Streptavidin-HRP Summary
INTENDED USE & DESCRIPTION
Streptavidin-HRP (Part # 890803) contains 5 vials (1 mL/vial) of streptavidin conjugated to horseradish peroxidase for use with biotinylated detection antibodies in ELISAs and Western Blotting.
STORAGE & STABILITY
Store unopened at 2-8 °C. Stable for up to 6 months after opening when stored at 2-8 °C. Do Not Freeze.
REAGENT PREPARATION
For use in ELISAs, dilute to the working concentration specified on the vial label using 1% BSA/PBS or Reagent Diluent Concentrate 2 (Catalog # DY995) diluted 1:10 in deionized water. For use in Western blots, the suggested dilution is 1:200-1:2000 in an appropriate diluent. Use the diluted reagent within 60 minutes.
Specifications
Product Datasheets
Citations for Streptavidin-HRP
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Clinical and Analytical Validation of a Novel Urine-Based Test for the Detection of Allograft Rejection in Renal Transplant Patients
Authors: N Nolan, K Valdivieso, R Mani, JYC Yang, RD Sarwal, P Katzenbach, K Chalasani, D Hongo, G Lugtu, C Mark, E Chen, R Nijor, D Savoca, DS Wexler, T Whitson, SJ Huang, LH Lu, RJX Zawada, E Hytopoulos, MM Sarwal
J Clin Med, 2020;9(8):. 2020
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Key residues of the receptor binding motif in the spike protein of SARS-CoV-2 that interact with ACE2 and neutralizing antibodies
Authors: C Yi, X Sun, J Ye, L Ding, M Liu, Z Yang, X Lu, Y Zhang, L Ma, W Gu, A Qu, J Xu, Z Shi, Z Ling, B Sun
Cell. Mol. Immunol., 2020;0(0):. 2020
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New Paradigms to Assess Consequences of Long-Term, Low-Dose Curcumin Exposure in Lung Cancer Cells
Authors: G Smagurausk, J Mahale, K Brown, AL Thomas, LM Howells
Molecules, 2020;25(2):. 2020
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3D Bioprinting of osteochondral tissue substitutes - in vitro-chondrogenesis in multi-layered mineralized constructs
Authors: D Kilian, T Ahlfeld, AR Akkineni, A Bernhardt, M Gelinsky, A Lode
Sci Rep, 2020;10(1):8277. 2020
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Development of a novel &beta-1,6-glucan-specific detection system using functionally-modified recombinant endo-&beta-1,6-glucanase
Authors: D Yamanaka, K Takatsu, M Kimura, M Swamydas, H Ohnishi, T Umeyama, F Oyama, MS Lionakis, N Ohno
J. Biol. Chem., 2020;295(16):5362-5376. 2020
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Dengue virus nonstructural protein 1 activates platelets via Toll-like receptor 4, leading to thrombocytopenia and hemorrhage
Authors: CH Chao, WC Wu, YC Lai, PJ Tsai, GC Perng, YS Lin, TM Yeh
PLoS Pathog., 2019;15(4):e1007625. 2019
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Targeting VLA4 integrin and CXCR2 mobilizes serially repopulating hematopoietic stem cells
Authors: D Karpova, MP Rettig, J Ritchey, D Cancilla, S Christ, L Gehrs, E Chendamara, MO Evbuomwan, M Holt, J Zhang, G Abou-Ezzi, H Celik, E Wiercinska, W Yang, F Gao, LG Eissenberg, RF Heier, SD Arnett, MJ Meyers, MJ Prinsen, DW Griggs, A Trumpp, PG Ruminski, DM Morrow, HB Bonig, DC Link, JF DiPersio
J. Clin. Invest., 2019;129(7):2745-2759. 2019
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Inhibition of FLT1 ameliorates muscular dystrophy phenotype by increased vasculature in a mouse model of Duchenne muscular dystrophy
Authors: M Verma, Y Shimizu-Mo, Y Asakura, JP Ennen, J Bosco, Z Zhou, GH Fong, S Josiah, D Keefe, A Asakura
PLoS Genet., 2019;15(12):e1008468. 2019
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Engineering of CHO cells for the production of vertebrate recombinant sialyltransferases
Authors: B Houeix, MT Cairns
PeerJ, 2019;7(0):e5788. 2019
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Cecropia obtusa extract and chlorogenic acid exhibit anti aging effect in human fibroblasts and keratinocytes cells exposed to UV radiation
Authors: GAD Alves, R Oliveira d, HL Ghislain R, H Masaki, MJV Fonseca
PLoS ONE, 2019;14(5):e0216501. 2019
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Correction of bleeding in experimental severe hemophilia A by systemic delivery of factor VIII-encoding mRNA
Authors: J Russick, S Delignat, P Milanov, O Christophe, G Boros, CV Denis, PJ Lenting, SV Kaveri, S Lacroix-De
Haematologica, 2019;0(0):. 2019
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Cell-Free DNA and CXCL10 Derived from Bronchoalveolar Lavage Predict Lung Transplant Survival
Authors: JYC Yang, SE Verleden, A Zarinsefat, BM Vanaudenae, R Vos, GM Verleden, RD Sarwal, TK Sigdel, JM Liberto, I Damm, D Watson, MM Sarwal
J Clin Med, 2019;8(2):. 2019
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A ribose-functionalized NAD+ with unexpected high activity and selectivity for protein poly-ADP-ribosylation
Authors: XN Zhang, Q Cheng, J Chen, AT Lam, Y Lu, Z Dai, H Pei, NM Evdokimov, SG Louie, Y Zhang
Nat Commun, 2019;10(1):4196. 2019
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Estrogen induces St6gal1 expression and increases IgG sialylation in mice and patients with rheumatoid arthritis: a potential explanation for the increased risk of rheumatoid arthritis in postmenopausal women
Authors: C Engdahl, A Bondt, U Harre, J Raufer, R Pfeifle, A Camponesch, M Wuhrer, M Seeling, IL Mårtensson, F Nimmerjahn, G Krönke, HU Scherer, H Forsblad-d, G Schett
Arthritis Res. Ther., 2018;20(1):84. 2018
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Impact of immunization against OxLDL on the pulmonary response to cigarette smoke exposure in mice
Authors: M Talbot, M Hamel-Auge, MJ Beaulieu, M Gazzola, A Lechasseur, S Aubin, MÈ Paré, D Marsolais, Y Bossé, MC Morissette
Respir. Res., 2018;19(1):131. 2018
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A CpG-adjuvanted intranasal enterovirus 71 vaccine elicits mucosal and systemic immune responses and protects human SCARB2-transgenic mice against lethal challenge
Authors: YL Lin, YH Chow, LM Huang, SM Hsieh, PY Cheng, KC Hu, BL Chiang
Sci Rep, 2018;8(1):10713. 2018
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A systematic analysis of VRC01 antibody expression in Pichia pastoris through signal peptide optimization
Authors: R Aw, PF McKay, RJ Shattock, KM Polizzi
Protein Expr. Purif., 2018;0(0):. 2018
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Structure of the 4-1BB/4-1BBL complex and distinct binding and functional properties of utomilumab and urelumab
Authors: SM Chin, CR Kimberlin, Z Roe-Zurz, P Zhang, A Xu, S Liao-Chan, D Sen, AR Nager, NS Oakdale, C Brown, F Wang, Y Yang, K Lindquist, YA Yeung, S Salek-Arda, J Chaparro-R
Nat Commun, 2018;9(1):4679. 2018
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Hydrogels with precisely controlled integrin activation dictate vascular patterning and�permeability
Authors: S Li, LR Nih, H Bachman, P Fei, Y Li, E Nam, R Dimatteo, ST Carmichael, TH Barker, T Segura
Nat Mater, 2017;16(9):953-961. 2017
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Inhibitory Effects of Polyacetylene Compounds from Panax ginseng on Neurotrophin Receptor-Mediated Hair Growth
Authors: A Suzuki, D Matsuura, H Kanatani, S Yano, M Tsunakawa, S Matsuyama, H Shigemori
Biol. Pharm. Bull., 2017;40(10):1784-1788. 2017
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A novel recombinant antibody specific to full-length stromal derived factor-1 for potential application in biomarker studies
Authors: DI Bromage, S Taferner, M Pillai, DM Yellon, SM Davidson
PLoS ONE, 2017;12(4):e0174447. 2017
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Vitronectin as a Micromanager of Cell Response in Material-Driven Fibronectin Nanonetworks
Authors: M Cantini, K Gomide, V Moulisova, C González-G, M Salmerón-S
Adv Biosyst, 2017;1(9):1700047. 2017
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Development of a Glycosaminoglycan Derived, Selectin Targeting Anti-Adhesive Coating to Treat Endothelial Cell Dysfunction
Authors: JR Wodicka, AM Chambers, GS Sangha, CJ Goergen, A Panitch
Pharmaceuticals (Basel), 2017;10(2):. 2017
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Inhibition of DNA replication by an anti-PCNA aptamer/PCNA complex
Authors: E Kowalska, F Bartnicki, R Fujisawa, P Bonarek, P Hermanowic, T Tsurimoto, K Muszynska, W Strzalka
Nucleic Acids Res., 2017;0(0):. 2017
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Immunization with Live Human Rhinovirus (HRV) 16 Induces Protection in Cotton Rats against HRV14 Infection
Authors: MC Patel, LM Pletneva, MS Boukhvalov, SN Vogel, AE Kajon, JCG Blanco
Front Microbiol, 2017;8(0):1646. 2017
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Exploring Serum Levels of Brain Derived Neurotrophic Factor and Nerve Growth Factor Across Glaucoma Stages
Authors: F Oddone, G Roberti, A Micera, A Busanello, S Bonini, L Quaranta, L Agnifili, G Manni
PLoS ONE, 2017;12(1):e0168565. 2017
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TLR4 and TLR7/8 Adjuvant Combinations Generate Different Vaccine Antigen-Specific Immune Outcomes in Minipigs when Administered via the ID or IN Routes.
Authors: McKay P, King D, Mann J, Barinaga G, Carter D, Shattock R
PLoS ONE, 2016;11(2):e0148984. 2016
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Impact of Histone H1 on the Progression of Allergic Rhinitis and Its Suppression by Neutralizing Antibody in Mice
Authors: T Nakano, R Kamei, T Fujimura, Y Takaoka, A Hori, CY Lai, KC Chiang, Y Shimada, N Ohmori, T Goto, K Ono, CL Chen, S Goto, S Kawamoto
PLoS ONE, 2016;11(4):e0153630. 2016
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Naturally Occurring Autoantibodies against Tau Protein Are Reduced in Parkinson's Disease Dementia
PLoS ONE, 2016;11(11):e0164953. 2016
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Impact of LbSapSal Vaccine in Canine Immunological and Parasitological Features before and after Leishmania chagasi-Challenge
PLoS ONE, 2016;11(8):e0161169. 2016
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Defects in Stratum Corneum Desquamation Are the Predominant Effect of Impaired ABCA12 Function in a Novel Mouse Model of Harlequin Ichthyosis
PLoS ONE, 2016;11(8):e0161465. 2016
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A widespread sequence-specific mRNA decay pathway mediated by hnRNPs A1 and A2/B1
Genes Dev, 2016;30(9):1070-85. 2016
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Effects of neuregulin GGF2 (cimaglermin alfa) dose and treatment frequency on left ventricular function in rats following myocardial infarction
Authors: Tom J Parry
Eur. J. Pharmacol, 2016;796(0):76-89. 2016
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Upregulated interleukin-21 receptor on B cells associated with the downregulation of IgE in patients with allergic rhinitis.
Authors: Jen, Hsiao-Yu, Yang, Yao-Hsu, Chiang, Bor-Luen, Chuang, Ya-Hui
J Interferon Cytokine Res, 2015;35(1):42-8. 2015
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The effects of two polymorphisms on p21cip1 function and their association with Alzheimer's disease in a population of European descent.
Authors: Yates, Sharon C, Zafar, Amen, Rabai, Erzsebet, Foxall, James B, Nagy, Sheila, Morrison, Karen E, Clarke, Carl, Esiri, Margaret, Christie, Sharon, Smith, A David, Nagy, Zsuzsann
PLoS ONE, 2015;10(1):e0114050. 2015
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Interleukin-22 is increased in multiple sclerosis patients and targets astrocytes.
Authors: Perriard G, Mathias A, Enz L, Canales M, Schluep M, Gentner M, Schaeren-Wiemers N, Du Pasquier R
J Neuroinflammation, 2015;12(0):119. 2015
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Dendritic cells induce Th2-mediated airway inflammatory responses to house dust mite via DNA-dependent protein kinase.
Authors: Mishra, Amarjit, Brown, Alexandr, Yao, Xianglan, Yang, Shutong, Park, Sung-Jun, Liu, Chengyu, Dagur, Pradeep, McCoy, J Philip, Keeran, Karen J, Nugent, Gayle Z, Jeffries, Kenneth, Qu, Xuan, Yu, Zu-Xi, Levine, Stewart, Chung, Jay H
Nat Commun, 2015;6(0):6224. 2015
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Early decline in serum phospho-CSE1L levels in vemurafenib/sunitinib-treated melanoma and sorafenib/lapatinib-treated colorectal tumor xenografts.
Authors: Lee W, Shen S, Shih Y, Chou C, Tseng J, Chin S, Liu K, Chen Y, Jiang M
J Transl Med, 2015;13(0):191. 2015
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One-year timeline kinetics of cytokine-mediated cellular immunity in dogs vaccinated against visceral leishmaniasis.
Authors: Costa-Pereira C, Moreira M, Soares R, Marteleto B, Ribeiro V, Franca-Dias M, Cardoso L, Viana K, Giunchetti R, Martins-Filho O, Araujo M
BMC Vet Res, 2015;11(0):92. 2015
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Crucial role of IL1beta and C3a in the in vitro-response of multipotent mesenchymal stromal cells to inflammatory mediators of polytrauma.
Authors: Hengartner N, Fiedler J, Schrezenmeier H, Huber-Lang M, Brenner R
PLoS ONE, 2015;10(1):e0116772. 2015
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Distinct patterns of IgG and IgA against food and microbial antigens in serum and feces of patients with inflammatory bowel diseases.
Authors: Frehn L, Jansen A, Bennek E, Mandic A, Temizel I, Tischendorf S, Verdier J, Tacke F, Streetz K, Trautwein C, Sellge G
PLoS ONE, 2014;9(9):e106750. 2014
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Genome-wide identification and quantification of protein synthesis in cultured cells and whole tissues by puromycin-associated nascent chain proteomics (PUNCH-P).
Authors: Aviner, Ranen, Geiger, Tamar, Elroy-Stein, Orna
Nat Protoc, 2014;9(4):751-60. 2014
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Citrullination and proteolytic processing of chemokines by Porphyromonas gingivalis.
Authors: Moelants, Eva A V, Loozen, Gitte, Mortier, Anneleen, Martens, Erik, Opdenakker, Ghislain, Mizgalska, Danuta, Szmigielski, Borys, Potempa, Jan, Van Damme, Jo, Teughels, Wim, Proost, Paul
Infect Immun, 2014;82(6):2511-9. 2014
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Modulation of endotoxicity of Shigella generalized modules for membrane antigens (GMMA) by genetic lipid A modifications: relative activation of TLR4 and TLR2 pathways in different mutants.
Authors: Rossi O, Pesce I, Giannelli C, Aprea S, Caboni M, Citiulo F, Valentini S, Ferlenghi I, MacLennan C, D'Oro U, Saul A, Gerke C
J Biol Chem, 2014;289(36):24922-35. 2014
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Glucopyranosyl lipid A adjuvant significantly enhances HIV specific T and B cell responses elicited by a DNA-MVA-protein vaccine regimen.
Authors: McKay P, Cope A, Mann J, Joseph S, Esteban M, Tatoud R, Carter D, Reed S, Weber J, Shattock R
PLoS ONE, 2014;9(1):e84707. 2014
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Proteome-wide screening reveals immunodominance in the CD8 T cell response against classical swine fever virus with antigen-specificity dependent on MHC class I haplotype expression.
Authors: Franzoni G, Kurkure N, Essler S, Pedrera M, Everett H, Bodman-Smith K, Crooke H, Graham S
PLoS ONE, 2013;8(12):e84246. 2013
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A "prime-pull" vaccine strategy has a modest effect on local and systemic antibody responses to HIV gp140 in mice.
Authors: Tregoning, John S, Buffa, Viviana, Oszmiana, Anna, Klein, Katja, Walters, Adam A, Shattock, Robin J
PLoS ONE, 2013;8(11):e80559. 2013
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Administration of a Toll-like receptor 9 agonist decreases the proviral reservoir in virologically suppressed HIV-infected patients.
Authors: Winckelmann A, Munk-Petersen L, Rasmussen T, Melchjorsen J, Hjelholt T, Montefiori D, Ostergaard L, Sogaard O, Tolstrup M
PLoS ONE, 2013;8(4):e62074. 2013
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A Monoclonal Antibody-GDNF Fusion Protein Is Not Neuroprotective and Is Associated with Proliferative Pancreatic Lesions in Parkinsonian Monkeys.
Authors: Ohshima-Hosoyama S, Simmons HA, Goecks N
PLoS ONE, 2012;7(6):e39036. 2012
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Natural IgM is required for suppression of inflammatory arthritis by apoptotic cells.
Authors: Notley CA, Brown MA, Wright GP, Ehrenstein MR
J. Immunol., 2011;186(8):4967-72. 2011
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Combined Flt3L/TK gene therapy induces immunological surveillance which mediates an immune response against a surrogate brain tumor neoantigen.
Authors: King GD, Muhammad AK, Larocque D
Mol. Ther., 2011;19(10):1793-801. 2011
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IL-1F5, -F6, -F8, and -F9: A Novel IL-1 Family Signaling System That Is Active in Psoriasis and Promotes Keratinocyte Antimicrobial Peptide Expression.
Authors: Johnston A, Xing X, Guzman AM, Riblett M, Loyd CM, Ward NL, Wohn C, Prens EP, Wang F, Maier LE, Kang S, Voorhees JJ, Elder JT, Gudjonsson JE
J. Immunol., 2011;186(4):2613-22. 2011
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Macrophage migration inhibitory factor anti-thrombin III complexes are decreased in bladder cancer patient serum: Complex formation as a mechanism of inactivation.
Authors: Meyer-Siegler KL, Cox J, Leng L, Bucala R, Vera PL
Cancer Lett., 2010;290(1):49-57. 2010
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Glial growth factor 2 promotes functional recovery with treatment initiated up to 7 days after permanent focal ischemic stroke.
Authors: Iaci JF, Ganguly A, Finklestein SP, Parry TJ, Ren J, Saha S, Sietsma DK, Srinivas M, Vecchione AM, Caggiano AO
Neuropharmacology, 2010;59(7):640-9. 2010
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Expression of type III interferon (IFN) in the vaginal mucosa is mediated primarily by dendritic cells and displays stronger dependence on NF-kappaB than type I IFNs.
Authors: Iversen MB, Ank N, Melchjorsen J, Paludan SR
J. Virol., 2010;84(9):4579-86. 2010
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Tumoural expression and circulating level of VEGFR-3 (Flt-4) in metastatic melanoma patients: correlation with clinical parameters and outcome.
Authors: Mouawad R, Spano JP, Comperat E, Capron F, Khayat D
Eur. J. Cancer, 2009;45(8):1407-14. 2009
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Oxidized alpha1-antitrypsin stimulates the release of monocyte chemotactic protein-1 from lung epithelial cells: potential role in emphysema.
Authors: Li Z, Alam S, Wang J, Sandstrom CS, Janciauskiene S, Mahadeva R
Am. J. Physiol. Lung Cell Mol. Physiol., 2009;297(2):L388-400. 2009
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Facilitated in vivo synthesis of ribonucleic acid and protein via T7 RNA polymerase.
Authors: Wang TH, Yu SH, Au LC
Anal. Biochem., 2008;375(1):97-104. 2008
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Up-regulated macrophage migration inhibitory factor protects apoptosis of dermal fibroblasts in patients with systemic sclerosis.
Authors: Kim JY, Kwok SK, Hur KH, Kim HJ, Kim NS, Yoo SA, Kim WU, Cho CS
Clin. Exp. Immunol., 2008;152(2):328-35. 2008
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Nanobacteria are mineralo fetuin complexes.
Authors: Raoult D, Drancourt M, Azza S, Nappez C, Guieu R, Rolain JM, Fourquet P, Campagna B, La Scola B, Mege JL, Mansuelle P, Lechevalier E, Berland Y, Gorvel JP, Renesto P
PLoS Pathog., 2008;4(2):e41. 2008
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Mycobacterium tuberculosis antigens specifically modulate CCR2 and MCP-1/CCL2 on lymphoid cells from human pulmonary hilar lymph nodes.
Authors: Arias MA, Jaramillo G, Lopez YP, Mejia N, Mejia C, Pantoja AE, Shattock RJ, Garcia LF, Griffin GE
J. Immunol., 2007;179(12):8381-91. 2007
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Soluble triggering receptor expressed on myeloid cell-1 is increased in patients with ventilator-associated pneumonia: a preliminary report.
Authors: Horonenko G, Hoyt JC, Robbins RA, Singarajah CU, Umar A, Pattengill J, Hayden JM
Chest, 2007;132(1):58-63. 2007
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Transgenic blockade of interleukin 6 transsignaling abrogates inflammation.
Authors: Rabe B, Chalaris A, May U, Waetzig GH, Seegert D, Williams AS, Jones SA, Rose-John S, Scheller J
Blood, 2007;111(3):1021-8. 2007
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Nod1-mediated innate immune recognition of peptidoglycan contributes to the onset of adaptive immunity.
Authors: Fritz JH, Le Bourhis L, Sellge G, Magalhaes JG, Fsihi H, Kufer TA, Collins C, Viala J, Ferrero RL, Girardin SE, Philpott DJ
Immunity, 2007;26(4):445-59. 2007
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Apoptosis is a natural stimulus of IL6R shedding and contributes to the proinflammatory trans-signaling function of neutrophils.
Authors: Chalaris A, Rabe B, Paliga K, Lange H, Laskay T, Fielding CA, Jones SA, Rose-John S, Scheller J
Blood, 2007;110(6):1748-55. 2007
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Expression and regulation of the orphan receptor RDC1 and its putative ligand in human dendritic and B cells.
Authors: Infantino S, Moepps B, Thelen M
J. Immunol., 2006;176(4):2197-207. 2006
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VSIG-3 as a ligand of VISTA inhibits human T-cell function.
Authors: Wang J, Wu G, Manick B, Hernandez V, Renelt M, Erickson C, Guan J, Singh R, Rollins S, Solorz A, Bi M, Li J, Grabowski D, Dirkx J, Tracy C, Stuart T, Ellinghuysen C, Desmond D, Foster C, Kalabokis V
Immunology, 0;156(1):74-85. 0
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ACE2 activation protects against cognitive decline and reduces amyloid pathology in the Tg2576 mouse model of Alzheimer's disease.
Authors: Evans C, Miners J, Piva G, Willis C, Heard D, Kidd E, Good M, Kehoe P
Acta Neuropathol, 0;139(3):485-502. 0
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Key residues of the receptor binding motif in the spike protein of SARS-CoV-2 that interact with ACE2 and neutralizing antibodies.
Authors: Yi C, Sun X, Ye J, Ding L, Liu M, Yang Z, Lu X, Zhang Y, Ma L, Gu W, Qu A, Xu J, Shi Z, Ling Z, Sun B
Cell Mol Immunol, 0;17(6):621-630. 0
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Reviews for Streptavidin-HRP
Average Rating: 4.8 (Based on 14 Reviews)
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Used Strep-HRP to develop an IL-25 ELISA.
Works as expected at the suggested concentration.
Very nice results, standards and control show up nicely.
1:200 dilution in 1% milk used for wesern blot of biotinylated viral protein.
15% gel, wet transfer, overnight incubation with Streptavidin-HRP.
Only specific bands were seen and no background at all.
Highly recommended
Used SA-HRP 1:200 for 30 min to compared a in-house biotinylated antibody to the unconjugated with direct ELISA
We used this Ab for the Elisa in a 1:200 dilution, it works very well, showed no background.
Standard ELISA protocol observed for concentration analysis
Western blot of biotinated molecule.1:1000dilution of the product in BSA