Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Flow Cytometry

Cited:

Western Blot, ELISA Development

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all ROBO4 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human ROBO4
Gln28-Arg467
Accession # Q8WZ75

Specificity

Detects human ROBO4 in Western blots. In Western blots, less than 2% cross-reactivity with recombinant rat ROBO1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human ROBO4 Biotinylated Antibody

Detection of ROBO4 in HUVEC cells by Flow Cytometry

HUVEC cells were stained with Goat Anti-Human ROBO4 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2366, filled histogram) or isotype control antibody (Catalog # BAF108, open histogram) followed by Streptavidin-Allophycocyanin (Catalog # F0050). View our protocol for Staining Membrane-associated Proteins.

Applications for Human ROBO4 Biotinylated Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: HUVEC human umbilical vein endothelial cells

Western Blot

0.1 µg/mL
Sample: Recombinant Human ROBO4

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ROBO4

ROBO4 belongs to the ROBO family, which are molecular guidance receptors that interact with Slit ligand to regulate axon guidance, neuronal migration and leukocyte chemotaxis. The expression of ROBO4 is restricted in the endothelium, notably in areas of angiogenesis.

Long Name

Roundabout Homolog 4

Alternate Names

ECSM4, FLJ20798, Magic roundabout, MGC133352, MGC133353, MRB, roundabout homolog 4, roundabout homolog 4, magic roundabout (Drosophila)

Entrez Gene IDs

54538 (Human); 74144 (Mouse)

Gene Symbol

ROBO4

UniProt

Q8WZ75

Additional ROBO4 Products

Product Documents for Human ROBO4 Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human ROBO4 Biotinylated Antibody

For research use only

Citations for Human ROBO4 Biotinylated Antibody

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FAQs

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