Human SUMO1 Antibody

R&D Systems | Catalog # A-722

R&D Systems
Discontinued Product
A-722 has been discontinued. View all SUMO1 products.

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 21C7
View all SUMO1 Primary Antibodies »

Product Specifications

Immunogen

Purified, recombinant human SUMO1
Accession # P63165

Specificity

This antibody detects endogenous, human SUMOylated proteins in Western blots. Cross-reactive against murine and rat proteins.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human SUMO1 Antibody

20 µg of total protein (cellular lysate) obtained from HCT-116 cells, HEK293 cells, or HeLa cells was separated on a reducing 4-20% SDS-PAGE gel. Western blots were developed using PVDF membranes and alpha -SUMO1 (A-722) mAb primary at 0.5 µg/ml followed by HRP-labeled anti-mouse (R&D Systems # HAF007) secondary antibody at 1:2000 dilution. Both SUMO1 protein and SUMOylated RanGAP (confirmed by reprobing membrane with alpha -RanGAP antibody) were detected on the blot.

Applications for Human SUMO1 Antibody

Application
Recommended Usage

Immunoprecipitation

Recommended IP conditions: use 10-20 μg of A-722 to recover SUMOylated proteins from 0.5 – 1 mg of total protein in 1 ml of cellular lysate. Use of Sentrin Protease inhibitors such as SUMO1- Vinyl Sulfone may increase yields of recovered SUMOylated substrates.

Western Blot

0.1-0.5 µg/mL
Sample: HCT‑116 human colorectal carcinoma cell line, HEK293 human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied as a solution in PBS containing Glycerol and Sodium Azide.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at -20 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Storage below -20 °C is not recommended. Do not use past expiration date.

Background: SUMO1

Small Ubiquitin­like Modifiers (SUMOs) are a family of small, related proteins that can be enzymatically attached to a target protein by a post­translational modification process termed SUMOylation. Unlike ubiquitination, which targets proteins for degradation, SUMOylation participates in a number of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. All human SUMO proteins share a conserved ubiquitin-like domain and a C-terminal diglycine cleavage/attachment site. Human SUMO1, also known as Sentrin, UBL1, and SMT3C, is synthesized as a 101 amino acid (aa) propeptide that contains a four aa C­terminal prosegment. Following prosegment cleavage, the C­terminal glycine may be enzymatically attached to a lysine on a target protein. Human SUMO1 shares 100% sequence identity to SUMO1 from mouse. SUMO1 is the most unique of the four identified SUMO proteins and shares only 44%, 47%, and 41% sequence identity to SUMO2, SUMO3, and SUMO4, respectively.

Long Name

Small Ubiquitin-like Modifier 1

Alternate Names

PIC1, Sentrin, SMT3C, SMT3H3, UBL1

Entrez Gene IDs

7341 (Human); 22218 (Mouse); 301442 (Rat)

Gene Symbol

SUMO1

UniProt

P63165

Additional SUMO1 Products

Product Documents for Human SUMO1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human SUMO1 Antibody

* Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to SDS for additional information and handling instructions.

For research use only

Customer Reviews for Human SUMO1 Antibody

There are currently no reviews for this product. Be the first to review Human SUMO1 Antibody and earn rewards!

Have you used Human SUMO1 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies