Human SUMO1 Antibody

Newer Version Available: A-716

Discontinued Product

A-722 has been discontinued and is replaced by A-716.

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Human SUMO1 Antibody Summary

Specificity
This antibody detects endogenous, human SUMOylated proteins in Western blots. Cross-reactive against murine and rat proteins.
Source
Monoclonal Mouse IgG1 Clone # 21C7
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Purified, recombinant human SUMO1
Accession # P63165
Formulation
Supplied as a solution in PBS containing Glycerol and Sodium Azide.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1-0.5 µg/mL
See below
Immunoprecipitation
Recommended IP conditions: use 10-20 μg of A-722 to recover SUMOylated proteins from 0.5 – 1 mg of total protein in 1 ml of cellular lysate. Use of Sentrin Protease inhibitors such as SUMO1- Vinyl Sulfone may increase yields of recovered SUMOylated substrates.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot View Larger

Western Blot 20 µg of total protein (cellular lysate) obtained from HCT-116 cells, HEK293 cells, or HeLa cells was separated on a reducing 4-20% SDS-PAGE gel. Western blots were developed using PVDF membranes and alpha -SUMO1 (A-722) mAb primary at 0.5 µg/ml followed by HRP-labeled anti-mouse (R&D Systems # HAF007) secondary antibody at 1:2000 dilution. Both SUMO1 protein and SUMOylated RanGAP (confirmed by reprobing membrane with alpha -RanGAP antibody) were detected on the blot.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
  • 6 months from date of receipt, -20 °C as supplied.
  • 3 months, -20 °C under sterile conditions after opening.

Background: SUMO1

Small Ubiquitin­like Modifiers (SUMOs) are a family of small, related proteins that can be enzymatically attached to a target protein by a post­translational modification process termed SUMOylation. Unlike ubiquitination, which targets proteins for degradation, SUMOylation participates in a number of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. All human SUMO proteins share a conserved ubiquitin-like domain and a C-terminal diglycine cleavage/attachment site. Human SUMO1, also known as Sentrin, UBL1, and SMT3C, is synthesized as a 101 amino acid (aa) propeptide that contains a four aa C­terminal prosegment. Following prosegment cleavage, the C­terminal glycine may be enzymatically attached to a lysine on a target protein. Human SUMO1 shares 100% sequence identity to SUMO1 from mouse. SUMO1 is the most unique of the four identified SUMO proteins and shares only 44%, 47%, and 41% sequence identity to SUMO2, SUMO3, and SUMO4, respectively.

Long Name
Small Ubiquitin-like Modifier 1
Entrez Gene IDs
7341 (Human); 22218 (Mouse); 301442 (Rat)
Alternate Names
DAP1; GAP modifying protein 1; GAP-modifying protein 1; GMP1SMT3CSMT3H3OFC10UBL1PIC1; PIC1; SENP2; Sentrin; small ubiquitin-related modifier 1; SMT3 homolog 3; SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae); SMT3 suppressor of mif two 3 homolog 1 (yeast); SMT3; SMT3C; SMT3H3; SUMO1; SUMO-1; Ubiquitin-homology domain protein PIC1; ubiquitin-like 1 (sentrin); Ubiquitin-like protein SMT3C; Ubiquitin-like protein UBL1; UBL1

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