HUS1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-89445

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (91%), Rat (92%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all HUS1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: NHFTRISNMIAKLAKTCTLRISPDKLNFILCDKLANGGVSMWCELEQENFFNEFQMEGVSAENNEIYLELTSENLSRASKTAQNARALKIKLTNKHFPCLTVSVELLSMSSSSRIVTHD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HUS1 Antibody - BSA Free

Western Blot: HUS1 Antibody [NBP1-89445]

Western Blot: HUS1 Antibody [NBP1-89445]

Western Blot: HUS1 Antibody [NBP1-89445] - Image was provided by Dr. A-Lien Lu-Chang at Department of Biochemistry & Molecular Biology, University of Maryland School of Medicine.
HUS1 Antibody - BSA Free Immunohistochemistry-Paraffin: HUS1 Antibody - BSA Free [NBP1-89445]

Immunohistochemistry-Paraffin: HUS1 Antibody - BSA Free [NBP1-89445]

Staining of human cervix, uterine moderate nuclear positivity in squamous epithelial cells.
HUS1 Antibody - BSA Free Immunohistochemistry-Paraffin: HUS1 Antibody - BSA Free [NBP1-89445]

Immunohistochemistry-Paraffin: HUS1 Antibody - BSA Free [NBP1-89445]

Staining of human rectum shows moderate nuclear positivity in glandular cells.
HUS1 Antibody - BSA Free Immunohistochemistry-Paraffin: HUS1 Antibody - BSA Free [NBP1-89445]

Immunohistochemistry-Paraffin: HUS1 Antibody - BSA Free [NBP1-89445]

Staining of human prostate shows moderate nuclear positivity in glandular cells.
HUS1 Antibody - BSA Free Immunohistochemistry-Paraffin: HUS1 Antibody - BSA Free [NBP1-89445]

Immunohistochemistry-Paraffin: HUS1 Antibody - BSA Free [NBP1-89445]

Staining of human testis shows strong nuclear positivity in subset of cells in seminiferous ducts.
HUS1 Antibody - BSA Free Western Blot: HUS1 Antibody - BSA Free [NBP1-89445]

Western Blot: HUS1 Antibody - BSA Free [NBP1-89445]

Analysis in control (vector only transfected HEK293T lysate) and HUS1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).

Applications for HUS1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID:32991318).

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HUS1

The protein encoded by the HUS1 gene is a component of an evolutionarily conserved, genotoxin-activated checkpoint complex that is involved in the cell cycle arrest in response to DNA damage. This protein forms a heterotrimeric complex with checkpoint proteins RAD9 and RAD1. In response to DNA damage, the trimeric complex interacts with another protein complex consisting of checkpoint protein RAD17 and four small subunits of the replication factor C (RFC), which loads the combined complex onto the chromatin. The DNA damage induced chromatin binding has been shown to depend on the activation of the checkpoint kinase ATM, and is thought to be an early checkpoint signaling event.

Alternate Names

checkpoint protein HUS1, hHUS1, HUS1 (S. pombe) checkpoint homolog, HUS1 checkpoint homolog (S. pombe), hus1+-like protein

Gene Symbol

HUS1

Additional HUS1 Products

Product Documents for HUS1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for HUS1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for HUS1 Antibody - BSA Free

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Protocols

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FAQs

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