IL-36 beta/IL-1F8 Antibody (1E4) - Azide and BSA Free

Novus Biologicals | Catalog # H00027177-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1E4

Format

Azide and BSA Free
View all IL-36 beta/IL-1F8 Primary Antibodies »

Product Specifications

Immunogen

IL1F8 (NP_775270.1, 1 a.a. ~ 157 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MNPQREAAPKSYAIRDSRQMVWVLSGNSLIAAPLSRSIKPVTLHLIACRDTEFSDKEKGNMVYLGIKGKDLCLFCAEIQGKPTLQLKEKNIMDLYVEKKAQKPFLFFHNKEGSTSVFQSVSYPGWFIATSTTSGQPIFLTKERGITNNTNFYLDSVE

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for IL-36 beta/IL-1F8 Antibody (1E4) - Azide and BSA Free

Western Blot: IL-36 beta/IL-1F8 Antibody (1E4) [H00027177-M01]

Western Blot: IL-36 beta/IL-1F8 Antibody (1E4) [H00027177-M01]

Western Blot: IL1F8 Antibody (1E4) [H00027177-M01] - Analysis of IL1F8 expression in transfected 293T cell line by IL1F8 monoclonal antibody (M01), clone 1E4.Lane 1: IL1F8 transfected lysate(17.7 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: IL1F8 Antibody (1E4) [H00027177-M01] - Detection limit for recombinant GST tagged IL1F8 is 0.1 ng/ml as a capture antibody.

Applications for IL-36 beta/IL-1F8 Antibody (1E4) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is useful for ELISA, Western Blot

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: IL-36 beta/IL-1F8

Interleukin 36 beta (IL-36 beta), also known as IL-1F8, FIL-1h and IL-1H2, is a member of the IL-1 family of proteins. IL-36 beta is reported to be actively secreted. Cells expressing IL-36 beta include resting and activated monocytes, and B cells. The receptor for IL-36 beta is thought to be a combination of IL-1 Rrp2 and IL-1 RAcP. Recombinant IL-36 beta activates pathways involving NF-kB and MAPK in an IL-1 Rrp2-dependent manner.

Long Name

Interleukin 36 beta/Interleukin 1 Family 8

Alternate Names

FIL1 eta, IL-1 eta, IL-1H2, IL1F8, IL36 beta, IL36B

Gene Symbol

IL36B

UniProt

NP_775270

Additional IL-36 beta/IL-1F8 Products

Product Documents for IL-36 beta/IL-1F8 Antibody (1E4) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for IL-36 beta/IL-1F8 Antibody (1E4) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

IL-1 Family

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FAQs

No product specific FAQs exist for this product.

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Associated Pathways

IL-1 Family Signaling Pathways IL-1 Family Signaling Pathway Thumbnail