Human TM4SF1/L6 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human TM4SF1/L6 in ELISA. In Flow Cytometry, it stains HEK293 cells transfected with human TM4SF1/L6, and does not stain HEK293 cells transfected with irrelevant human cell surface marker.
  • Source
    Monoclonal Mouse IgG1 Clone # 877621
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NS0 mouse myeloma cell line transfected with human TM4SF1/L6
    Met1-Cys202
    Accession # P30408
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    2.5 µg/106 cells
    See below
  • Immunohistochemistry
    5-25 µg/mL
    See below
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of TM4SF1/L6 in A549 Human Cell Line by Flow Cytometry. A549 human lung carcinoma cell line was stained with Mouse Anti-Human TM4SF1/L6 Monoclonal Antibody (Catalog # MAB8164, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
Detection of TM4SF1/L6 in HEK293 Human Cell Line Transfected with Human TM4SF1/L6 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human TM4SF1/L6 and eGFP was stained with either (A) Mouse Anti-Human TM4SF1/L6 Monoclonal Antibody (Catalog # MAB8164) or (B) Mouse IgG1 Isotype Control (Catalog # MAB002) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
Immunocytochemistry
TM4SF1/L6 in A549 Human Cell Line. TM4SF1/L6 was detected in immersion fixed A549 human lung carcinoma cell line using Mouse Anti-Human TM4SF1/L6 Monoclonal Antibody (Catalog # MAB8164) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell membranes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunohistochemistry
TM4SF1/L6 in Human Breast Cancer Tissue. TM4SF1/L6 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human TM4SF1/L6 Monoclonal Antibody (Catalog # MAB8164) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TM4SF1/L6

TM4SF1 (Transmembrane 4 L6 family Member 1; also L6 and M3s1) is a 23-28 kDa member of the L6 tetraspanin family of molecules. It is expressed by fibroblasts, endothelial cells and a variety of tumor cells. TM4SF1 is embedded in both the plasma membrane and the membrane of late endocytic organelles. Here, it appears to be ubiquitinated, and to regulate endocytosis, an action that impacts effective cell migration. TM4SF1 is reported to interact with alpha 5 and beta 1 integrins, and this may impact cell motility. It also suppresses the expression of CD63 and CD82, two molecules that impede cell mobility. Mouse TM4SF1 is a 202 amino acid (aa) 4‑transmembrane (TM) glycoprotein. It contains a short N-terminal cytoplasmic region (aa 1-9), followed by four TM regions and another C-terminal cytoplasmic region (aa 183-202). There is one potential isoform variant that shows an alternative start site at Met60. Over aa 116-161, mouse TM4SF1 shares 73% and 96% aa sequence identity with human and rat TM4SF1, respectively.

  • Long Name:
    Transmembrane 4 L Six Family Member 1
  • Entrez Gene IDs:
    4071 (Human); 17112 (Mouse)
  • Alternate Names:
    L6; M3s1; M3S1Tumor-associated antigen L6; Membrane component chromosome 3 surface marker 1; membrane component, chromosome 3, surface marker 1; TAAL6; TAAL6H-L6; TM4SF1/L6; transmembrane 4 L six family member 1; transmembrane 4 L6 family member 1; transmembrane 4 superfamily member 1
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