Human TRAIL R4/TNFRSF10D PerCP-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human TRAIL R4/TNFRSF10D in ELISAs and Western blots. In sandwich immunoassays, less than 5% cross-reactivity with recombinant human (rh) TRAIL R1, rhTRAIL R2, rhTRAIL R3, rhTRAIL, rhTNF‑ alpha, and rhTNF-beta is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 104918
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human TRAIL R4/TNFRSF10D
    Accession # Q9UBN6
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    PerCP (Peridinin-chlorophyll Protein Complex)
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of TRAIL R4/TNFRSF10D in Human Whole Blood Granulocytes by Flow Cytometry. Human whole blood granulocytes were stained with Mouse Anti-Human TRAIL R4/TNFRSF10D PerCP‑conjugated Monoclonal Antibody (Catalog # FAB633C, filled histogram) or isotype control antibody (Catalog # IC002C, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: TRAIL R4/TNFRSF10D
TRAIL-R4, also known as TRAIL-R4 alpha, Decoy Receptor 2 (DcR2) and TNFRSF10D, is a 39-48 kDa member of the TNFR Superfamily of proteins. Mature TRAIL-R4/DcR2 is a type I transmembrane protein 331 amino acids (aa) in length. It contains a 156 aa extracellular domain (ECD) (aa 56-211), and a 154 aa cytoplasmic region that contains a truncated death domain. The presence of this truncation is not believed to allow for a positive signal for the initiation of apoptosis. This has led to its description as a "decoy receptor", suggesting that its function is to simply sequester and decrease the amount of TRAIL available for binding to the two apoptosis-inducing TRAIL receptors, DR4 and DR5. At this time, this view would appear to be unlikely, as it now seems that DcR2 actually complexes with DR5/TRAIL-R2, blocking the activation of Caspase-8, a first step in the initiation of apoptosis. In addition, in a ligand-dependent manner, the presence of DcR2 has also been associated with the increased survival of select leukocytes. Notably, an isoform variant for TRAIL-R4 (called TRAIL-R4 beta ) has been reported that shows an absence of aa 87-124, a deletion that would render it incapable of binding to TRAIL. Cells reported to express TRAIL-R4 are varied, and include eosinophils, NKT cells, CD8+ (but not CD4+) T cells, colonic columnar epithelium, neutrophils, endometrial columnar epithelium, testicular spermatids and testosterone-secreting Leydig cells, and multiple tumor cell types. A rodent counterpart to TRAIL-R4 has not been reported.
  • Long Name:
    TRAIL Receptor 4
  • Entrez Gene IDs:
    8793 (Human)
  • Alternate Names:
    CD264 antigen; CD264; DcR2; DCR2decoy with truncated death domain; Decoy receptor 2; TNF receptor-related receptor for TRAIL; TNF-related apoptosis-inducing ligand receptor 4; TNFRSF10D; TRAILR4; TRAIL-R4; TRAILR4TRAIL receptor 4; TRUNDDTRAIL receptor with a truncated death domain; tumor necrosis factor receptor superfamily member 10D; tumor necrosis factor receptor superfamily, member 10d, decoy with truncateddeath domain
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 PerCP-conjugated Antibody

Ctrl IC002C  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Human TRAIL R4/TNFRSF10D PerCP-conjugated Antibody?

Submit a review and receive a $25 amazon gift card if you include an image - $10 Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Certificate of Analysis Lookup

The document you requested is not available online. Please enter the Catalog Number and Lot Number below to have a document emailed to you at the address provided

Certificate of Analysis Lookup

Research Areas of Interest
R&D Systems Guarantee


Customer Information