Human Plexin C1 Biotinylated Antibody

R&D Systems | Catalog # BAF3887

R&D Systems
Discontinued Product
BAF3887 has been discontinued. View all Plexin C1 products.

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all Plexin C1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Plexin C1
Ile49-Ser161
Accession # O60486

Specificity

Detects human Plexin C1 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant mouse (rm) Plexin A1, rmPlexin A2, and rmPlexin A3 is observed and less than 5% cross-reactivity with recombinant human (rh) Plexin B1 is observed, and less than 1% cross-reactivity with rhPlexin D1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human Plexin C1 Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human Plexin C1 (Catalog # 3887-PC)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Plexin C1

Plexin C1, also known as Virus-encoded semaphorin protein receptor (VESPR) and CD232, is a 210 kDa type I transmembrane glycoprotein in the C subfamily of the Plexin family (1, 2). Human Plexin C1 contains a 34 amino acid (aa) signal sequence, a 910 aa extracellular domain (ECD) with one Sema-domain and two cysteine‑rich Met-related sequences (MRS), a 21 aa transmembrane domain and a 603 aa cytoplasmic domain that includes features common to other plexins, including a phosphothreonine site. The human Plexin C1 ECD shares 85%, 82% and 71% aa identity with murine, bovine and opossum Plexin C1, respectively. Plexin C1 is widely expressed in neuronal and non-neuronal fetal and adult tissues (3). In neuronal development, its role is unclear. Semaphorin-7a (Sema 7A, CD108) binds Plexin C1 in vitro, and the two show a similar expression pattern during rat neuronal development. However, in rat, beta 1 integrins rather than Plexin C1 appear to mediate Sema 7A effects on axon outgrowth (4, 5). Plexin C1 does appear to play a role in the partitioning of paraventricular and supraoptic neurons in the hypothalamus, as indicated by specific defects seen in mice deleted for Plexin C1 (6). In the immune system, effects of Sema 7A on T cell-mediated inflammatory responses also appear to be mediated by beta 1 integrins rather than plexins (7). However, Plexin C1 may function to oppose the effect of beta 1 integrins, as it does on Sema 7A-mediated spreading and dendrite formation in melanocytes (8). Plexin C1 is the receptor for the poxvirus (A39R protein) and herpes virus (AHVsema) semaphorin homologs, and mediates activation of monocytes and inhibition of dendritic cell and neutrophil phagocytosis by A39R (2, 9, 10).

References

  1. Negishi, M. et al. (2005) Cell. Mol. Life Sci. 62:1363. 
  2. Comeau, M.R. et al. (1998) Immunity 8:473. 
  3. Perala, N.M. et al. (2005) Gene Expr. Patterns 5:355. 
  4. Pasterkamp, R.J. et al. (2007) BMC Dev. Biol. 7:98. 
  5. Pasterkamp, R.J. et al. (2003) Nature 424:398. 
  6. Xu, C. and C-M. Fan (2007) Mol. Endocrinol. 21:1234.
  7. Suzuki, K. et al. (2007) Nature 446:680.
  8. Scott, G.A. et al. (2007) J. Invest. Dermatol. 128:151.
  9. Walzer, T. et al. (2005) J. Immunol. 174:51.
  10. Walzer, T. et al. (2005) Eur. J. Immunol. 35:391.

Alternate Names

CD232, PLXNC1, VESPR

Entrez Gene IDs

10154 (Human); 54712 (Mouse); 362873 (Rat)

Gene Symbol

PLXNC1

UniProt

O60486

Additional Plexin C1 Products

Product Documents for Human Plexin C1 Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Plexin C1 Biotinylated Antibody

For research use only

Customer Reviews for Human Plexin C1 Biotinylated Antibody

There are currently no reviews for this product. Be the first to review Human Plexin C1 Biotinylated Antibody and earn rewards!

Have you used Human Plexin C1 Biotinylated Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies