Human Plexin A1 Alexa Fluor® 647-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB65361R-025
Detection of Plexin A1 in Human PBMCs by Flow Cytometry.
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Human Plexin A1 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Plexin A1 in direct ELISA.
Source
Monoclonal Mouse IgG2B Clone # 708954
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Plexin A1
Tyr964-Leu1152
Accession # Q9UIW2
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647

Applications

Recommended Concentration
Sample
Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of Plexin A1 in Human PBMCs by Flow Cytometry. View Larger

Detection of Plexin A1 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cell (PBMCs) were stained with Mouse Anti-Human Plexin A1 Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # FAB65361R, filled histogram) or isotype control antibody (Catalog # IC0041R, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of Plexin A1 in K562 Human Cell Line by Flow Cytometry. View Larger

Detection of Plexin A1 in K562 Human Cell Line by Flow Cytometry. K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human Plexin A1 Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # FAB65361R, filled histogram) or isotype control antibody (Catalog # IC0041R, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Plexin A1

Plexin A1, also known as NOV, is an approximately 250 kDa type I transmembrane glycoprotein member of the Plexin family of Semaphorin signal transducers. It consists of a 1218 amino acid (aa) extracellular region with one Sema domain, a spacer, and four tandem IPT/TIG domains, a 21 aa transmembrane segment, and a 631 aa cytoplasmic domain. Plexin A1 binds the transmembrane Semaphorin 6D, directly and binds the Class 3 secreted Semaphorins indirectly via Neuropilin-1 and -2. It mediates cytoskeletal remodeling that results in cell migration and neuronal axon repulsion. Within aa 964-1152 (in the IPT/TIG region), human Plexin A1 shares 93% aa sequence identity with mouse and rat Plexin A1.

Entrez Gene IDs
5361 (Human); 18844 (Mouse)
Alternate Names
NOV; NOVP; Plexin A1; PLEXIN-A1; PLXN1; PLXNA1

Product Datasheets

FAQs

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