Human RanGAP1 Alexa Fluor™ Plus 488‑conjugated Antibody

R&D Systems | Catalog # AF7834AFP488

R&D Systems
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Alexa Fluor Plus 488 (Excitation = 493 nm, Emission = 518 nm)

Antibody Source

Polyclonal Sheep IgG
View all RanGAP1 Primary Antibodies »

Product Specifications

Specificity

Detects human RanGAP1 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Human RanGAP1 Alexa Fluor™ Plus 488‑conjugated Antibody

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: RanGAP1

RANGAP-1 (RAN [RAs-related Nuclear protein] GTPase-Activating Protein 1; also Fug1) is a 82-88 kDa member of the RNA1 family of molecules. It is widely expressed, and serves as a regulator of the nuclear regulatory protein RAN. RAN, when bound to GTP, is active, and is involved in the onset and termination of mitosis, plus the import and export of protein into the nucleus. In the cytosol, RAN:GTP is complexed to an Importin:cargo complex. RANGAP-1 converts the RAN‑associated GTP into GDP, and this induces dissociation of the complex into RAN:GDP, Importin and cargo. The cargo stays in the cytosol, while both RAN:GDP and Importin now enter the nucleus. Here, RAN:GDP is converted into RAN:GTP by RCC1, where it binds to a newly formed Importin:cargo complex, and is exported out of the nucleus, completing a cycle. While in the cytoplasm, RANGAP-1 is phosphorylated and stabilized through SUMOlyation and an interaction with Ubc9 and RanBP2. Human RANGAP-1 is 587 amino acids (aa) in length. It contains six LRRs (aa 48-343) plus two utilized acetylation sites and five phosphorylated Ser/Thr, and there is one SUMOylation motif between aa 523-526. There are three potential splice forms. One contains a single Ser insertion after Gly330, while a second contains an alternative start site 55 aa upstream of the standard site. A third isoform possesses that same upstream start site coupled to a 25 aa substitution for aa 525-587. Over aa 418-587, human RANGAP-1 shares 82% aa sequence identity with mouse RANGAP-1.

Long Name

Ran GTPase Activating Protein 1

Alternate Names

Fug1

Entrez Gene IDs

5905 (Human); 19387 (Mouse); 362965 (Rat)

Gene Symbol

RANGAP1

UniProt

P46060 (Human)

Additional RanGAP1 Products

Product Documents for Human RanGAP1 Alexa Fluor™ Plus 488‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human RanGAP1 Alexa Fluor™ Plus 488‑conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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