Human Themis Alexa Fluor® 405-conjugated Antibody

Catalog # Availability Size / Price Qty
IC6929V-100UG
Product Details
FAQs
Reviews

Human Themis Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Themis in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Themis is observed.
Source
Monoclonal Mouse IgG2a Clone # 701010
Immunogen
E. coli-derived recombinant human Themis
Ala2-Pro282
Accession # Q8N1K5
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
0.25-1 µg/106 cells
Human peripheral blood lymphocytes fixed with paraformaldehyde and permeabilized with saponin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Themis

THEMIS (thymocyte-expressed molecule involved in selection), also called Gasp (Grb2-associating protein), is a 72 kDa, 641 amino acid (aa) cytosolic phosphoprotein mainly expressed in late double-negative and double-positive thymocytes. It is involved in thymocyte positive and negative selection through regulation of TCR signaling. Within the region used as an immunogen, human THEMIS shares 86% aa sequence identity with mouse and rat THEMIS.

Long Name
Thymocyte-expressed Molecule Involved in Selection
Entrez Gene IDs
387357 (Human); 210757 (Mouse)
Alternate Names
bA325O24.3; bA325O24.4; C6orf190; C6orf207; FLJ40584; Gasp; GRB2-associated protein; MGC163388; protein THEMIS; signaling phosphoprotein specific for T cells; SPOT; Themis; thymocyte selection associated; thymocyte selection pathway associated; thymocyte-expressed molecule involved in selection; TSEPA

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human Themis Alexa Fluor® 405-conjugated Antibody

There are currently no reviews for this product. Be the first to review Human Themis Alexa Fluor® 405-conjugated Antibody and earn rewards!

Have you used Human Themis Alexa Fluor® 405-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review