Goat F(ab')2 anti-Human IgG F(ab')2 Secondary Antibody [DyLight 649] (Pre-adsorbed)

Novus Biologicals | Catalog # NBP1-51902

Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

DyLight 649 (Excitation = 654 nm, Emission = 673 nm)

Antibody Source

Polyclonal Goat IgG

Format

Pre-adsorbed
View all IgG F(ab')2 Secondary Antibodies »

Product Specifications

Immunogen

Human IgG-F(ab')2 Fragment

Reactivity Notes

Minimum reactivity to mouse and rat.

Specificity

Antiserum was solid phase adsorbed to ensure specificity. Antiserum was cross adsorbed using mouse and rat immunosorbents to remove cross reactive antibodies. The antibody to human F(ab')2 was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to DyLight 649. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. By immunoelectrophoresis and ELISA this antibody reacts specifically with human F(ab')2. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to mouse and rat IgG was detected. This antibody may cross react with F(ab')2 from other species.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images

Flow Cytometry: Goat F(ab')2 anti-Human IgG F(ab')2 Secondary Antibody [DyLight 649] (Pre-adsorbed) [NBP1-51902] - Human cancer cell line. Image from verified customer review.

Applications

Application
Recommended Usage

Flow Cytometry

1:50 - 1:200

Immunocytochemistry/ Immunofluorescence

1:50 - 1:500

Immunohistochemistry

1:50 - 1:500

Immunohistochemistry-Paraffin

1:50 - 1:500

Reviewed Applications

Read 1 review rated 5 using NBP1-51902 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS containing 0.2% BSA

Format

Pre-adsorbed

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Product Documents

Certificate of Analysis

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Product Specific Notices

DyLight 649 is excited at 646 (in PBS) and emits at 674 (in PBS).

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews (1)

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  • Goat F(ab')2 anti-Human IgG (H+L) Secondary Antibody [DyLight 649] (Pre-adsorbed)
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Human cancer cell line
    Species: Human
    Verified Customer | Posted 09/24/2018
    This secondary antibody works fine in my flow cytometry assays. The negative control which incubated with no primary but only this secondary antibody has a very low back ground signal. It binds strong to human Fab.
    Goat F(ab')2 anti-Human IgG F(ab')2 Secondary Antibody [DyLight 649] (Pre-adsorbed) NBP1-51902

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