Human TTK Alexa Fluor® 488-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: TTK

TTK (Dual specificity Thr/Tyr kinase; also PYT and hMps1) is a 97-105 kDa member of the Ser/Thr protein kinase family of enzymes. It is expressed in testis and thymus, and participates in cell cycle regulation. In particular, it directs chromosome alignment, centrosome duplication, and spindle assembly checkpoint formation. Via phosphorylation, TTK is known to regulate p53, CDCA8 and TACC2. Human TTK is 857 amino acids (aa) in length. It contains one protein kinase domain (aa 525‑791). Activation occurs following homodimerization and autophosphorylation on Thr675, Thr676 and Thr686. There are two potential splice variants. One shows an alternative start site at Met17, while another shows a deletion of Gln420. Over aa 281-481, human TTK shares 53% aa identity with mouse TTK.  

Product Datasheets