Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Rat

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all Serpin F1/PEDF Primary Antibodies »

Product Specifications

Immunogen

recombinant human Serpin F1/PEDF

Specificity

Detects human Serpin F1/PEDF in Western blots. In this format, approximately 40% cross-reactivity with recombinant mouse Serpin F1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human Serpin F1/PEDF Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human Serpin F1/PEDF

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Serpin F1/PEDF

Serpin F1 (serine proteinase inhibitor-clade F #1; also PEDF, PIG35 and EPC-1) is a secreted, 50 kDa glycoprotein member of the clade F-subfamily, serpin superfamily of protease inhibitors.  It is expressed by diverse cell types such as retinal pigment and breast epithelium, fibroblasts, astrocytes and hepatocytes.  Serpin F1 circulates in blood and binds to type I collagen plus heparan sulfate.  Although it is a serpin, it belongs to a class of serpins that are nonprotease inhibiting, and instead is known to possess potent antiangiogenic and neurotrophic activity.  Human serpin F1 is 418 amino acids (aa) in length.  It contains a 19 aa signal sequence plus a 399 aa mature region that shows an NLS (aa 146-149), a neuroprotective motif (aa 354-359) and an antiangiogenesis segment (aa 387-411).  Phosphorylation occurs at multiple sites that affect bioactivity.  Over aa 44-418, human serpin F1 shares 87% aa identity with mouse serpin F1.        

Long Name

Serpine F1/Pigment Epithelium-derived Factor

Alternate Names

EPC-1, PEDF

Entrez Gene IDs

5176 (Human); 20317 (Mouse); 287526 (Rat)

Gene Symbol

SERPINF1

Additional Serpin F1/PEDF Products

Product Documents for Human Serpin F1/PEDF Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Serpin F1/PEDF Biotinylated Antibody

For research use only

Related Research Areas

Serine Proteases and Regulators

Citations for Human Serpin F1/PEDF Biotinylated Antibody

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