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Streptavidin-HRP

DY998 61 Citations 12 Reviews Datasheet / COA / SDS
Set of 5 vials enough for 75 plates total
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DY998
Streptavidin-HRP
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Citations (61)
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Reviews (12)
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Streptavidin-HRP Summary

INTENDED USE & DESCRIPTION

Streptavidin-HRP (Part # 890803) contains 5 vials (1 mL/vial) of streptavidin conjugated to horseradish peroxidase for use with biotinylated detection antibodies in ELISAs and Western Blotting.

STORAGE & STABILITY

Store unopened at 2-8 °C. Stable for up to 6 months after opening when stored at 2-8 °C. Do Not Freeze.

REAGENT PREPARATION

For use in ELISAs, dilute to the working concentration specified on the vial label using 1% BSA/PBS or Reagent Diluent Concentrate 2 (Catalog # DY995) diluted 1:10 in deionized water. For use in Western blots, the suggested dilution is 1:200-1:2000 in an appropriate diluent. Use the diluted reagent within 60 minutes. 

Specifications

Shipping Conditions
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Applications
ELISA

Product Datasheets

Product Datasheet
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Streptavidin-HRP

Reagents Provided

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Citations for Streptavidin-HRP

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

61 Citations: Showing 1 - 10
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  1. Targeting VLA4 integrin and CXCR2 mobilizes serially repopulating hematopoietic stem cells
    Authors: D Karpova, MP Rettig, J Ritchey, D Cancilla, S Christ, L Gehrs, E Chendamara, MO Evbuomwan, M Holt, J Zhang, G Abou-Ezzi, H Celik, E Wiercinska, W Yang, F Gao, LG Eissenberg, RF Heier, SD Arnett, MJ Meyers, MJ Prinsen, DW Griggs, A Trumpp, PG Ruminski, DM Morrow, HB Bonig, DC Link, JF DiPersio
    J. Clin. Invest., 2019;129(7):2745-2759.  2019
  2. A ribose-functionalized NAD+ with unexpected high activity and selectivity for protein poly-ADP-ribosylation
    Authors: XN Zhang, Q Cheng, J Chen, AT Lam, Y Lu, Z Dai, H Pei, NM Evdokimov, SG Louie, Y Zhang
    Nat Commun, 2019;10(1):4196.  2019
  3. Engineering of CHO cells for the production of vertebrate recombinant sialyltransferases
    Authors: B Houeix, MT Cairns
    PeerJ, 2019;7(0):e5788.  2019
  4. Dengue virus nonstructural protein 1 activates platelets via Toll-like receptor 4, leading to thrombocytopenia and hemorrhage
    Authors: CH Chao, WC Wu, YC Lai, PJ Tsai, GC Perng, YS Lin, TM Yeh
    PLoS Pathog., 2019;15(4):e1007625.  2019
  5. Cecropia obtusa extract and chlorogenic acid exhibit anti aging effect in human fibroblasts and keratinocytes cells exposed to UV radiation
    Authors: GAD Alves, R Oliveira d, HL Ghislain R, H Masaki, MJV Fonseca
    PLoS ONE, 2019;14(5):e0216501.  2019
  6. Correction of bleeding in experimental severe hemophilia A by systemic delivery of factor VIII-encoding mRNA
    Authors: J Russick, S Delignat, P Milanov, O Christophe, G Boros, CV Denis, PJ Lenting, SV Kaveri, S Lacroix-De
    Haematologica, 2019;0(0):.  2019
  7. Cell-Free DNA and CXCL10 Derived from Bronchoalveolar Lavage Predict Lung Transplant Survival
    Authors: JYC Yang, SE Verleden, A Zarinsefat, BM Vanaudenae, R Vos, GM Verleden, RD Sarwal, TK Sigdel, JM Liberto, I Damm, D Watson, MM Sarwal
    J Clin Med, 2019;8(2):.  2019
  8. Estrogen induces St6gal1 expression and increases IgG sialylation in mice and patients with rheumatoid arthritis: a potential explanation for the increased risk of rheumatoid arthritis in postmenopausal women
    Authors: C Engdahl, A Bondt, U Harre, J Raufer, R Pfeifle, A Camponesch, M Wuhrer, M Seeling, IL Mårtensson, F Nimmerjahn, G Krönke, HU Scherer, H Forsblad-d, G Schett
    Arthritis Res. Ther., 2018;20(1):84.  2018
  9. A CpG-adjuvanted intranasal enterovirus 71 vaccine elicits mucosal and systemic immune responses and protects human SCARB2-transgenic mice against lethal challenge
    Authors: YL Lin, YH Chow, LM Huang, SM Hsieh, PY Cheng, KC Hu, BL Chiang
    Sci Rep, 2018;8(1):10713.  2018
  10. Structure of the 4-1BB/4-1BBL complex and distinct binding and functional properties of utomilumab and urelumab
    Authors: SM Chin, CR Kimberlin, Z Roe-Zurz, P Zhang, A Xu, S Liao-Chan, D Sen, AR Nager, NS Oakdale, C Brown, F Wang, Y Yang, K Lindquist, YA Yeung, S Salek-Arda, J Chaparro-R
    Nat Commun, 2018;9(1):4679.  2018
  11. Impact of immunization against OxLDL on the pulmonary response to cigarette smoke exposure in mice
    Authors: M Talbot, M Hamel-Auge, MJ Beaulieu, M Gazzola, A Lechasseur, S Aubin, MÈ Paré, D Marsolais, Y Bossé, MC Morissette
    Respir. Res., 2018;19(1):131.  2018
  12. A systematic analysis of VRC01 antibody expression in Pichia pastoris through signal peptide optimization
    Authors: R Aw, PF McKay, RJ Shattock, KM Polizzi
    Protein Expr. Purif., 2018;0(0):.  2018
  13. Exploring Serum Levels of Brain Derived Neurotrophic Factor and Nerve Growth Factor Across Glaucoma Stages
    Authors: F Oddone, G Roberti, A Micera, A Busanello, S Bonini, L Quaranta, L Agnifili, G Manni
    PLoS ONE, 2017;12(1):e0168565.  2017
  14. Hydrogels with precisely controlled integrin activation dictate vascular patterning and�permeability
    Authors: S Li, LR Nih, H Bachman, P Fei, Y Li, E Nam, R Dimatteo, ST Carmichael, TH Barker, T Segura
    Nat Mater, 2017;16(9):953-961.  2017
  15. Inhibition of DNA replication by an anti-PCNA aptamer/PCNA complex
    Authors: E Kowalska, F Bartnicki, R Fujisawa, P Bonarek, P Hermanowic, T Tsurimoto, K Muszynska, W Strzalka
    Nucleic Acids Res., 2017;0(0):.  2017
  16. Immunization with Live Human Rhinovirus (HRV) 16 Induces Protection in Cotton Rats against HRV14 Infection
    Authors: MC Patel, LM Pletneva, MS Boukhvalov, SN Vogel, AE Kajon, JCG Blanco
    Front Microbiol, 2017;8(0):1646.  2017
  17. A novel recombinant antibody specific to full-length stromal derived factor-1 for potential application in biomarker studies
    Authors: DI Bromage, S Taferner, M Pillai, DM Yellon, SM Davidson
    PLoS ONE, 2017;12(4):e0174447.  2017
  18. Inhibitory Effects of Polyacetylene Compounds from Panax ginseng on Neurotrophin Receptor-Mediated Hair Growth
    Authors: A Suzuki, D Matsuura, H Kanatani, S Yano, M Tsunakawa, S Matsuyama, H Shigemori
    Biol. Pharm. Bull., 2017;40(10):1784-1788.  2017
  19. Development of a Glycosaminoglycan Derived, Selectin Targeting Anti-Adhesive Coating to Treat Endothelial Cell Dysfunction
    Authors: JR Wodicka, AM Chambers, GS Sangha, CJ Goergen, A Panitch
    Pharmaceuticals (Basel), 2017;10(2):.  2017
  20. Vitronectin as a Micromanager of Cell Response in Material-Driven Fibronectin Nanonetworks
    Authors: M Cantini, K Gomide, V Moulisova, C González-G, M Salmerón-S
    Adv Biosyst, 2017;1(9):1700047.  2017
  21. TLR4 and TLR7/8 Adjuvant Combinations Generate Different Vaccine Antigen-Specific Immune Outcomes in Minipigs when Administered via the ID or IN Routes.
    Authors: McKay P, King D, Mann J, Barinaga G, Carter D, Shattock R
    PLoS ONE, 2016;11(2):e0148984.  2016
  22. Impact of LbSapSal Vaccine in Canine Immunological and Parasitological Features before and after Leishmania chagasi-Challenge
    PLoS ONE, 2016;11(8):e0161169.  2016
  23. Defects in Stratum Corneum Desquamation Are the Predominant Effect of Impaired ABCA12 Function in a Novel Mouse Model of Harlequin Ichthyosis
    PLoS ONE, 2016;11(8):e0161465.  2016
  24. Naturally Occurring Autoantibodies against Tau Protein Are Reduced in Parkinson's Disease Dementia
    PLoS ONE, 2016;11(11):e0164953.  2016
  25. Impact of Histone H1 on the Progression of Allergic Rhinitis and Its Suppression by Neutralizing Antibody in Mice
    Authors: T Nakano, R Kamei, T Fujimura, Y Takaoka, A Hori, CY Lai, KC Chiang, Y Shimada, N Ohmori, T Goto, K Ono, CL Chen, S Goto, S Kawamoto
    PLoS ONE, 2016;11(4):e0153630.  2016
  26. A widespread sequence-specific mRNA decay pathway mediated by hnRNPs A1 and A2/B1
    Genes Dev, 2016;30(9):1070-85.  2016
  27. Effects of neuregulin GGF2 (cimaglermin alfa) dose and treatment frequency on left ventricular function in rats following myocardial infarction
    Authors: Tom J Parry
    Eur. J. Pharmacol, 2016;796(0):76-89.  2016
  28. The effects of two polymorphisms on p21cip1 function and their association with Alzheimer's disease in a population of European descent.
    Authors: Yates, Sharon C, Zafar, Amen, Rabai, Erzsebet, Foxall, James B, Nagy, Sheila, Morrison, Karen E, Clarke, Carl, Esiri, Margaret, Christie, Sharon, Smith, A David, Nagy, Zsuzsann
    PLoS ONE, 2015;10(1):e0114050.  2015
  29. Interleukin-22 is increased in multiple sclerosis patients and targets astrocytes.
    Authors: Perriard G, Mathias A, Enz L, Canales M, Schluep M, Gentner M, Schaeren-Wiemers N, Du Pasquier R
    J Neuroinflammation, 2015;12(0):119.  2015
  30. Early decline in serum phospho-CSE1L levels in vemurafenib/sunitinib-treated melanoma and sorafenib/lapatinib-treated colorectal tumor xenografts.
    Authors: Lee W, Shen S, Shih Y, Chou C, Tseng J, Chin S, Liu K, Chen Y, Jiang M
    J Transl Med, 2015;13(0):191.  2015
  31. One-year timeline kinetics of cytokine-mediated cellular immunity in dogs vaccinated against visceral leishmaniasis.
    Authors: Costa-Pereira C, Moreira M, Soares R, Marteleto B, Ribeiro V, Franca-Dias M, Cardoso L, Viana K, Giunchetti R, Martins-Filho O, Araujo M
    BMC Vet Res, 2015;11(0):92.  2015
  32. Upregulated interleukin-21 receptor on B cells associated with the downregulation of IgE in patients with allergic rhinitis.
    Authors: Jen, Hsiao-Yu, Yang, Yao-Hsu, Chiang, Bor-Luen, Chuang, Ya-Hui
    J Interferon Cytokine Res, 2015;35(1):42-8.  2015
  33. Dendritic cells induce Th2-mediated airway inflammatory responses to house dust mite via DNA-dependent protein kinase.
    Authors: Mishra, Amarjit, Brown, Alexandr, Yao, Xianglan, Yang, Shutong, Park, Sung-Jun, Liu, Chengyu, Dagur, Pradeep, McCoy, J Philip, Keeran, Karen J, Nugent, Gayle Z, Jeffries, Kenneth, Qu, Xuan, Yu, Zu-Xi, Levine, Stewart, Chung, Jay H
    Nat Commun, 2015;6(0):6224.  2015
  34. Crucial role of IL1beta and C3a in the in vitro-response of multipotent mesenchymal stromal cells to inflammatory mediators of polytrauma.
    Authors: Hengartner N, Fiedler J, Schrezenmeier H, Huber-Lang M, Brenner R
    PLoS ONE, 2015;10(1):e0116772.  2015
  35. Citrullination and proteolytic processing of chemokines by Porphyromonas gingivalis.
    Authors: Moelants, Eva A V, Loozen, Gitte, Mortier, Anneleen, Martens, Erik, Opdenakker, Ghislain, Mizgalska, Danuta, Szmigielski, Borys, Potempa, Jan, Van Damme, Jo, Teughels, Wim, Proost, Paul
    Infect Immun, 2014;82(6):2511-9.  2014
  36. Modulation of endotoxicity of Shigella generalized modules for membrane antigens (GMMA) by genetic lipid A modifications: relative activation of TLR4 and TLR2 pathways in different mutants.
    Authors: Rossi O, Pesce I, Giannelli C, Aprea S, Caboni M, Citiulo F, Valentini S, Ferlenghi I, MacLennan C, D'Oro U, Saul A, Gerke C
    J Biol Chem, 2014;289(36):24922-35.  2014
  37. Glucopyranosyl lipid A adjuvant significantly enhances HIV specific T and B cell responses elicited by a DNA-MVA-protein vaccine regimen.
    Authors: McKay P, Cope A, Mann J, Joseph S, Esteban M, Tatoud R, Carter D, Reed S, Weber J, Shattock R
    PLoS ONE, 2014;9(1):e84707.  2014
  38. Genome-wide identification and quantification of protein synthesis in cultured cells and whole tissues by puromycin-associated nascent chain proteomics (PUNCH-P).
    Authors: Aviner, Ranen, Geiger, Tamar, Elroy-Stein, Orna
    Nat Protoc, 2014;9(4):751-60.  2014
  39. Distinct patterns of IgG and IgA against food and microbial antigens in serum and feces of patients with inflammatory bowel diseases.
    Authors: Frehn L, Jansen A, Bennek E, Mandic A, Temizel I, Tischendorf S, Verdier J, Tacke F, Streetz K, Trautwein C, Sellge G
    PLoS ONE, 2014;9(9):e106750.  2014
  40. Proteome-wide screening reveals immunodominance in the CD8 T cell response against classical swine fever virus with antigen-specificity dependent on MHC class I haplotype expression.
    Authors: Franzoni G, Kurkure N, Essler S, Pedrera M, Everett H, Bodman-Smith K, Crooke H, Graham S
    PLoS ONE, 2013;8(12):e84246.  2013
  41. Administration of a Toll-like receptor 9 agonist decreases the proviral reservoir in virologically suppressed HIV-infected patients.
    Authors: Winckelmann A, Munk-Petersen L, Rasmussen T, Melchjorsen J, Hjelholt T, Montefiori D, Ostergaard L, Sogaard O, Tolstrup M
    PLoS ONE, 2013;8(4):e62074.  2013
  42. A "prime-pull" vaccine strategy has a modest effect on local and systemic antibody responses to HIV gp140 in mice.
    Authors: Tregoning, John S, Buffa, Viviana, Oszmiana, Anna, Klein, Katja, Walters, Adam A, Shattock, Robin J
    PLoS ONE, 2013;8(11):e80559.  2013
  43. A Monoclonal Antibody-GDNF Fusion Protein Is Not Neuroprotective and Is Associated with Proliferative Pancreatic Lesions in Parkinsonian Monkeys.
    Authors: Ohshima-Hosoyama S, Simmons HA, Goecks N
    PLoS ONE, 2012;7(6):e39036.  2012
  44. Natural IgM is required for suppression of inflammatory arthritis by apoptotic cells.
    Authors: Notley CA, Brown MA, Wright GP, Ehrenstein MR
    J. Immunol., 2011;186(8):4967-72.  2011
  45. IL-1F5, -F6, -F8, and -F9: A Novel IL-1 Family Signaling System That Is Active in Psoriasis and Promotes Keratinocyte Antimicrobial Peptide Expression.
    Authors: Johnston A, Xing X, Guzman AM, Riblett M, Loyd CM, Ward NL, Wohn C, Prens EP, Wang F, Maier LE, Kang S, Voorhees JJ, Elder JT, Gudjonsson JE
    J. Immunol., 2011;186(4):2613-22.  2011
  46. Combined Flt3L/TK gene therapy induces immunological surveillance which mediates an immune response against a surrogate brain tumor neoantigen.
    Authors: King GD, Muhammad AK, Larocque D
    Mol. Ther., 2011;19(10):1793-801.  2011
  47. Glial growth factor 2 promotes functional recovery with treatment initiated up to 7 days after permanent focal ischemic stroke.
    Authors: Iaci JF, Ganguly A, Finklestein SP, Parry TJ, Ren J, Saha S, Sietsma DK, Srinivas M, Vecchione AM, Caggiano AO
    Neuropharmacology, 2010;59(7):640-9.  2010
  48. Macrophage migration inhibitory factor anti-thrombin III complexes are decreased in bladder cancer patient serum: Complex formation as a mechanism of inactivation.
    Authors: Meyer-Siegler KL, Cox J, Leng L, Bucala R, Vera PL
    Cancer Lett., 2010;290(1):49-57.  2010
  49. Expression of type III interferon (IFN) in the vaginal mucosa is mediated primarily by dendritic cells and displays stronger dependence on NF-kappaB than type I IFNs.
    Authors: Iversen MB, Ank N, Melchjorsen J, Paludan SR
    J. Virol., 2010;84(9):4579-86.  2010
  50. Tumoural expression and circulating level of VEGFR-3 (Flt-4) in metastatic melanoma patients: correlation with clinical parameters and outcome.
    Authors: Mouawad R, Spano JP, Comperat E, Capron F, Khayat D
    Eur. J. Cancer, 2009;45(8):1407-14.  2009
  51. Oxidized alpha1-antitrypsin stimulates the release of monocyte chemotactic protein-1 from lung epithelial cells: potential role in emphysema.
    Authors: Li Z, Alam S, Wang J, Sandstrom CS, Janciauskiene S, Mahadeva R
    Am. J. Physiol. Lung Cell Mol. Physiol., 2009;297(2):L388-400.  2009
  52. Nanobacteria are mineralo fetuin complexes.
    Authors: Raoult D, Drancourt M, Azza S, Nappez C, Guieu R, Rolain JM, Fourquet P, Campagna B, La Scola B, Mege JL, Mansuelle P, Lechevalier E, Berland Y, Gorvel JP, Renesto P
    PLoS Pathog., 2008;4(2):e41.  2008
  53. Up-regulated macrophage migration inhibitory factor protects apoptosis of dermal fibroblasts in patients with systemic sclerosis.
    Authors: Kim JY, Kwok SK, Hur KH, Kim HJ, Kim NS, Yoo SA, Kim WU, Cho CS
    Clin. Exp. Immunol., 2008;152(2):328-35.  2008
  54. Facilitated in vivo synthesis of ribonucleic acid and protein via T7 RNA polymerase.
    Authors: Wang TH, Yu SH, Au LC
    Anal. Biochem., 2008;375(1):97-104.  2008
  55. Mycobacterium tuberculosis antigens specifically modulate CCR2 and MCP-1/CCL2 on lymphoid cells from human pulmonary hilar lymph nodes.
    Authors: Arias MA, Jaramillo G, Lopez YP, Mejia N, Mejia C, Pantoja AE, Shattock RJ, Garcia LF, Griffin GE
    J. Immunol., 2007;179(12):8381-91.  2007
  56. Transgenic blockade of interleukin 6 transsignaling abrogates inflammation.
    Authors: Rabe B, Chalaris A, May U, Waetzig GH, Seegert D, Williams AS, Jones SA, Rose-John S, Scheller J
    Blood, 2007;111(3):1021-8.  2007
  57. Nod1-mediated innate immune recognition of peptidoglycan contributes to the onset of adaptive immunity.
    Authors: Fritz JH, Le Bourhis L, Sellge G, Magalhaes JG, Fsihi H, Kufer TA, Collins C, Viala J, Ferrero RL, Girardin SE, Philpott DJ
    Immunity, 2007;26(4):445-59.  2007
  58. Apoptosis is a natural stimulus of IL6R shedding and contributes to the proinflammatory trans-signaling function of neutrophils.
    Authors: Chalaris A, Rabe B, Paliga K, Lange H, Laskay T, Fielding CA, Jones SA, Rose-John S, Scheller J
    Blood, 2007;110(6):1748-55.  2007
  59. Soluble triggering receptor expressed on myeloid cell-1 is increased in patients with ventilator-associated pneumonia: a preliminary report.
    Authors: Horonenko G, Hoyt JC, Robbins RA, Singarajah CU, Umar A, Pattengill J, Hayden JM
    Chest, 2007;132(1):58-63.  2007
  60. Expression and regulation of the orphan receptor RDC1 and its putative ligand in human dendritic and B cells.
    Authors: Infantino S, Moepps B, Thelen M
    J. Immunol., 2006;176(4):2197-207.  2006
  61. VSIG-3 as a ligand of VISTA inhibits human T-cell function.
    Authors: Wang J, Wu G, Manick B, Hernandez V, Renelt M, Erickson C, Guan J, Singh R, Rollins S, Solorz A, Bi M, Li J, Grabowski D, Dirkx J, Tracy C, Stuart T, Ellinghuysen C, Desmond D, Foster C, Kalabokis V
    Immunology, 0;156(1):74-85.  0

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Reviews for Streptavidin-HRP

Average Rating: 4.8 (Based on 12 Reviews)

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Streptavidin-HRP
By Anonymous on 01/22/2020

1:200 dilution in 1% milk used for wesern blot of biotinylated viral protein.
15% gel, wet transfer, overnight incubation with Streptavidin-HRP.
Only specific bands were seen and no background at all.
Highly recommended

Streptavidin-HRP DY998
Streptavidin-HRP
By Anonymous on 11/06/2019
Streptavidin-HRP DY998
Streptavidin-HRP
By Anonymous on 03/29/2019

Used SA-HRP 1:200 for 30 min to compared a in-house biotinylated antibody to the unconjugated with direct ELISA

Streptavidin-HRP DY998
Streptavidin-HRP
By Anonymous on 11/26/2018
Streptavidin-HRP
By Anonymous on 11/06/2018
Streptavidin-HRP
By Anonymous on 08/17/2018
Streptavidin-HRP
By Anonymous on 04/16/2018

We used this Ab for the Elisa in a 1:200 dilution, it works very well, showed no background.

Streptavidin-HRP
By Anonymous on 12/18/2017
Streptavidin-HRP (set of 5 vials enough for 75 plates total)
By Anonymous on 12/05/2017
Streptavidin-HRP (set of 5 vials enough for 75 plates total)
By Anonymous on 07/20/2017

Standard ELISA protocol observed for concentration analysis

Streptavidin-HRP (set of 5 vials enough for 75 plates total) DY998
Streptavidin-HRP (set of 5 vials enough for 75 plates total)
By Anonymous on 12/21/2016

Western blot of biotinated molecule.1:1000dilution of the product in BSA

Streptavidin-HRP (set of 5 vials enough for 75 plates total)
By Anonymous on 06/02/2016