Rabbit IgG Isotype Control [PE]

Novus Biologicals | Catalog # NBP2-24983

Novus Biologicals
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Key Product Details

Species Reactivity

Rabbit

Applications

Flow Cytometry

Label

PE (Excitation = 488 nm, Emission = 575 nm)

Antibody Source

Polyclonal Rabbit IgG
View all IgG Isotype Controls »

Product Specifications

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This Rabbit IgG immunoglobulin is useful as an isotype control for Rabbit IgG antibody.

Scientific Data Images for Rabbit IgG Isotype Control [PE]

Flow Cytometry: Rabbit IgG Isotype Control [PE] [NBP2-24983] - FLOW analysis of Mouse CD4+ T cells that were stimulated with anti-CD3/CD28 beads and insulin (1ug/mL) for 5 days in culture media with additional glucose provided. PE conjugated Glut1 antibody stained cells (NB110-39113PE, Orange) were included as positive control which were compared with Rb IgG PE, NBP2-24983 as Isotype Control (Blue) as well as with fluorescence minus one/FMO control (Red) [Image submitted by Verified Customer - see Review for Protocol Details].
Flow Cytometry: Rabbit IgG Isotype Control [PE] [NBP2-24983]

Flow Cytometry: Rabbit IgG Isotype Control [PE] [NBP2-24983]

Flow Cytometry: Rabbit IgG Isotype Control [PE] [NBP2-24983] - PBMCs fixed and permeabilized using the Novus intracellular staining kit and stained with 1 ug of TLR7-PE conjugate (red) or rabbit IgG-PE conjugate isotype control (NBP2-24983, green).

Applications for Rabbit IgG Isotype Control [PE]

Application
Recommended Usage

Flow Cytometry

1 ul/1 million cells
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NBP2-24983 in the following applications:

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please contact technical services for concentration.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: IgG

IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).

Long Name

Immunoglobulin G

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Gene Symbol

IGHG1

Additional IgG Products

Product Documents for Rabbit IgG Isotype Control [PE]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Rabbit IgG Isotype Control [PE]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Citations for Rabbit IgG Isotype Control [PE]

Customer Reviews for Rabbit IgG Isotype Control [PE] (1)

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Customer Images

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  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Murine CD4+ T cells
    Species: Other
    Verified Customer | Posted 02/26/2015
    Glut1 stained Mouse CD4+ Lymphocytes (NB110-39113PE, Orange), Isotype Control (Rb IgG PE, NBP2-24983, Blue) and FMO control(Red)
    Rabbit IgG Isotype Control [PE] NBP2-24983

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FAQs

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