Retinol-binding protein 4 (RBP4) is a Lipocalin superfamily molecule that transports vitamin A (retinol) and retinaldehyde in the serum. Retinaldehyde is the critical chromophore in the rhodopsin photoreceptor, while both it and retinoic acid regulate a multitude of cellular differentiation and proliferation effects through the intracellular receptors RAR and RXR. RBP4 is secreted primarily by hepatocytes and adipocytes into the blood where the RBP4-retinol complex interacts with transthyretin (TTR). Formation of the complex with TTR increases the serum half-life of RBP4 by preventing RBP4 filtration through the kidney. The C-terminally processed forms of RBP4, which do not bind TTR, are normally excreted into the urine but accumulate in the serum during renal failure. RBP4 promotes hyperglycemia, and its upregulation in visceral and liver adipocytes leads to elevated serum levels.
Human RBP4 Magnetic Luminex Performance Assay
R&D Systems | Catalog # LHK3378
Key Product Details
Assay Type
Format
Species
Analytes Detected
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Product Features
Magnetic Luminex High Performance Assays are flexible bead-based multiplex assays. They allow up to 50 user-defined target analytes to be simultaneously profiled using cell culture supernates, serum, or plasma samples. Some panels are also validated with human milk, saliva, and/or urine samples.
- Simultaneously profile Human RBP4/Retinol-Binding Protein 4 with a panel of related analytes in one sample
- Magnetic format allows for easier wash steps
- Undergoes similar validation testing as Quantikine® ELISA Kits
- Precise and Specific
- Requires a small sample volume (<50 µL)
- Run your assay in just 3-5 hours
- Mass-calibrated standards for consistent results with every new lot of material
- Enough reagents for one 96-well plate
- Quick turn-around time: Kits are typically shipped within 2-3 days of ordering*
*Please allow additional time for international orders.
Performance Validation
R&D Systems Luminex High Performance Assays undergo our most extensive validation testing. All Luminex High Performance Assay Panels are validated for use with serum and plasma samples. Some High Performance Assays are additionally validated for cell culture supernatants, milk, saliva, or urine, as indicated on the individual product data sheets. Luminex High Performance Assays are tested for sensitivity (three-quarters the low standard), intra-assay precision, inter-assay precision, and to ensure assay linearity for validated sample types. Recovery values for individual samples in validated sample types are also tested. Validation data for each analyte can be found in the product datasheet.
Base Kit Contents
- Standard Cocktail(s)
- Bead Diluent
- Biotin Antibody Diluent
- Calibrator Diluent
- Wash Buffer
- Streptavidin-PE
- One flat-bottom 96-well Microplate
- Foil Plate Sealers (4)
- Mixing Bottle
- Standard Value Card**
**A standard curve must be generated each time an assay is run, utilizing values from the Certificate of Analysis provided with each Luminex Assay.
Bead Set Contents
- User-mixed antibody-coated Magnetic beads in individual vials
- User-mixed cocktail of biotinylated detection antibody
Precision
|
Intra-assay Precision (precision within an assay) - Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (precision between assays) - Three samples of known concentration were tested in separate assays to assess precision between assays. |
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| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 67 | 65 | 67 |
| Mean (pg/mL) | 309 | 2116 | 13,990 | 288 | 1978 | 12,875 |
| Standard deviation | 18.7 | 35.5 | 555 | 44.5 | 174 | 1102 |
| CV (%) | 6.1 | 1.7 | 4.0 | 15.4 | 8.8 | 8.6 |
Linearity
|
Samples containing and/or spiked with human RBP4 were serially diluted to evaluate assay linearity. |
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| Serum | EDTA plasma | Heparin plasma | Urine | ||||||
|---|---|---|---|---|---|---|---|---|---|
| 1:2 | Average % of Expected | 92 | 93 | 95 | 86 | ||||
| Range (%) | 85-100 | 89-96 | 92-101 | 83-92 | |||||
| 1:4 | Average % of Expected | 97 | 97 | 97 | 99 | ||||
| Range (%) | 96-100 | 95-99 | 95-98 | 98-101 | |||||
| 1:8 | Average % of Expected | 99 | 98 | 94 | 103 | ||||
| Range (%) | 96-103 | 96-103 | 93-94 | 102-104 | |||||
Which Luminex Assay is Right for Me?
Assays for the Luminex platform are offered as High Performance Assays or Discovery Assays.
High Performance Assays are optimized and validated as complete panels with a focused set of analytes. You can choose from Fixed Panels, Premixed options, or Configurable User Mix formats depending on your needs.
Discovery Assays deliver maximum flexibility, enabling the creation of fully customized panels tailored to your research needs. With universal reagents validated for compatibility across all analytes in the menu, they support endless customization possibilities.
Additional Information About Luminex Assays
All R&D Systems Luminex Assays are designed for use with any Luminex instrument including MAGPIX, Luminex 200, FLEXMAP 3D, and the INTELLIFLEX.
Luminex assays use color-coded, magnetic microparticles coated with analyte-specific antibodies to enable multiplex quantitation. During the assay, standards and samples are added to the wells, where target analytes bind to the capture antibodies. A biotinylated detection antibody cocktail is then introduced, followed by a Streptavidin-PE conjugate that generates a fluorescent signal proportional to the amount of analyte present.
Each microparticle is identified by a unique signature and the signal intensity is measured using a Luminex instrument. These assays are designed to deliver reliable and reproducible results with validated antibody pairs and optimized protocols. They are well suited for applications that require sensitivity, accuracy, and confidence in your data while enabling efficient multiplexing to measure multiple targets simultaneously.
Preparation and Storage
Shipping
Stability & Storage
Background: RBP4/Retinol-Binding Protein 4
Alternate Names
Gene Symbol
Additional RBP4/Retinol-Binding Protein 4 Products
Product Documents for Human RBP4 Magnetic Luminex Performance Assay
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human RBP4 Magnetic Luminex Performance Assay
Luminex® is a registered trademark of Luminex Corporation.
For research use only
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