ICAM-1/CD54 Antibody (1H4) [FITC]

Name: ICAM-1/CD54 Antibody (1H4) [FITC]
Brand: Novus Biologicals
SKU: NB500-583
Price: 529 USD
Availability: InStock

Novus Biologicals | Catalog # NB500-583

Submit a review

Product Documents

View Available Conjugates View Available Formulations

100% Guarantee

Product Specifications
Scientific Data
Applications
Spectra Viewer
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Flow (Cell Surface)

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Monoclonal Mouse IgG_2B Clone # 1H4

View all ICAM-1/CD54 Primary Antibodies »

Product Specifications

Immunogen

Raji cells and spleen cells fused with NS1 cells.

Specificity

This antibody (clone 1H4) reacts with CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein (receptor for rhinovirus) expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells; the expression of CD54 is upregulated by activation.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Theoretical MW

110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ICAM-1/CD54 Antibody (1H4) [FITC]

Flow (Cell Surface): ICAM-1/CD54 Antibody (1H4) [FITC] [NB500-583] - Surface staining of human peripheral blood with anti-CD54 (1H4) FITC.

Flow (Cell Surface): ICAM-1/CD54 Antibody (1H4) [FITC] [NB500-583] - Surface staining of U937 human histiocytic lymphoma cell line with anti-human CD54 (1H4) PE. Total viable cells were used for analysis.

Flow Cytometry: ICAM-1/CD54 Antibody (1H4) [FITC] [NB500-583] - Expression of CD54 in normal human peripheral blood, as detected with anti-CD54 (1H4) biotin.

Flow (Cell Surface): ICAM-1/CD54 Antibody (1H4) [FITC] [NB500-583] - Surface staining of human peripheral blood with anti-CD54 (1H4) PerCP.

Flow (Cell Surface): ICAM-1/CD54 Antibody (1H4) [FITC] [NB500-583] - Surface staining of human peripheral blood with anti-CD54 (1H4) APC.

Flow (Cell Surface): ICAM-1/CD54 Antibody (1H4) [FITC] [NB500-583] - Surface staining of human peripheral blood with anti-CD54 (1H4) Alexa Fluor 700.

View 3 more images

Applications for ICAM-1/CD54 Antibody (1H4) [FITC]

Application

Recommended Usage

Flow (Cell Surface)

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

The reagent is designed for Flow Cytometry analysis of human blood cells using 20 ul reagent / 100 ul of whole blood or 10^6 cells in a suspension.

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

Build Your Panel Now

Formulation, Preparation, and Storage

Purification

Size Exclusion Chromatography

Formulation

Phosphate buffered saline (PBS), pH 7.4

Preservative

15mM Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: ICAM-1/CD54

CD54 (ICAM-1) is a 90 kD member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation.

Long Name

Intercellular Adhesion Molecule 1

Alternate Names

CD54, ICAM1

Gene Symbol

ICAM1

Additional ICAM-1/CD54 Products

Product Documents for ICAM-1/CD54 Antibody (1H4) [FITC]

Download Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for ICAM-1/CD54 Antibody (1H4) [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.