Human Ubiquitin Antibody

Catalog # Availability Size / Price Qty
A-100
Product Details
Citations (6)
FAQs
Supplemental Products
Reviews

Human Ubiquitin Antibody Summary

Specificity
The antibody cross-reacts with mouse, rat and human ubiquitin; but not with Nedd8 or Sumo1 proteins. This reagent is non-functional for immunoprecipitation.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
Purified human recombinant ubiquitin
Formulation
Supplied as a solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
Recommended dilution range for Western blot analysis is 1:500 to 1:1500. At adilution of 1:1000, 10 ng of monomeric ubiquitin can be detected.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: Ubiquitin

Rabbit polyclonal antibody with a high specificity and affinity for ubiquitin.  The antibody cross-reacts with free ubiquitin, mono-ubiquitin and poly-ubiquitin chain conjugates.

References
    1. Haas, A.L. and Bright, P.M. (1985) J. Biol. Chem 260:12464.
    2. Guarino, L.A. et al. (1995) Cell  80:301-309.
    3. Takada, K. et al. (1995) Eur. J. Biochem.   233:42.
    Entrez Gene IDs
    7314 (Human); 298693 (Rat)
    Alternate Names
    RPS27A; UBA52; UBB ubiquitin B; UBB; UBC; Ubiquitin

    Product Datasheets

    Citations for Human Ubiquitin Antibody

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    6 Citations: Showing 1 - 6
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    1. UBL domain of Usp14 and other proteins stimulates proteasome activities and protein degradation in cells
      Authors: HT Kim, AL Goldberg
      Proc. Natl. Acad. Sci. U.S.A., 2018;115(50):E11642-E11650.
      Applications: Western Blot
    2. THE PHOSPHODIESTERASE-4 INHIBITOR ROFLUMILAST REVERTS PROTEOLYSIS IN SKELETAL MUSCLE CELLS OF PATIENTS WITH COPD CACHEXIA
      Authors: E Barreiro, E Puig-Vilan, A Salazar-De, S Pascual-Gu, C Casadevall, J Gea
      J. Appl. Physiol., 2018;0(0):.
      Species: Human
      Sample Types: Cell Lysates
      Applications: Western Blot
    3. The Deubiquitinating Enzyme Usp14 Allosterically Inhibits Multiple Proteasomal Activities and Ubiquitin-Independent Proteolysis
      Authors: HT Kim, AL Goldberg
      J. Biol. Chem., 2017;0(0):.
      Species: Human
      Sample Types: Protein
      Applications: Western Blot
    4. RNF11 sequestration of the E3 ligase SMURF2 on membranes antagonizes SMAD7 down-regulation of transforming growth factor ? signaling
      Authors: RJ Malonis, W Fu, MJ Jelcic, M Thompson, BS Canter, M Tsikitis, FJ Esteva, I Sánchez
      J. Biol. Chem., 2017;292(18):7435-7451.
      Applications: Western Blot
    5. Prefoldins Negatively Regulate Cold Acclimation in Arabidopsis Thaliana By Promoting Nuclear Proteasome-Mediated HY5 Degradation
      Authors: C Perea-Resa, MA Rodríguez-, E Iniesto, V Rubio, J Salinas
      Mol Plant, 2017;0(0):.
      Species: Plant
      Sample Types: Protein
      Applications: Western Blot
    6. Leucine supplementation improves skeletal muscle regeneration after cryolesion in rats.
      Authors: Pereira, Marcelo, Baptista, Igor L, Carlassara, Eduardo, Moriscot, Anselmo, Aoki, Marcelo, Miyabara, Elen H
      PLoS ONE, 2014;9(1):e85283.
      Species: Rat
      Sample Types: Tissue Homogenates
      Applications: Western Blot

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