Human Twist-1 Alexa Fluor® 594-conjugated Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
IC6230RT-100UG
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Human Twist-1 Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Twist-1 in direct ELISAs.
Source
Recombinant Monoclonal Mouse IgG1 Clone # 927403R
Purification
Protein A or G purified from cell culture supernatant
Immunogen
E.coli-derived recombinant human Twist-1
Met1-His202
Accession # Q15672
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 594

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
0.25-1 µg/106 cells
A549 human lung carcinoma cell line fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Twist-1

Twist-1 (Twist-related protein 1; also H-Twist and bHLHa38) is a 25‑27 kDa class B member of the bHLH transcription factor family of proteins. It is widely expressed in embryo, and select adult cells such as white adipocytes. In fat, Twist-1 induces fatty acid oxidation via PGC-1 alpha and CPT-1, and promotes MCP-1 and TNF-alpha secretion by adipocytes. In epithelium, Twist-1 dysregulation represses E-Cadherin and induces N-Cadherin expression, resulting in an epithelial-to-mesenchymal transition that can lead to cancer. Human Twist-1 is 202 amino acids (aa) in length. It contains a bHLH domain (aa 109-164) with an embedded DNA-binding motif. Twist-1 forms homodimers, and heterodimerizes with TCF3, HAND 1 and HAND 2. Two distinct mutations exist that may impact its dimerization pattern. There is one seven aa insertion after Ile135, and a second seven aa insertion after Pro139. A third unrelated variant shows a four Gly insert after Gly92. Full-length human Twist-1 shares 98% aa identity with mouse Twist-1.

Long Name
Twist Related Protein 1 [Drosophila]
Entrez Gene IDs
7291 (Human); 22160 (Mouse); 85489 (Rat)
Alternate Names
acrocephalosyndactyly 3; ACS3; B-HLH DNA binding protein; BHLHA38; bHLHa38BPES3; BPES3; Class A basic helix-loop-helix protein 38; CRS1; H-twist; H-twistblepharophimosis, epicanthus inversus and ptosis 3; SCSBPES2; twist homolog 1 (Drosophila); TWIST homolog of drosophila; TWIST; Twist1; Twist-1; twist-related protein 1

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