Human Ubiquitin Antibody

R&D Systems | Catalog # A-100

R&D Systems
Discontinued Product
A-100 has been discontinued. View all Ubiquitin products.

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Rat, Plant

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Ubiquitin Primary Antibodies »

Product Specifications

Immunogen

Purified human recombinant ubiquitin

Specificity

The antibody cross-reacts with mouse, rat and human ubiquitin; but not with Nedd8 or Sumo1 proteins. This reagent is non-functional for immunoprecipitation.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for Human Ubiquitin Antibody

Application
Recommended Usage

Western Blot

Recommended dilution range for Western blot analysis is 1:500 to 1:1500. At a dilution of 1:1000, 10 ng of monomeric ubiquitin can be detected.

Reviewed Applications

Read 1 review rated 4 using A-100 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied as a solution in PBS.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: Ubiquitin

Rabbit polyclonal antibody with a high specificity and affinity for ubiquitin.  The antibody cross-reacts with free ubiquitin, mono-ubiquitin and poly-ubiquitin chain conjugates.

References

    1. Haas, A.L. and Bright, P.M. (1985) J. Biol. Chem 260:12464.
    2. Guarino, L.A. et al. (1995) Cell  80:301-309.
    3. Takada, K. et al. (1995) Eur. J. Biochem.   233:42.

    Alternate Names

    UBB

    Entrez Gene IDs

    7314 (Human); 298693 (Rat)

    Gene Symbol

    UBB

    Additional Ubiquitin Products

    Product Documents for Human Ubiquitin Antibody

    Certificate of Analysis

    To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

    Note: Certificate of Analysis not available for kit components.

    Download SDS

    Product Specific Notices for Human Ubiquitin Antibody

    For research use only

    Citations for Human Ubiquitin Antibody

    Customer Reviews for Human Ubiquitin Antibody (1)

    4 out of 5
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    • Name: Anonymous
      Application: Western Blot
      Sample Tested: See PMID 19578373
      Species: Other
      Verified Customer | Posted 01/05/2015

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