Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Flow Cytometry, Immunocytochemistry, CyTOF-ready

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 877621
View all TM4SF1/L6 Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human TM4SF1/L6
Met1-Cys202
Accession # P30408

Specificity

Detects human TM4SF1/L6 in ELISA. In Flow Cytometry, it stains HEK293 cells transfected with human TM4SF1/L6, and does not stain HEK293 cells transfected with irrelevant human cell surface marker.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human TM4SF1/L6 Antibody

Detection of TM4SF1/L6 antibody in A549 Human Cell Line antibody by Flow Cytometry.

Detection of TM4SF1/L6 in A549 Human Cell Line by Flow Cytometry.

A549 human lung carcinoma cell line was stained with Mouse Anti-Human TM4SF1/L6 Monoclonal Antibody (Catalog # MAB8164, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
Detection of TM4SF1/L6 antibody in HEK293 Human Cell Line Transfected with Human TM4SF1/L6 and eGFP antibody by Flow Cytometry.

Detection of TM4SF1/L6 in HEK293 Human Cell Line Transfected with Human TM4SF1/L6 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with human TM4SF1/L6 and eGFP was stained with either (A) Mouse Anti-Human TM4SF1/L6 Monoclonal Antibody (Catalog # MAB8164) or (B) Mouse IgG1Isotype Control (Catalog # MAB002) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
TM4SF1/L6 antibody in A549 Human Cell Line by Immunocytochemistry (ICC).

TM4SF1/L6 in A549 Human Cell Line.

TM4SF1/L6 was detected in immersion fixed A549 human lung carcinoma cell line using Mouse Anti-Human TM4SF1/L6 Monoclonal Antibody (Catalog # MAB8164) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell membranes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

TM4SF1/L6 in HepG2 Human Cell Line.

TM4SF1/L6 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line (positive staining) and HEK293 human embryonic kidney cell line (negative control) using Mouse Anti-Human TM4SF1/L6 Monoclonal Antibody (Catalog # MAB8164) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cell membrane and cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.
TM4SF1/L6 antibody in Human Breast Cancer Tissue by Immunohistochemistry (IHC-P).

TM4SF1/L6 in Human Breast Cancer Tissue.

TM4SF1/L6 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human TM4SF1/L6 Monoclonal Antibody (Catalog # MAB8164) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human TM4SF1/L6 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: A549 human lung carcinoma cell line and HEK293 human embryonic kidney cell line transfected with human TM4SF1/L6 and eGFP

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed A549 human lung carcinoma cell line and immersion fixed HepG2 human hepatocellular carcinoma cell line

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue

Reviewed Applications

Read 1 review rated 5 using MAB8164 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TM4SF1/L6

TM4SF1 (Transmembrane 4 L6 family Member 1; also L6 and M3s1) is a 23-28 kDa member of the L6 tetraspanin family of molecules. It is expressed by fibroblasts, endothelial cells and a variety of tumor cells. TM4SF1 is embedded in both the plasma membrane and the membrane of late endocytic organelles. Here, it appears to be ubiquitinated, and to regulate endocytosis, an action that impacts effective cell migration. TM4SF1 is reported to interact with alpha 5 and beta 1 integrins, and this may impact cell motility. It also suppresses the expression of CD63 and CD82, two molecules that impede cell mobility. Mouse TM4SF1 is a 202 amino acid (aa) 4‑transmembrane (TM) glycoprotein. It contains a short N-terminal cytoplasmic region (aa 1-9), followed by four TM regions and another C-terminal cytoplasmic region (aa 183-202). There is one potential isoform variant that shows an alternative start site at Met60. Over aa 116-161, mouse TM4SF1 shares 73% and 96% aa sequence identity with human and rat TM4SF1, respectively.

Long Name

Transmembrane 4 L Six Family Member 1

Alternate Names

L6, M3s1, TAAL6

Entrez Gene IDs

4071 (Human); 17112 (Mouse)

Gene Symbol

TM4SF1

UniProt

P30408

Additional TM4SF1/L6 Products

Product Documents for Human TM4SF1/L6 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human TM4SF1/L6 Antibody

For research use only

Related Research Areas

Cancer Biomarkers

Citations for Human TM4SF1/L6 Antibody

Customer Reviews for Human TM4SF1/L6 Antibody (1)

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  • Human TM4SF1/L6 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: A549 human lung carcinoma cell line
    Species: Human
    Verified Customer | Posted 02/11/2022
    Human TM4SF1/L6 Antibody MAB8164

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Protocols

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