Human RFNG Antibody

Name: Human RFNG Antibody
Brand: R&D Systems
SKU: AF7198

Catalog #: AF7198 Datasheet / COA / SDS

Discontinued Product

AF7198 has been discontinued.

View all RFNG products.

Detection of Human RFNG by Western Blot.

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All RFNG Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human RFNG Antibody Summary

Species Reactivity

Human

Specificity

Detects human RFNG in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human MFNG is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human RFNG
Ala34-Arg331 (His288Gln)
Accession # Q9Y644

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human RFNG by Western Blot. Western blot shows recombinant human RFNG (10 ng/lane). PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human RFNG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7198) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for RFNG at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RFNG

RFNG (Radical Fringe N-acetylglucosaminyltransferase; also O-fucosylpeptide 3-beta -N-acetylglucosaminyltransferase) is a 36 kDa member of the glucosyltransferase 31 family of enzymes. It is a nonsecreted Golgi membrane protein that likely transfers beta -D-acetylglucosamine to an O-linked fucose residue on Notch. Activity on Notch increases downstream signaling following both Delta-1 and Jagged-1 binding. RFNG is expressed in differentiated fetal tissues such as liver, skin epithelium and migrating neurons. It is also expressed in adult neurons where its activity may actually inhibit Notch signaling. Human RFNG is a 331 amino acid (aa) type II transmembrane protein. It contains a short six aa cytoplasmic region, plus a 302 aa luminal domain (aa 30-331). There are two potential splice variants, both utilizing alternative start sites. One initiates at Met226, while a second initiates at Met127. Over aa 33-331, human RFNG shares 81% aa sequence identity with mouse RFNG.

Long Name

Radical Fringe O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase

Entrez Gene IDs

5986 (Human); 19719 (Mouse)

Alternate Names

beta-1,3-N-acetylglucosaminyltransferase radical fringe; EC 2.4.1.222; O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase; radical fringe (Drosophila) homolog; radical fringe homolog (Drosophila); radical fringe homolog; RFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase; RFNG