Human TACE/ADAM17 Cytosolic Domain Alexa Fluor® 594-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB21291T-100UG
Detection of TACE/ADAM17 in HeLa Human Cell Lineby Flow Cytometry.
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Human TACE/ADAM17 Cytosolic Domain Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human TACE/ADAM17 Cytosolic Domain in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human ADAM33 is observed.
Source
Monoclonal Mouse IgG1 Clone # 136133
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human TACE/ADAM17
Asp695-Cys824
Accession # P78536
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 594

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection of TACE/ADAM17 in HeLa Human Cell Lineby Flow Cytometry. View Larger

Detection of TACE/ADAM17 in HeLa Human Cell Lineby Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human TACE/ADAM17 Alexa Fluor® 594-conjugatedMonoclonal Antibody (Catalog # FAB21291T, filled histogram) or isotype controlantibody (Catalog # IC002T, open histogram). To facilitate intracellularstaining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004)and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I(Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TACE/ADAM17

TACE (also known as ADAM17) is a type I membrane zinc protease of the ADAM family that functions as a major sheddase on the cell surface.

Long Name
TNF-alpha Converting Enzyme
Entrez Gene IDs
6868 (Human); 11491 (Mouse)
Alternate Names
ADAM 17; ADAM metallopeptidase domain 17; ADAM metallopeptidase domain 18; ADAM17; ADAM18; CD156b antigen; CD156b; CSVP; disintegrin and metalloproteinase domain-containing protein 17; EC 3.4.24.86; MGC71942; Snake venom-like protease; TACE; TACEcSVP; TNF-alpha convertase; TNF-alpha converting enzyme; TNF-alpha-converting enzyme; tumor necrosis factor, alpha, converting enzyme

Product Datasheets

FAQs

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