This antibody detects endogenous, human SUMOylated proteins in Western blots. Cross-reactive against murine and rat proteins.
Monoclonal Mouse IgG1 Clone # 21C7
Protein A or G purified from hybridoma culture supernatant
Purified, recombinant human SUMO1 Accession # P63165
Supplied as a solution in PBS containing Glycerol and Sodium Azide.
Recommended IP conditions: use 10-20 μg of A-722 to recover SUMOylated proteins from 0.5 – 1 mg of total protein in 1 ml of cellular lysate. Use of Sentrin Protease inhibitors such as SUMO1- Vinyl Sulfone may increase yields of recovered SUMOylated substrates.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Western Blot 20 µg of total protein (cellular lysate) obtained from HCT-116 cells, HEK293 cells, or HeLa cells was separated on a reducing 4-20% SDS-PAGE gel. Western blots were developed using PVDF membranes and alpha -SUMO1 (A-722) mAb primary at 0.5 µg/ml followed by HRP-labeled anti-mouse (R&D Systems # HAF007) secondary antibody at 1:2000 dilution. Both SUMO1 protein and SUMOylated RanGAP (confirmed by reprobing membrane with alpha -RanGAP antibody) were detected on the blot.
Preparation and Storage
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
6 months from date of receipt, -20 °C as supplied.
3 months, -20 °C under sterile conditions after opening.
Small Ubiquitinlike Modifiers (SUMOs) are a family of small, related proteins that can be enzymatically attached to a target protein by a posttranslational modification process termed SUMOylation. Unlike ubiquitination, which targets proteins for degradation, SUMOylation participates in a number of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. All human SUMO proteins share a conserved ubiquitin-like domain and a C-terminal diglycine cleavage/attachment site. Human SUMO1, also known as Sentrin, UBL1, and SMT3C, is synthesized as a 101 amino acid (aa) propeptide that contains a four aa Cterminal prosegment. Following prosegment cleavage, the Cterminal glycine may be enzymatically attached to a lysine on a target protein. Human SUMO1 shares 100% sequence identity to SUMO1 from mouse. SUMO1 is the most unique of the four identified SUMO proteins and shares only 44%, 47%, and 41% sequence identity to SUMO2, SUMO3, and SUMO4, respectively.
Small Ubiquitin-like Modifier 1
Entrez Gene IDs:
7341 (Human); 22218 (Mouse); 301442 (Rat)
DAP1; GAP modifying protein 1; GAP-modifying protein 1; GMP1SMT3CSMT3H3OFC10UBL1PIC1; PIC1; SENP2; Sentrin; small ubiquitin-related modifier 1; SMT3 homolog 3; SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae); SMT3 suppressor of mif two 3 homolog 1 (yeast); SMT3; SMT3C; SMT3H3; SUMO-1; Ubiquitin-homology domain protein PIC1; ubiquitin-like 1 (sentrin); Ubiquitin-like protein SMT3C; Ubiquitin-like protein UBL1; UBL1
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