Indocyanine green

Tocris Bioscience | Catalog # 7510

Near-infrared (NIR) fluorescent dye; suitable for in vivo imaging
Tocris Bioscience

Key Product Details

Description

Near-infrared (NIR) fluorescent dye; suitable for in vivo imaging

Alternative Names

ICG

Wavelength

Excitation = 787 nm, Emission = 815 nm
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Product Description

Key information: Indocyanine green (ICG) is a near-infrared (NIR) fluorescent dye suitable for in vivo imaging.

Application: Fluorescence microscopy, in vivo imaging.

Properties and Photophysical Data: Indocyanine green is a NIR fluorescent dye operating in the 700-900 nm wavelength range. It binds to β-lipoproteins, particularly albumin, in plasma and exhibits fluorescence emission in the shortwave infrared window (SWIR; 1,000-2,000 nm) which enables in vivo imaging, including intravital microscopy, non-invasive real-time imaging in blood and lymph vessels, and imaging of hepatobiliary clearance. Imaging of indocyanine green in SWIR shows increased sensitivity, contrast, and resolution of fine anatomical structures over NIR. Excitation and emission maxima (λ) are 787 nm and 815 nm, respectively; quantum yield = 0.14; extinction coefficient = 223,000 M-1cm-1; A280 correction factor = 0.07.

This product is supplied with a high degree of hydration and may contain residual inorganic salts, the amount of which are batch dependent. Please refer to the Certificate of Analysis to obtain the batch specific Net Product Content.

Optical Data

Indocyanine green Dye Spectra
Emission Color Near-IR
Brightness
λabs 787 nm
λem 815 nm
Extinction Coefficient (ε) 223000 M-1cm-1
Quantum Yield (φ) 0.14
Closest Laser line 785 nm
pH Sensitivity No
Correction Factor 280 0.07
Application Fluorescence Microscopy, in vivo Imaging

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Product Specifications for Indocyanine green

Molecular Weight

774.97

Formula

C43H47N2O6S2Na

Storage

Store at -20°C

Purity

≥95% (HPLC)

Chemical Name

Sodium 4-(2-((1E,3E,5E,7Z)-7-(1,1-Dimethyl-3-(4-sulfonatobutyl)-1,3-dihydro-2H-benzo[e]indol-2-ylidene)hepta-1,3,5-trien-1-yl)-1,1-dimethyl-1H-benzo[e]indol-3-ium-3-yl)butane-1-sulfonate

CAS Number

3599-32-4

PubChem ID

45101199

InChI Key

MOFVSTNWEDAEEK-UHFFFAOYSA-M

SMILES

CC1(/C(N(C2=C1C3=CC=CC=C3C=C2)CCCCS(=O)(O[Na])=O)=C/C=C/C=C/C=C/C4=[N+](C5=C(C4(C)C)C6=CC=CC=C6C=C5)CCCCS([O-])(=O)=O)C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 38.75 50
DMSO 15.5 20

Preparing Stock Solutions for Indocyanine green

The following data is based on the product molecular weight 774.97.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.5 mM 2.58 mL 12.90 mL 25.81 mL
2.5 mM 0.52 mL 2.58 mL 5.16 mL
5 mM 0.26 mL 1.29 mL 2.58 mL
25 mM 0.05 mL 0.26 mL 0.52 mL

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Background References

References are publications that support the biological activity of the product.

Product Documents for Indocyanine green

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Indocyanine green

For research use only

Citations for Indocyanine green

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