Armenian Hamster IgG Isotype Control

Novus Biologicals | Catalog # NBP1-97046

Novus Biologicals
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Key Product Details

Species Reactivity

Armenian Hamster

Applications

Fluorophore-linked immunosorbent assay, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, SDS-Page

Label

Unconjugated

Antibody Source

Armenian Hamster IgG

Format

Purified
View all IgG Isotype Controls »

Product Specifications

Specificity

Greatly diminished reactivity will occur against anti-Golden Syrian Hamster IgG.

Host

Armenian Hamster

Isotype

IgG

Description

This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Armenian Hamster IgG

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for Armenian Hamster IgG Isotype Control

SDS-PAGE: Armenian Hamster IgG Isotype Control [NBP1-97046]

SDS-PAGE: Armenian Hamster IgG Isotype Control [NBP1-97046]

SDS-Page: Armenian Hamster IgG Isotype Control [NBP1-97046] - SDS PAGE Results of Armenian Hamster IgG isotype control. Lane 1: Armenian Hamster IgG Reduced [5.0ug]. Lane 2: Armenian Hamster IgG Reduced [1.0ug]. Lane 3: Lane 4: Armenian Hamster IgG Non-Reduced [1.0ug]. Lane 5: Armenian Hamster IgG Non-Reduced [5.0ug]. 4-20% Gel, Coomassie Stained.

Applications for Armenian Hamster IgG Isotype Control

Application
Recommended Usage

Flow Cytometry

1:1000-1:5000

Fluorophore-linked immunosorbent assay

1:100 - 1:2000

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500
Application Notes
This product has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments. Specific conditions should be optimized by user.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 1.0 ml deionized water (or equivalent).

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

Purified

Preservative

0.01% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IgG

Armenian Hamster isotype controls are used in flow cytometry, western blot and ELISA and differentiate between immunoglobulin classes and subclasses. Isotype controls allow for the genetic variations or differences in the constant regions of the heavy and light chains. In hamster there are six relevant heavy chain isotypes and two light chain isotypes: heavy chain alpha - IgA, gamma - IgG 1, 2a, 2b, 3 and u - IgM, light chain kappa and lambda.

Long Name

Immunoglobulin G

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Gene Symbol

IGHG1

Additional IgG Products

Product Documents for Armenian Hamster IgG Isotype Control

Certificate of Analysis

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Product Specific Notices for Armenian Hamster IgG Isotype Control

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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Protocols

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