Surfactant Protein D (SP-D) is a collectin family protein consisting of a collagen-like sequence followed by a C-type lectin domain. It is synthesized primarily in the lung by alveolar type II cells and unciliated bronchial epithelial cells. In addition, SP-D is produced by epithelial cells of the gastrointestinal tract and exocrine glands. SP-D binds to patterns of neutral sugars on micro-organisms to mediate direct opsonization and phagocytosis and is an important component of the innate immune system.
Human SP-D QuicKit ELISA
R&D Systems | Catalog # QK1920
Human SP-D QuicKit ELISA
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Key Product Details
Assay Length
80 minutes
Sample Type & Volume Required Per Well
Cell Culture Supernates (25 µL), Serum (17 µL), EDTA Plasma (17 µL), Heparin Plasma (17 µL), Bronchoalveolar Lavage Fluid (25 µL)
Sensitivity
0.163 ng/mL
Assay Range
0.625-40 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
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Product Summary for Human SP-D QuicKit ELISA
The Quantikine QuicKit Human SP-D Immunoassay is a one-step, 80-minute solid phase ELISA designed to measure human SP-D levels in cell culture supernates, serum, plasma, and BALF. It contains recombinant human SP-D and antibodies raised against the recombinant protein. Results obtained for naturally occurring human SP-D showed linear curves that were parallel to the standard curves obtained using the recombinant QuicKit standards. These results indicate that this kit can be used to determine relative mass values for natural SP-D.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Species
Human
Specificity
Natural and recombinant human SP-D.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Two samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Two samples of known concentration were tested in ten separate assays to assess inter-assay precision. Assays were performed by at least four technicians.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 20 | 20 | 10 | 10 |
| Mean (ng/mL) | 4.65 | 23.8 | 4.37 | 22.0 |
| Standard Deviation | 0.210 | 1.27 | 0.277 | 1.62 |
| CV% | 4.5 | 5.3 | 6.3 | 7.4 |
Recovery for Human SP-D QuicKit ELISA
The recovery of human SP-D spiked to three different levels in samples throughout the range of the assay in cell culture media was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Bronchoalveolar Lavage Fluid (n=2) | 96 | 93-99 |
| Cell Culture Media (n=4) | 110 | 106-114 |
| EDTA Plasma (n=2) | 90 | 81-99 |
| Heparin Plasma (n=2) | 86 | 79-95 |
| Serum (n=2) | 90 | 77-100 |
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of human SP-D were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human SP-D QuicKit ELISA
Human SP-D Quantikine QuicKit ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: SP-D
Long Name
Surfactant Pulmonary Associated Protein D
Alternate Names
Collectin 7, PSP-D, SFTP4, SFTPD, SPD
Gene Symbol
SFTPD
Additional SP-D Products
Product Documents for Human SP-D QuicKit ELISA
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human SP-D QuicKit ELISA
For research use only
Related Research Areas
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