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Human TACE/ADAM17 Cytosolic Domain Alexa Fluor® 594-conjugated Antibody

    
Datasheet / CoA / SDS
Detection of TACE/ADAM17 in HeLa Human Cell Lineby Flow Cytometry.

Intracellular Staining by Flow Cytometry

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  • Species Reactivity
    Human
  • Specificity
    Detects human TACE/ADAM17 Cytosolic Domain in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human ADAM33 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 136133
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human TACE/ADAM17
    Asp695-Cys824
    Accession # P78536
  • Formulation
    Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 594
Product Datasheets
Product Datasheet

COA

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SDS
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    0.25-1 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Intracellular Staining by Flow Cytometry
Intracellular Staining by Flow Cytometry TACE/ADAM17 Antibody (136133) [Alexa Fluor® 594]
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Detection of TACE/ADAM17 in HeLa Human Cell Lineby Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human TACE/ADAM17 Alexa Fluor® 594-conjugatedMonoclonal Antibody (Catalog # FAB21291T, filled histogram) or isotype controlantibody (Catalog # IC002T, open histogram). To facilitate intracellularstaining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004)and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I(Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: TACE/ADAM17
TACE (also known as ADAM17) is a type I membrane zinc protease of the ADAM family that functions as a major sheddase on the cell surface.
  • Long Name:
    TNF-alpha Converting Enzyme
  • Entrez Gene IDs:
    6868 (Human); 11491 (Mouse)
  • Alternate Names:
    ADAM 17; ADAM metallopeptidase domain 17; ADAM metallopeptidase domain 18; ADAM17; ADAM18; CD156b antigen; CD156b; CSVP; disintegrin and metalloproteinase domain-containing protein 17; EC 3.4.24.86; MGC71942; Snake venom-like protease; TACE; TACEcSVP; TNF-alpha convertase; TNF-alpha converting enzyme; TNF-alpha-converting enzyme; tumor necrosis factor, alpha, converting enzyme
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Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Alexa Fluor® 594-conjugated Antibody

 Ctrl IC002T
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

 Flow FC001 10
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Flow Cytometry Mouse Lyse Buffer (10X)

 Flow FC003 5
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Flow Cytometry Human Lyse Buffer (10X)

 Flow FC002 1
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