TMEM119 (Transmembrane Protein 119, also known as Osteoblast Induction Factor or OBIF), is an approximately 38-kDa type 1 transmembrane protein that is predominantly expressed in osteoblasts and is upregulated during osteoblastic differentiation (1, 2). TMEM119 is also expressed in a cell line of microglia, and TMEM119 immunoreactivity is observed in a specific subset of microglia in brains of neurodegenerative diseases, such as Alzheimers disease (3). Mature human TMEM119 consists of a 71 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 166 aa cytoplasmic domain. Within the ECD, human TMEM119 shares 78% and 75% aa sequence identity with mouse and rat TMEM119, respectively. TMEM-119 is involved in the osteoblast differentiation and bone development by acting as a ligand and has been reported to contribute to the proliferation, migration, and invasion of osteosarcoma cells, as well as functioning as an oncogene in osteosarcoma (3, 4).
Human TMEM119 Alexa Fluor® 488‑conjugated Antibody
R&D Systems | Catalog # FAB103131G
Key Product Details
Species Reactivity
Validated:
Cited:
Applications
Validated:
Cited:
Label
Antibody Source
Product Specifications
Immunogen
Arg26-Met96
Accession # Q4V9L6.1
Specificity
Clonality
Host
Isotype
Applications for Human TMEM119 Alexa Fluor® 488‑conjugated Antibody
Flow Cytometry
Sample: HEK293 Human Cell Line Transfected with Human TMEM119 and eGFP
Reviewed Applications
Read 1 review rated 1 using FAB103131G in the following applications:
Spectra Viewer
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Formulation, Preparation, and Storage
Purification
Formulation
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Stability & Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: TMEM119
References
- Jiang, Z,H. et al. (2017) Expt & Mol Med. 49:e329.
- Mizuhashi, K. et al. (2012) Dev. Growth Differ. 54:474.
- Satoh, J. et al. (2016) Neuropathol. 36:39.
- Kanamoto, T. et al. (2009) BMC Develop. Biol. 9:70.
Long Name
Alternate Names
Gene Symbol
UniProt
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Product Documents for Human TMEM119 Alexa Fluor® 488‑conjugated Antibody
Certificate of Analysis
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Product Specific Notices for Human TMEM119 Alexa Fluor® 488‑conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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Citations for Human TMEM119 Alexa Fluor® 488‑conjugated Antibody
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Customer Images
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Application: ImmunocytochemistrySample Tested: Human Brain TissueSpecies: HumanVerified Customer | Posted 09/12/2023The image was captured with a 20X objective in Leica Thunder microscopeIF staining of Alexa Fluor 488 TMEM119 antibody (clone 2699A) on FFPE human brain tissue. For antigen retrieval was used citrate buffer (pH 6) for 15 min using a pressure cooker. Then, the tissue was incubated with the primary antibody at 10 µg/mL overnight at 4°C. Rabbit IgG Isotype Control AF488 was used as a negative control. Nuclei were counterstained with DAPI.
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