Human XL Cytokine Luminex® Performance Base Kit

Discontinued Product

LUXLM000 has been discontinued and is replaced by LUXLM000B.

Human XL Cytokine Luminex Panel Standard Curves
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Product Details
Citations (2)
FAQs
Reviews (3)

Human XL Cytokine Luminex® Performance Base Kit Summary

Assay Type bead-based multiplex assay for the Luminex® platform
Format 1 x 96-well microplate and antibody-coated beads
Analytes Detected Please see analyte list in assay customization tool below.
Performance Validation Luminex Assays are validated for use with cell culture supernatants, plasma and serum. All Luminex assays are tested for sensitivity, intra-assay precision, inter-assay precision and to ensure assay linearity for validated sample types. Antibody pairs are selected and tested to confirm the parallel detection of natural and recombinant standard protein and to ensure the accurate determination of target analytes within biological samples. Assays for each target analyte are screened against all target analytes to confirm low antibody cross-reactivity.

View Luminex High Performance Human XL validation data.

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Precision

Intra-Assay Precision - is generated from the mean of the %CV's from 40 reportable results across two different concentrations of analytes in a single serum assay

Inter-Assay Precision - is generated from the mean of the %CV's across two different concentrations of analytes across 25 different serum assays.

  n=40 (20x2 levels) n=25
Analyte Intra-Assay (%CV) Inter-Assay (%CV)
B7-H1/PD-L1 1.8% 11.3%
CCL11/Eotaxin 3.2% 10.0%
CCL19/MIP-3beta 2.2% 9.7%
CCL2/MCP-1 1.4% 8.9%
CCL20/MIP-3 alpha 2.4% 9.9%
CCL3/MIP-1alpha 1.5% 7.3%
CCL4/MIP-1beta 3.0% 9.6%
CD40 Ligand 3.3% 10.6%
CX3CL1/Fractalkine 2.7% 11.1%
CXCL1/GRO alpha 1.0% 8.0%
CXCL10/IP-10 2.1% 8.8%
CXCL2/GRO beta 1.2% 8.8%
CXCL8/IL-8 1.8% 9.0%
EGF 3.0% 9.6%
FGF-basic 4.2% 12.6%
Flt-3 ligand 2.5% 10.1%
G-CSF 1.8% 10.2%
GM-CSF 3.1% 10.3%
Granzyme B 1.8% 11.0%
IFN-alpha 2.9% 9.9%
IFN-beta 3.0% 11.0%
IFN-gamma 2.8% 10.4%
IL-10 3.3% 9.0%
IL-12 p70 2.3% 10.5%
IL-13 1.6% 9.7%
IL-15 2.4% 9.9%
IL-17A 2.5% 11.2%
IL-17E/IL-25 1.7% 9.2%
IL-1alpha 3.0% 10.4%
IL-1beta 2.3% 10.0%
IL-1ra 2.1% 10.3%
IL-2 2.2% 9.2%
IL-3 2.5% 10.1%
IL-33 2.4% 12.3%
IL-4 3.2% 9.7%
IL-5 2.2% 12.0%
IL-6 2.6% 9.8%
IL-7 2.5% 9.8%
PDGF-AA 2.8% 15.5%
PDGF-AB/BB 2.4% 12.4%
RANTES 4.7% 17.4%
TGF-alpha 3.0% 9.8%
TNF-alpha 2.2% 8.7%
TRAIL 2.0% 10.3%
VEGF 2.6% 10.3%

Accuracy

Linearity - The data represents mean spiked or natural linearity in serum matrix samples. Samples with natural linearity are marked with an astrisk.

Spike Recovery - The data represents mean percent recovery of spiked standards ranging from low, medium, and high concentration in serum matrix samples.

  Linearity Spiked
Recovery
Analyte N Average % N Average %

*Natural linearity

B7-H1/PD-L1 4 106% 4 109%
CCL11/Eotaxin 4 92% 4 107%
CCL19/MIP-3beta 4 123% 4 95%
CCL2/MCP-1 4* 82%   High endogenous
CCL20/MIP-3 alpha 4 107% 4 99%
CCL3/MIP-1alpha 4 77% 4 139%
CCL4/MIP-1beta 4 103% 4 111%
CD40 Ligand 4 113% 4 100%
CX3CL1/Fractalkine 4 124% 4 88%
CXCL1/GRO alpha 4 80%   High endogenous
CXCL10/IP-10 3 110%   High endogenous
CXCL2/GRO beta 4* 114% 3 106%
CXCL8/IL-8 4 118% 4 96%
EGF 4* 110% 4 106%
FGF-basic 4 161% 3 80%
Flt-3 ligand 4 102% 4 109%
G-CSF 4 209% 4 43%
GM-CSF 4 96% 4 110%
Granzyme B 4 99% 4 118%
IFN-alpha 3 98% 4 101%
IFN-beta 4 148% 4 69%
IFN-gamma 4 97% 4 112%
IL-10 4 111% 4 104%
IL-12 p70 4 106% 4 108%
IL-13 4 112% 4 103%
IL-15 3 88% 3 89%
IL-17A 4 110% 4 106%
IL-17E/IL-25 4 122% 4 87%
IL-1alpha 4 143% 4 80%
IL-1beta 4 107% 4 99%
IL-1ra 4* 100% 4 145%
IL-2 4 105% 4 102%
IL-3 4 123% 4 88%
IL-33 4 93% 4 110%
IL-4 3 127% 3 77%
IL-5 3 113% 3 81%
IL-6 3 124% 3 90%
IL-7 4 113% 4 96%
PDGF-AA 4* 90%   High endogenous
PDGF-AB/BB 4* 96%   High endogenous
RANTES 4* 96%   High endogenous
TGF-alpha 4 117% 4 96%
TNF-alpha 4 117% 4 69%
TRAIL 3 123% 4 88%
VEGF 4 103% 4 109%
 
Which Luminex Assay is right for me?

Assays for the Luminex platform are offered as High Performance Assays or Assays. The Luminex High Performance Assays are fully validated panels with a focused selection of analytes. They are available in We Mix, You Mix, and Predetermined formats. The Luminex Assays allows for the maximum number of analytes in a multiplex and is supplied as a premixed kit. View a table comparing the features and benefits of these bead-based multiplex assays.

Scientific Data

Human XL Cytokine Luminex Panel Standard Curves

Human XL Cytokine Performance Luminex Panel Serum Sample Values Serum samples from 20 apparently healthy individuals were run on the Human XL Cytokine Luminex High Performance Panel and sample values plotted. Minimum and maximum values shown by exterior lines, the box represents the 1st and 3rd quartiles with the center line representing the median.

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Product Specific Notices
Luminex® is a registered trademark of Luminex Corporation.

Citations for Human XL Cytokine Luminex® Performance Base Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Apoptotic mesenchymal stromal cells support osteoclastogenesis while inhibiting multinucleated giant cells formation in vitro
    Authors: P Humbert, MÁ Brennan, J De Lima, R Brion, A Adrait, C Charrier, B Brulin, V Trichet, Y Couté, F Blanchard, P Layrolle
    Scientific Reports, 2021;11(1):12144.
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Heightened systemic levels of anti-inflammatory cytokines in pulmonary tuberculosis and alterations following anti-tuberculosis treatment
    Authors: K Moideen, NP Kumar, R Bethunaick, VV Banurekha, D Nair, S Babu
    Cytokine, 2019;127(0):154929.
    Species: Human
    Sample Types: Plasma

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Reviews for Human XL Cytokine Luminex® Performance Base Kit

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Luminex Performance Human XL Cytokine Discovery Base Kit
By Anonymous on 10/19/2019
Sample Tested: in vitro cell culture

Human XL Cytokine Luminex Performance Base Kit
By Mark Abrams on 03/28/2018
Sample Tested: human k2edta plasma

Human XL Cytokine Luminex Performance Base Kit
By Mark Abrams on 03/28/2018
Sample Tested: HUMAN K2EDTA PLAMA