Integrin alpha 6/CD49f Antibody (SR45-00)
Novus Biologicals | Catalog # NBP2-67311
Recombinant Monoclonal Antibody.
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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # SR45-00 expressed in HEK293
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Product Specifications
Immunogen
Recombinant protein within human Integrin alpha 6/CD49f aa 1-100. (SwissProt: P23229 Human; SwissProt: Q61739 Mouse; SwissProt: Q63258 Rat)
Localization
Cell membrane.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit Integrin alpha 6/CD49f Antibody (SR45-00) (NBP2-67311) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Integrin alpha 6/CD49f Antibody (SR45-00)
Western Blot: Integrin alpha 6/CD49f Antibody (SR45-00) [NBP2-67311]
Western Blot: Integrin alpha 6/CD49f Antibody (SR45-00) [NBP2-67311] - Western blot analysis of Integrin alpha 6/CD49f on A431 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 houImmunohistochemistry-Paraffin: Integrin alpha 6/CD49f Antibody (SR45-00) [NBP2-67311]
Immunohistochemistry-Paraffin: Integrin alpha 6/CD49f Antibody (SR45-00) [NBP2-67311] - Analysis of paraffin-embedded mouse stomach tissue using anti-Integrin alpha 6 antibody. Counter stained with hematoxylin.Western Blot: Integrin alpha 6/CD49f Antibody (SR45-00) [NBP2-67311]
Western Blot: Integrin alpha 6/CD49f Antibody (SR45-00) [NBP2-67311] - Analysis of Integrin alpha 6 on Raji cell lysates using anti-Integrin alpha 6 antibody at 1/500 dilution.Immunohistochemistry-Paraffin: Integrin alpha 6/CD49f Antibody (SR45-00) [NBP2-67311]
Immunohistochemistry-Paraffin: Integrin alpha 6/CD49f Antibody (SR45-00) [NBP2-67311] - Analysis of paraffin-embedded human colon cancer tissue using anti-Integrin alpha 6 antibody. Counter stained with hematoxylin.Immunohistochemistry-Paraffin: Integrin alpha 6/CD49f Antibody (SR45-00) [NBP2-67311]
Immunohistochemistry-Paraffin: Integrin alpha 6/CD49f Antibody (SR45-00) [NBP2-67311] - Analysis of paraffin-embedded mouse colon tissue using anti-Integrin alpha 6 antibody. Counter stained with hematoxylin.Applications for Integrin alpha 6/CD49f Antibody (SR45-00)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:50-1:200
Western Blot
1:500-1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Integrin alpha 6/CD49f
Alternate Names
CD49f, ITGA6, Platelet gpl, VLA-6
Gene Symbol
ITGA6
Additional Integrin alpha 6/CD49f Products
Product Documents for Integrin alpha 6/CD49f Antibody (SR45-00)
Product Specific Notices for Integrin alpha 6/CD49f Antibody (SR45-00)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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