Streptavidin-Phycoerythrin

R&D Systems | Catalog # F0040

R&D Systems
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Key Product Details

Applications

Flow Cytometry

Conjugate

Phycoerythrin

Product Specifications

Applications

Flow Cytometry (10 µL/106 cells)

Spectra Viewer

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Formulation, Preparation, and Storage

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Streptavidin

Streptavidin, a protein of 55,000 Daltons, is derived from Streptomyces avidinii and can bind 4 moles of biotin per mole of protein. The dissociation constant for biotin is approximately 10-15 M. The streptavidin-biotin complex is stable over a wide range of pH and temperatures. Streptavidin lacks carbohydrate residues present in the avidin molecule. This tends to reduce non-specific interactions with surface molecules and, therefore, streptavidin is preferred over avidin in many immunologic assays. Streptavidin can be covalently conjugated to fluorescent dyes and then used as a developer where the primary reagent was biotinylated.

Alternate Names

SA V1; SA V2; Streptavidin V1; Streptavidin V2

Additional Streptavidin Products

Product Documents for Streptavidin-Phycoerythrin

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Streptavidin-Phycoerythrin

For research use only

Citations for Streptavidin-Phycoerythrin

Customer Reviews for Streptavidin-Phycoerythrin (2)

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  • Streptavidin-Phycoerythrin
    Name: Anonymous
    Verified Customer | Posted 08/12/2024
    Streptavidin-phycoerythrin (SA-PE) was used to detect binding of a biotinylated TLR11 antibody on monocyte differentiated dendritic cells. Cells were treated with 100 nM of TLR11 (blue) or isotype (red) biotinylated antibodies for 30 minutes followed by 15 minutes of 5 ul of SA-PE.
    Streptavidin-Phycoerythrin F0040
  • Streptavidin-Phycoerythrin
    Name: Anonymous
    Verified Customer | Posted 10/09/2020
    Streptavidin-Phycoerythrin F0040

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