Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 689913
View all TTK Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human TTK
Ser281-Leu481
Accession # P33981

Specificity

Detects human TTK in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) CaM Kinase II, rhCAM Kinase II alpha, rhCaM Kinase II beta, rhCaM Kinase II delta, rhCaM Kinase II gamma, rhCaM Kinase III, rhCaM Kinase IV, rhCaM Kinase K alpha, or rhCaM Kinase K beta is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human TTK Antibody

Detection of Human TTK antibody by Western Blot.

Detection of Human TTK by Western Blot.

Western blot shows lysates of A431 human epithelial carcinoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 2 µg/mL of Human TTK Monoclonal Antibody (Catalog # MAB6028) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TTK at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human TTK Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: A431 human epithelial carcinoma cell line and HeLa human cervical epithelial carcinoma cell line

Reviewed Applications

Read 3 reviews rated 4.7 using MAB6028 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TTK

TTK (Dual specificity Thr/Tyr kinase; also PYT and hMps1) is a 97-105 kDa member of the Ser/Thr protein kinase family of enzymes. It is expressed in testis and thymus, and participates in cell cycle regulation. In particular, it directs chromosome alignment, centrosome duplication, and spindle assembly checkpoint formation. Via phosphorylation, TTK is known to regulate p53, CDCA8 and TACC2. Human TTK is 857 amino acids (aa) in length. It contains one protein kinase domain (aa 525‑791). Activation occurs following homodimerization and autophosphorylation on Thr675, Thr676 and Thr686. There are two potential splice variants. One shows an alternative start site at Met17, while another shows a deletion of Gln420. Over aa 281-481, human TTK shares 53% aa identity with mouse TTK.  

Long Name

TTK Protein Kinase

Alternate Names

Mps1, MPS1L1, PYT

Entrez Gene IDs

7272 (Human); 22137 (Mouse); 315852 (Rat)

Gene Symbol

TTK

UniProt

P33981 (Human)

Additional TTK Products

Product Documents for Human TTK Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human TTK Antibody

For research use only

Customer Reviews for Human TTK Antibody (3)

4.7 out of 5
3 Customer Ratings
5 Stars
67%
4 Stars
33%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Human TTK Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HeLa cells
    Species: Human
    Verified Customer | Posted 12/10/2021
    Human TTK Antibody MAB6028
  • Human TTK Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HepG2
    Species: Human
    Verified Customer | Posted 04/04/2020
    Human TTK Antibody MAB6028
  • Human TTK Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: A431 human epithelial carcinoma cell line and HeLa cells
    Species: Human
    Verified Customer | Posted 10/28/2018
    Human TTK Antibody MAB6028

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