Human RFNG Antibody

R&D Systems | Catalog # AF7198

R&D Systems
Discontinued Product
AF7198 has been discontinued. View all RFNG products.

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all RFNG Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human RFNG
Ala34-Arg331 (His288Gln)
Accession # Q9Y644

Specificity

Detects human RFNG in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human MFNG is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human RFNG Antibody

Detection of Human RFNG antibody by Western Blot.

Detection of Human RFNG by Western Blot.

Western blot shows recombinant human RFNG (10 ng/lane). PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human RFNG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7198) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for RFNG at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human RFNG Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human RFNG

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: RFNG

RFNG (Radical Fringe N-acetylglucosaminyltransferase; also O-fucosylpeptide 3-beta -N-acetylglucosaminyltransferase) is a 36 kDa member of the glucosyltransferase 31 family of enzymes. It is a nonsecreted Golgi membrane protein that likely transfers beta -D-acetylglucosamine to an O-linked fucose residue on Notch. Activity on Notch increases downstream signaling following both Delta-1 and Jagged-1 binding. RFNG is expressed in differentiated fetal tissues such as liver, skin epithelium and migrating neurons. It is also expressed in adult neurons where its activity may actually inhibit Notch signaling. Human RFNG is a 331 amino acid (aa) type II transmembrane protein. It contains a short six aa cytoplasmic region, plus a 302 aa luminal domain (aa 30-331). There are two potential splice variants, both utilizing alternative start sites. One initiates at Met226, while a second initiates at Met127. Over aa 33-331, human RFNG shares 81% aa sequence identity with mouse RFNG.

Long Name

Radical Fringe O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase

Alternate Names

beta-1,3-N-acetylglucosaminyltransferase radical fringe, EC 2.4.1.222, O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase, radical fringe (Drosophila) homolog, radical fringe homolog, radical fringe homolog (Drosophila), RFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase

Entrez Gene IDs

5986 (Human); 19719 (Mouse)

Gene Symbol

RFNG

UniProt

Q9Y644 (Human)

Additional RFNG Products

Product Documents for Human RFNG Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human RFNG Antibody

For research use only

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