Human TACE/ADAM17 Cytosolic Domain Alexa Fluor® 594-conjugated Antibody

FAB21291T Datasheet / COA / SDS

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FAB21291T-100UG

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Detection of TACE/ADAM17 in HeLa Human Cell Line by Flow Cytometry.

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Human TACE/ADAM17 Cytosolic Domain Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human TACE/ADAM17 Cytosolic Domain in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human ADAM33 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 136133

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human TACE/ADAM17
Asp695-Cys824
Accession # P78536

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Label

Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm)

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

0.25-1 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry

Detection of TACE/ADAM17 antibody in HeLa Human Cell Line antibody by Flow Cytometry.

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Detection of TACE/ADAM17 in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human TACE/ADAM17 Alexa Fluor® 594-conjugated Monoclonal Antibody (Catalog # FAB21291T, filled histogram) or isotype control antibody (Catalog # IC002T, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: TACE/ADAM17

TACE (also known as ADAM17) is a type I membrane zinc protease of the ADAM family that functions as a major sheddase on the cell surface.

Long Name

TNF-alpha Converting Enzyme

Entrez Gene IDs

6868 (Human); 11491 (Mouse)

Alternate Names

ADAM 17; ADAM metallopeptidase domain 17; ADAM metallopeptidase domain 18; ADAM17; ADAM18; CD156b antigen; CD156b; CSVP; disintegrin and metalloproteinase domain-containing protein 17; EC 3.4.24.86; MGC71942; Snake venom-like protease; TACE; TACEcSVP; TNF-alpha convertase; TNF-alpha converting enzyme; TNF-alpha-converting enzyme; tumor necrosis factor, alpha, converting enzyme