Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Sheep IgG
View all Plexin B2 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Plexin B2

Specificity

Detects human Plexin B2 in Western blots. In Western blots, less than 1% cross‑reactivity with recombinant human (rh) Plexin A4, rhPlexin B1, rhPlexin B3, rhPlexin C1, and rhPlexin D1 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Human Plexin B2 Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human Plexin B2

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Plexin B2

Plexin B2 is a 240 kDa member of the B subfamily, Plexin family of semaphorin receptors. It is expressed by cerebellar granule cells, neuroepithelium and chondrocytes and serves as a receptor for Sema4C. Mature human Plexin B2 is 1819 amino acid (aa) in length. It is a type I transmembrane (TM) glycoprotein that contains an 1178 aa extracellular domain (ECD) plus a 620 aa cytoplasmic region. The ECD contains one semaphorin domain (aa 20‑466) and three IPT repeats (aa 803‑1092). The ECD may be cleaved into two subunits, a 170 kDa alpha ‑chain (aa 20‑1164) and an 80 kDa TM beta -chain that remain noncovalently-linked. Multiple splice variants may exist. One shows an alternate start site at Met1369, while a second shows a deletion of aa 67‑638. Over aa 20‑1160, human Plexin B2 is 82% aa identical to mouse Plexin B2.

Alternate Names

MM1, PLEXB2, PLXNB2

Entrez Gene IDs

23654 (Human); 140570 (Mouse); 315217 (Rat)

Gene Symbol

PLXNB2

Additional Plexin B2 Products

Product Documents for Human Plexin B2 Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Plexin B2 Biotinylated Antibody

For research use only

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