Chicken IgG F(ab) Isotype Control [HRP]

Novus Biologicals | Catalog # NBP1-97186

Novus Biologicals

Key Product Details

Species Reactivity

Chicken

Applications

SDS-Page

Label

HRP

Antibody Source

Chicken IgG
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Product Specifications

Specificity

No reaction was observed against anti-Chicken IgG F(c) or anti- Papain.

Host

Chicken

Isotype

IgG

Description

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti- Chicken IgG, anti-Chicken IgG F(ab')2 and anti-Chicken Serum

Scientific Data Images for Chicken IgG F(ab) Isotype Control [HRP]

SDS-PAGE: Chicken IgG F(ab) Isotype Control [HRP] [NBP1-97186]

SDS-PAGE: Chicken IgG F(ab) Isotype Control [HRP] [NBP1-97186]

SDS-Page: Chicken IgG F(ab) Isotype Control [HRP] [NBP1-97186] - Lane M: 5 uL Opal Prestained Marker. Lane 1: Reduced Chicken IgG Whole Molecule. Lane 2: Reduced Chicken IgG F(c) Fragment. Lane 3: Reduced Chicken IgG Fab Fragment Peroxidase Conjugated. Lane 4: Reduced Chicken IgM Whole Molecule. Load: 1 ug per lane.

Applications for Chicken IgG F(ab) Isotype Control [HRP]

Application
Recommended Usage

SDS-Page

1:100 - 1:2000
Application Notes
This product has been tested by SDS-PAGE and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.

NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 1.0 ml deionized water (or equivalent).

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative

0.01% Gentamicin Sulfate

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Product Documents for Chicken IgG F(ab) Isotype Control [HRP]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Chicken IgG F(ab) Isotype Control [HRP]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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