|Detection of Human RFNG by Western Blot. Western blot shows recombinant human RFNG (10 ng/lane). PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human RFNG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7198) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for RFNG at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.|
RFNG (Radical Fringe N-acetylglucosaminyltransferase; also O-fucosylpeptide 3-beta -N-acetylglucosaminyltransferase) is a 36 kDa member of the glucosyltransferase 31 family of enzymes. It is a nonsecreted Golgi membrane protein that likely transfers beta -D-acetylglucosamine to an O-linked fucose residue on Notch. Activity on Notch increases downstream signaling following both Delta-1 and Jagged-1 binding. RFNG is expressed in differentiated fetal tissues such as liver, skin epithelium and migrating neurons. It is also expressed in adult neurons where its activity may actually inhibit Notch signaling. Human RFNG is a 331 amino acid (aa) type II transmembrane protein. It contains a short six aa cytoplasmic region, plus a 302 aa luminal domain (aa 30-331). There are two potential splice variants, both utilizing alternative start sites. One initiates at Met226, while a second initiates at Met127. Over aa 33-331, human RFNG shares 81% aa sequence identity with mouse RFNG.
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