The 20S Immunoproteasome is a modified form of the constitutively active 20S Proteasome core particle and is the catalytic subunit of the multicomplex Immunoproteasome. The structure of the 20S Immunoproteasome is similar to the 20S Proteasome, which is composed of 28 nonidentical subunits arranged into four stacked rings. However, during 20S Immunoproteasome assembly, the three catalytic beta subunits, beta 1, beta 2, and beta 5, in the two interior rings of the 20S Proteasome are replaced by three IFN gamma -inducible catalytic subunits: beta 1i/LMP2, beta 2i/MECl1, and beta 5i/LMP7. The 20S Immunoproteasome is commonly associated with the 19S, PA28 alpha / beta, or the PA28 gamma regulatory complexes. 20S Immunoproteasome expression is enriched in antigen presenting cells of the immune system where the 20S Immunoproteasome selectively degrades intracellular proteins in a manner that optimizes the generation of peptides for MHC class I antigen presentation. Selective inhibition of 20S Immunoproteasome proteolytic activity using small molecule inhibitors is being examined for therapeutic intervention in cancer and inflammatory diseases. This protein complex has been purified from Burkitt's lymphoma cells (Ramos, RA1, CRL-1596) following stimulation with IFN-gamma and IL-2.
Human Ramos Cell 20S Immunoproteasome Protein, CF
R&D Systems | Catalog # E-371
Key Product Details
Source
Applications
Product Specifications
Source
Purity
Predicted Molecular Mass
Activity
The human 20S Immunoproteasome is able to degrade substrates in an ATP-independent manner in vitro. It can be activated chemically with SDS (0.035%) or by the addition of PA28. Reaction conditions will need to be optimized for each specific application. We recommend an initial i20S Proteasome concentration of 0.5-5 nM.
Formulation, Preparation, and Storage
E-371
| Formulation | X mg/ml (X μM) in 50 mM HEPES pH 7.6, 100 mM NaCl, 1 mM DTT |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: 20S Immunoproteasome
References
- Cascio, P. et al. (2001) EMBO J. 20: 2357
- Ferrington, D.A. & D.S. Gregerson (2012) Prog. Mol. Biol. Transl. Sci. 109: 75
- Kim, H.M. et al. (2011) Biochim. Biophys. Acta 1809: 67
- Kloetzel, P.M. (2001) Nat. Rev. Mol. Cell Biol. 2: 179
- Lee, W. & K.B. Kim (2011) Curr. Top. Med. Chem. 11: 2923
- Niewerth, D., et al. (2014) J Hema & Onc. 7:7
- Stadtmueller, B.M. & C.P. Hill (2011) Mol. Cell 41: 8
- Xie, Y. (2010) J. Mol. Cell Biol. 2: 308
Additional 20S Immunoproteasome Products
Product Documents for Human Ramos Cell 20S Immunoproteasome Protein, CF
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Product Specific Notices for Human Ramos Cell 20S Immunoproteasome Protein, CF
For research use only
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