IL6R Antibody (AP-MAB0848) - Azide and BSA Free

Novus Biologicals | Catalog # NBP1-06670

Novus Biologicals
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Key Product Details

Species Reactivity

Mouse

Applications

Block/Neutralize, Flow Cytometry, Immunoprecipitation, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2 Clone # AP-MAB0848

Format

Azide and BSA Free
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Product Specifications

Immunogen

Mouse recombinant IL-6R alpha chain

Clonality

Monoclonal

Host

Rat

Isotype

IgG2

Applications for IL6R Antibody (AP-MAB0848) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunoprecipitation

1:10-1:500
Application Notes
Extremely low level of LPS (< 0.002EU/ug IgG). This antibody is CyTOF ready.

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Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: IL6R

CD126 is an 80 kD IL-6 receptor alpha chain also known as IL-6R. It is a member of the immunoglobulin superfamily that is expressed on activated T and B cells, monocytes, hepatocytes, and plasma cells. High affinity IL-6 receptors are formed by the non-covalent association of CD126 and the IL-6 receptor beta chain (CD130 or gp130). CD126 binds IL-6 with low affinity, but does not signal. The beta chain (gp130, CD130) does not bind IL-6 by itself, but associates with the alpha-chain/IL-6 complex to initiate signal transduction. IL-6 binding to the receptor complex results in the stimulation of B and T cells, and hematopoietic precursor stimulation and differentiation.

Alternate Names

CD126, CD126 antigen, gp80, IL-6 receptor subunit alpha, IL-6R 1, IL-6R subunit alpha, IL-6R-1, IL6RA, IL-6RA, IL-6R-alpha, interleukin 6 receptor, interleukin 6 receptor alpha subunit, interleukin-6 receptor subunit alpha, Membrane glycoprotein 80, MGC104991

Entrez Gene IDs

16194 (Mouse)

Gene Symbol

IL6RA

UniProt

Additional IL6R Products

Product Documents for IL6R Antibody (AP-MAB0848) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for IL6R Antibody (AP-MAB0848) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for IL6R Antibody (AP-MAB0848) - Azide and BSA Free

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  • Q: I am looking for an antibody to detect murine IL6 receptor and would like to use it to block the receptor in vivo in mouse. Your data sheet briefly states, that this antibody can be used for "Blocking/Neutralizing 50-200 molar excess" - but not more infor

    A: The blocking assay was performed using murine IL-6Ra over-expressing mouse fibroblasts. NBP1-06670 blocks IL-6 induced cell proliferation in a dose dependent manner, however no in vivo testing has been performed in house.

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