Human TM4SF1/L6 Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB8164G-100UG
Detection of TM4SF1/L6 in A549 Human Cell Line by Flow Cytometry
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Human TM4SF1/L6 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human TM4SF1/L6 in ELISA. In Flow Cytometry, it stains HEK293 cells transfected with human TM4SF1/L6, and does not stain HEK293 cells transfected with irrelevant human cell surface marker.
Source
Monoclonal Mouse IgG1 Clone # 877621
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human TM4SF1/L6
Met1-Cys202
Accession # P30408
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of TM4SF1/L6 in A549 Human Cell Line by Flow Cytometry View Larger

Detection of TM4SF1/L6 in A549 Human Cell Line by Flow Cytometry A549 human lung carcinoma cell line was stained with Mouse Anti-Human TM4SF1/L6 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB8164G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TM4SF1/L6

TM4SF1 (Transmembrane 4 L6 family Member 1; also L6 and M3s1) is a 23-28 kDa member of the L6 tetraspanin family of molecules. It is expressed by fibroblasts, endothelial cells and a variety of tumor cells. TM4SF1 is embedded in both the plasma membrane and the membrane of late endocytic organelles. Here, it appears to be ubiquitinated, and to regulate endocytosis, an action that impacts effective cell migration. TM4SF1 is reported to interact with alpha 5 and beta 1 integrins, and this may impact cell motility. It also suppresses the expression of CD63 and CD82, two molecules that impede cell mobility. Mouse TM4SF1 is a 202 amino acid (aa) 4‑transmembrane (TM) glycoprotein. It contains a short N-terminal cytoplasmic region (aa 1-9), followed by four TM regions and another C-terminal cytoplasmic region (aa 183-202). There is one potential isoform variant that shows an alternative start site at Met60. Over aa 116-161, mouse TM4SF1 shares 73% and 96% aa sequence identity with human and rat TM4SF1, respectively.

Long Name
Transmembrane 4 L Six Family Member 1
Entrez Gene IDs
4071 (Human); 17112 (Mouse)
Alternate Names
L6; M3s1; M3S1Tumor-associated antigen L6; Membrane component chromosome 3 surface marker 1; membrane component, chromosome 3, surface marker 1; TAAL6; TAAL6H-L6; TM4SF1; transmembrane 4 L six family member 1; transmembrane 4 L6 family member 1; transmembrane 4 superfamily member 1

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