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Human Phospho-Ubiquitin (S65) Antibody

Catalog #: MAB9866 Datasheet / COA / SDS
Recombinant Monoclonal Antibody

Discontinued Product

MAB9866 has been discontinued.
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Detection of Phospho-Ubiquitin (S65) by Western Blot.
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Human Phospho-Ubiquitin (S65) Antibody Summary

Species Reactivity
Human
Specificity
Detects Ubiquitin when phosphorylated at Serine 65.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1272B
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Synthetic peptide containing human Phospho-Ubiquitin (S65)
Accession # P0CG47
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Phospho-Ubiquitin (S65) antibody by Western Blot. View Larger

Detection of Phospho-Ubiquitin (S65) by Western Blot. Western blot shows lysates of Di-Ubiquitin (negative control), Phospho-Tetra-Ubiquitin (S65), Phospho-Ubiquitin (S65), K48 linked Phosopho-Tetra-Ubiquitin (S65), and K48 linked Ubiquitin 2-7 (negative control). PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human Phospho-Ubiquitin (S65) Monoclonal Antibody (Catalog # MAB9866, left panel) or 0.1 µg/mL of Rabbit Anti-Human Ubiquitin Monoclonal Antibody (Catalog # MAB8595, right panel), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-Ubiquitin (S65) at approximately 10, 30, and 35 kDa (as indicated on the left panel). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ubiquitin

Ubiquitin is a 76 amino acid, highly conserved nuclear and cytoplasmic protein. It is found exclusively in eukaryotes, becomes covalently attached to substrate proteins by enzymes in the Ubiquitin-Proteosome Pathway (UPP) and has a major role in targeting cellular proteins for the ATP-dependent degradation by the 26S proteosome. Ubiquitination also affects proteosome-independent events such as protein localization, activity and function.

Entrez Gene IDs
7314 (Human); 298693 (Rat)
Alternate Names
RPS27A; UBA52; UBB ubiquitin B; UBB; UBC; Ubiquitin

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