Ubiquitin+1 has a carboxyl terminal amino acid sequence that differs from normal Ubiquitin. The different carboxyl terminal sequence appears to result from a frameshift in the Ubiquitin mRNA. The underlying mechanisms creating the mRNA frameshift are not clearly understood. The occurrence of the frameshift that generates Ubiquitin+1 is much more prevalent in patients with Alzheimers Disease or with Down Syndrome than in control individuals who are not afflicted with the disorders. The monoclonal anti-Ubiquitin+1 (Catalog # MAB703) and rabbit polyclonal anti-Ubiquitin+1 (Catalog # AF703) antibodies were raised against the Ubiquitin+1 carboxyl terminal sequence that differs from normal Ubiquitin and are therefore non-reactive with Ubiquitin. Monoclonal anti-Ubiquitin (Catalog # MAB701) detects both Ubiquitin and Ubiquitin+1 indicating that the epitope recognized by this antibody is contained in the portion of the proteins that are identical (1, 2).
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
CADLREDPDRQDHHPGSGAQ
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human Ubiquitin+1 Antibody
Detection of Human Ubiquitin+1 by Western Blot.
Western blot shows samples of Recom-binant Human Ubiquitin (Catalog # 701-UB) (2 ng) and Recom-binant Human Ubiquitin+1 (Catalog # 703-UB) (2, 1, and 0.5 ng). PVDF membrane was probed with 1 µg/mL Rabbit Anti-Human Ubiquitin+1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF703) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Ubiquitin+1 was detected at approximately 13 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
Applications for Human Ubiquitin+1 Antibody
Western Blot
Sample: Recombinant Human Ubiquitin+1 (Catalog # 703-UB)
Formulation, Preparation, and Storage
Purification
Reconstitution
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Ubiquitin+1
References
- van Leeuwen, F.W. et al. (1998) Science 279:242.
- van Leeuwen, F.W. et al. (1998) Trends Neurosci. 21:331.
Long Name
Alternate Names
Gene Symbol
Additional Ubiquitin+1 Products
Product Documents for Human Ubiquitin+1 Antibody
Product Specific Notices for Human Ubiquitin+1 Antibody
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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