phosphorylated at Serine 65.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human Phospho-Ubiquitin (S65) Antibody

Detection of Phospho-Ubiquitin (S65) antibody by Western Blot.

Detection of Phospho-Ubiquitin (S65) by Western Blot.

Western blot shows lysates of Di-Ubiquitin (negative control), Phospho-Tetra-Ubiquitin (S65), Phospho-Ubiquitin (S65), K48 linked Phosopho-Tetra-Ubiquitin (S65), and K48 linked Ubiquitin 2-7 (negative control). PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human Phospho-Ubiquitin (S65) Monoclonal Antibody (Catalog # MAB9866, left panel) or 0.1 µg/mL of Rabbit Anti-Human Ubiquitin Monoclonal Antibody (Catalog # MAB8595, right panel), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-Ubiquitin (S65) at approximately 10, 30, and 35 kDa (as indicated on the left panel). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Phospho-Ubiquitin (S65) Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: Phospho-Tetra-Ubiquitin (S65), Phospho-Ubiquitin (S65), and K48 linked Phospho-Tetra-Ubiquitin (S65)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Ubiquitin

Ubiquitin is a 76 amino acid, highly conserved nuclear and cytoplasmic protein. It is found exclusively in eukaryotes, becomes covalently attached to substrate proteins by enzymes in the Ubiquitin-Proteosome Pathway (UPP) and has a major role in targeting cellular proteins for the ATP-dependent degradation by the 26S proteosome. Ubiquitination also affects proteosome-independent events such as protein localization, activity and function.

Alternate Names

UBB

Entrez Gene IDs

7314 (Human); 298693 (Rat)

Gene Symbol

UBB

UniProt

P0CG47

Additional Ubiquitin Products

Product Documents for Human Phospho-Ubiquitin (S65) Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Phospho-Ubiquitin (S65) Antibody

For research use only

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