Mouse IgM Isotype Control (F8)

Novus Biologicals | Catalog # NBP2-21945

Novus Biologicals
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Key Product Details

Species Reactivity

Mouse

Applications

Block/Neutralize, Functional, In vivo assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Clone # F8
View all IgM Isotype Controls »

Product Specifications

Clonality

Monoclonal

Host

Mouse

Isotype

IgM

Description

This Mouse IgM (F8) immunoglobulin is useful as an isotype control for Mouse IgM (F8) antibody. This antibody is purified from culture supernatant of a hybridoma (Mouse myeloma fused with spleen cells from a Mouse, clone F8) with protein A/G beads.

Applications for Mouse IgM Isotype Control (F8)

Application
Recommended Usage

Block/Neutralize

Optimal dilutions of this antibody should be experimentally determined.

Functional

Optimal dilutions of this antibody should be experimentally determined.

In vivo assay

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Use the isotype control at the same concentration as the experimental antibody. Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein L purified

Reconstitution

Reconstitute with PBS.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS. 250 ug size is provided in liquid form, PBS

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IgM

IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and is largely confined to plasma due to its large size. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. Due to its high valency IgM is more efficient than other isotypes is binding antigens with repeating epitopes (virus particles and red blood cells) and is more efficient than IgG in activiating the complement pathway. The gene for the mu constant region contains four domains separated by short intervening sequences. IgM measurement yields information about the body's immediate resistance and response to infection as well as information related to specific diseases. Decreased levels are associated with immune deficiency states, hereditary deficiencies, and myeloma. Increased levels can be associated with Waldenstrom's macroglobulinemia, chronic infection and hepatocellular disease.

Long Name

Immunoglobulin M

Alternate Names

AGM1, DKFZp686I15196, DKFZp686I15212, FLJ00385, immunoglobulin heavy constant mu, MGC104996, MGC52291, MU, VH

Gene Symbol

IGHM

Additional IgM Products

Product Documents for Mouse IgM Isotype Control (F8)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Mouse IgM Isotype Control (F8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Citations for Mouse IgM Isotype Control (F8)

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FAQs

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