Indocyanine green

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Indocyanine green | CAS No. 3599-32-4 | Fluorescent Dyes
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Description: Near-infrared (NIR) fluorescent dye; suitable for in vivo imaging
Alternative Names: ICG

Chemical Name: Sodium 4-(2-((1E,3E,5E,7Z)-7-(1,1-Dimethyl-3-(4-sulfonatobutyl)-1,3-dihydro-2H-benzo[e]indol-2-ylidene)hepta-1,3,5-trien-1-yl)-1,1-dimethyl-1H-benzo[e]indol-3-ium-3-yl)butane-1-sulfonate

Purity: ≥95%

Product Details

Biological Activity

Key information: Indocyanine green (ICG) is a near-infrared (NIR) fluorescent dye suitable for in vivo imaging.

Application: Fluorescence microscopy, in vivo imaging.

Properties and Photophysical Data: Indocyanine green is a NIR fluorescent dye operating in the 700-900 nm wavelength range. It binds to β-lipoproteins, particularly albumin, in plasma and exhibits fluorescence emission in the shortwave infrared window (SWIR; 1,000-2,000 nm) which enables in vivo imaging, including intravital microscopy, non-invasive real-time imaging in blood and lymph vessels, and imaging of hepatobiliary clearance. Imaging of indocyanine green in SWIR shows increased sensitivity, contrast, and resolution of fine anatomical structures over NIR. Excitation and emission maxima (λ) are 787 nm and 815 nm, respectively; quantum yield = 0.14; extinction coefficient = 223,000 M-1cm-1; A280 correction factor = 0.07.

This product is supplied with a high degree of hydration and may contain residual inorganic salts, the amount of which are batch dependent. Please refer to the Certificate of Analysis to obtain the batch specific Net Product Content.

Optical Data for Indocyanine green

Indocyanine green Dye Spectra
Emission Color Near-IR
λabs 787 nm
λem 815 nm
Extinction Coefficient (ε) 223000 M-1cm-1
Quantum Yield (φ) 0.14
Closest Laser line 785 nm
pH Sensitivity No
Correction Factor 280 0.07
Application Fluorescence Microscopy, in vivo Imaging

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Technical Data

Soluble to 20 mM in DMSO and to 50 mM in water
Store at -20°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

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