IL-27 Antibody

Novus Biologicals | Catalog # NB100-56389

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all IL-27 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to amino acids 31-46 (RPPGRPQLSLQELRRE) of human IL-27 (p28 subunit) of Genbank accession number NP_663634 was used as immunogen.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit IL-27 Antibody (NB100-56389) is a polyclonal antibody validated for use in WB and ICC/IF. Anti-IL-27 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for IL-27 Antibody

Western Blot: IL-27 Antibody [NB100-56389]

Western Blot: IL-27 Antibody [NB100-56389]

Western Blot: IL-27 Antibody [NB100-56389] - Analysis of IL-27 in A) Jurkat and B) NIH 3T3 cells using this antibody at 1 ug/ml.

Applications for IL-27 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

reported in scientific literature (PMID 21294722)

Western Blot

1-2 ug/ml

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS containing 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IL-27

Recently, a new heterodimeric cytokine termed IL-27 that consists of EBI3 (Epstein-Barr virus-induced gene 3), an IL-12p40-related protein, and p28, a newly discovered IL-12p35-related polypeptide has been discovered. IL-27 is involved in early Th1 initiation. Its action is mediated through the orphan cytokine receptor WSX-1/TCCR and gp130. IL-27 may also play a role in the induction of T-bet expression and regulation of Ig class switching in B cells.

Long Name

Interleukin 27

Alternate Names

IL27

Entrez Gene IDs

246778 (Human); 246779 (Mouse)

Gene Symbol

IL27

UniProt

Q8NEV9 (Human)

Additional IL-27 Products

Product Documents for IL-27 Antibody

Certificate of Analysis

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Product Specific Notices for IL-27 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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Associated Pathways

Th1 Differentiation Pathway Th1 Differentiation Pathway Thumbnail