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Streptavidin-HRP

R&D Systems | Catalog # DY998

Set of 5 vials enough for 75 plates total
R&D Systems
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Key Product Details

Applications

ELISA

Product Summary for Streptavidin-HRP

INTENDED USE & DESCRIPTION

Streptavidin-HRP (Part # 890803) contains 5 vials (1 mL/vial) of streptavidin conjugated to horseradish peroxidase for use with biotinylated detection antibodies in ELISAs and Western Blotting.

STORAGE & STABILITY

Store unopened at 2-8 °C. Stable for up to 6 months after opening when stored at 2-8 °C. Do Not Freeze.

REAGENT PREPARATION

For use in ELISAs, dilute to the working concentration specified on the vial label using 1% BSA/PBS or Reagent Diluent Concentrate 2 (Catalog # DY995) diluted 1:10 in deionized water. For use in Western blots, the suggested dilution is 1:200-1:2000 in an appropriate diluent. Use the diluted reagent within 60 minutes. 

Formulation, Preparation, and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Streptavidin

Streptavidin is a tetrameric protein purified from Streptomyces sp. that binds very tightly to the vitamin biotin with a Kd of ~ 10-14 mol/l. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.

Alternate Names

SA V1; SA V2; Streptavidin V1; Streptavidin V2

Additional Streptavidin Products

Product Documents for Streptavidin-HRP

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Streptavidin-HRP

For research use only

Citations for Streptavidin-HRP

Customer Reviews for Streptavidin-HRP (26)

4.9 out of 5
26 Customer Ratings
5 Stars
88%
4 Stars
12%
3 Stars
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1 Stars
0%

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  • Streptavidin-HRP
    Name: Anonymous
    Verified Customer | Posted 02/18/2025
    Streptavidin-HRP DY998
  • Streptavidin-HRP
    Name: Anonymous
    Verified Customer | Posted 11/08/2023
    Used it for mouse serum and plasma samples and worked fine. I have used 1.5 hrs of incubation for both the samples.
    Streptavidin-HRP DY998
  • Streptavidin-HRP
    Name: Anonymous
    Verified Customer | Posted 07/24/2023
    I used it for human serum samples at 1:50000 dilution and singaps were great.
    Streptavidin-HRP DY998
  • Streptavidin-HRP
    Name: Anonymous
    Verified Customer | Posted 06/23/2023
    Extracelullar vesicles derived from conditioned media were subjected to CD63 determination by a home-made ELISA. Elution profile after Ion exchange chropmatography
    Streptavidin-HRP DY998
  • Streptavidin-HRP
    Name: Cassie Berta
    Verified Customer | Posted 05/09/2023
    Great HRP use for an ELISA assay. Gave me a great consistent standard curve for a residual assay.
    Streptavidin-HRP DY998
  • Streptavidin-HRP
    Name: Dilanka Ratnayake
    Verified Customer | Posted 01/17/2023
  • Streptavidin-HRP
    Name: Anonymous
    Verified Customer | Posted 11/02/2022
    GOOD SIGNAL FOR ELISA ASSAY.
    Streptavidin-HRP DY998
  • Streptavidin-HRP
    Name: Rob Knight
    Verified Customer | Posted 12/09/2021
    Direct ELISA of permeabilized or intact small extracellular vesicles
    Streptavidin-HRP DY998
  • Streptavidin-HRP
    Name: Anonymous
    Verified Customer | Posted 11/18/2021
    Worked great for ELISA assay. Provides acceptable signal to noise ratio consistently.
    Streptavidin-HRP DY998
  • Streptavidin-HRP
    Name: Anonymous
    Verified Customer | Posted 09/28/2021
  • Streptavidin-HRP
    Name: Ricardo Malvicini
    Verified Customer | Posted 09/10/2021
    Semi-quantification of CD63 by ELISA, in different fractions of ion exchange chromatography isolated EV.
    Streptavidin-HRP DY998
  • Streptavidin-HRP
    Name: Anonymous
    Verified Customer | Posted 05/13/2021
  • Streptavidin-HRP
    Name: Anonymous
    Verified Customer | Posted 10/23/2020
  • Streptavidin-HRP
    Name: Josh Richards
    Verified Customer | Posted 02/26/2020
    Used Strep-HRP to develop an IL-25 ELISA. Works as expected at the suggested concentration. Very nice results, standards and control show up nicely.
    Streptavidin-HRP DY998
  • Streptavidin-HRP
    Name: Shubhankar Ambike
    Verified Customer | Posted 01/22/2020
    1:200 dilution in 1% milk used for wesern blot of biotinylated viral protein. 15% gel, wet transfer, overnight incubation with Streptavidin-HRP. Only specific bands were seen and no background at all. Highly recommended
    Streptavidin-HRP DY998
  • Streptavidin-HRP
    Name: Anonymous
    Verified Customer | Posted 11/06/2019
    Streptavidin-HRP DY998
  • Streptavidin-HRP
    Name: Anonymous
    Verified Customer | Posted 03/29/2019
    Used SA-HRP 1:200 for 30 min to compared a in-house biotinylated antibody to the unconjugated with direct ELISA
    Streptavidin-HRP DY998
  • Streptavidin-HRP
    Name: Anonymous
    Verified Customer | Posted 11/26/2018
  • Streptavidin-HRP
    Name: Ed Lo
    Verified Customer | Posted 11/06/2018
  • Streptavidin-HRP
    Name: Anonymous
    Verified Customer | Posted 08/17/2018
  • Streptavidin-HRP
    Name: Anonymous
    Verified Customer | Posted 04/16/2018
    We used this Ab for the Elisa in a 1:200 dilution, it works very well, showed no background.
  • Streptavidin-HRP
    Name: Balaji Mahender
    Verified Customer | Posted 12/18/2017
  • Streptavidin-HRP (set of 5 vials enough for 75 plates total)
    Name: Anonymous
    Verified Customer | Posted 12/05/2017
  • Streptavidin-HRP (set of 5 vials enough for 75 plates total)
    Name: Anonymous
    Verified Customer | Posted 07/20/2017
    Standard ELISA protocol observed for concentration analysis
    Streptavidin-HRP DY998
  • Streptavidin-HRP (set of 5 vials enough for 75 plates total)
    Name: Anonymous
    Verified Customer | Posted 12/21/2016
    Western blot of biotinated molecule.1:1000dilution of the product in BSA
  • Streptavidin-HRP (set of 5 vials enough for 75 plates total)
    Name: Anonymous
    Verified Customer | Posted 06/02/2016

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