Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

PE/Cy7 (Excitation = 488 nm, Emission = 778 nm)

Antibody Source

Monoclonal Rat IgG2a Kappa Clone # II/41
View all IgM Primary Antibodies »

Product Specifications

Immunogen

The immunogen for this antibody was mouse IgM.

Reactivity Notes

This antibody is reactive to Mouse.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2a Kappa

Scientific Data Images for IgM Antibody (II/41) [PE/Cy7]

Flow Cytometry: Rat anti-Mouse IgM Secondary Antibody (II/41) [PE/Cy7] [NBP1-42940] - Staining of C57BL/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) FITC and 0.06 micrograms of Rat IgG2a ? Isotype Control PE-Cy7 (left) or 0.06 micrograms of Anti-Mouse IgM PE-Cy7 (right). Cells in the lymphocyte gate were used for analysis.

Applications for IgM Antibody (II/41) [PE/Cy7]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
The II/41 antibody has been tested by flow cytometric analysis of mouse spleen cell suspension. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) with 0.1% gelatin

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: IgM

The II/41 monoclonal antibody reacts with the mu heavy chain of mouse IgM. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugated II/41 can be used as a detection secondary for mouse IgM.

Long Name

Immunoglobulin M

Alternate Names

AGM1, DKFZp686I15196, DKFZp686I15212, FLJ00385, immunoglobulin heavy constant mu, MGC104996, MGC52291, MU, VH

Gene Symbol

IGHM

Additional IgM Products

Product Documents for IgM Antibody (II/41) [PE/Cy7]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for IgM Antibody (II/41) [PE/Cy7]

Cy is a registered trademark of Global Life Sciences Solutions Germany GmbH.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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