Integrin beta 2/CD18 Antibody (MEM-48) [FITC]

Name: Integrin beta 2/CD18 Antibody (MEM-48) [FITC]
Brand: Novus Biologicals
SKU: NB500-380
Price: 499 USD
Availability: InStock

Novus Biologicals | Catalog # NB500-380

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Product Specifications
Scientific Data
Applications
Spectra Viewer
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Canine (Negative)

Applications

Flow Cytometry

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Monoclonal Mouse IgG₁ Clone # MEM-48

View all Integrin beta 2/CD18 Primary Antibodies »

Product Specifications

Immunogen

Leukocytes of a patient suffering from a LGL-type leukemia.

Specificity

The antibody MEM-48 recognizes an epitope involving residues 534-546 in cysteine-rich repeat 3 of the CD18 antigen (integrin beta2 subunit; beta2 integrin). CD18 is a 90-95 kDa type I transmembrane protein expressed on all leukocytes.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Theoretical MW

95 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Integrin beta 2/CD18 Antibody (MEM-48) [FITC]

Flow Cytometry: Integrin beta 2/CD18 Antibody (MEM-48) [FITC] [NB500-380] - Surface staining of human peripheral blood with anti-CD18 (MEM-48) FITC.

Applications for Integrin beta 2/CD18 Antibody (MEM-48) [FITC]

Application

Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

The reagent is designed for Flow Cytometry analysis of human blood cells using 20 ul reagent / 100 ul of whole blood or 106 cells in a suspension.

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Size Exclusion Chromatography

Formulation

Phosphate buffered saline (PBS), pH 7.4

Preservative

15mM Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Integrin beta 2/CD18

CD18, integrin a2 subunit, forms heterodimers with four types of CD11 molecule to constitute leukocyte (a2) integrins: aLa2 (CD11a/CD18, LFA-1), aMa2 (CD11b/CD18, Mac-1, CR3), aXa2 (CD11c/CD18) and aDa2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1; severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.

Alternate Names

CD18, ITGB2, LCAMB, LFA-1, LFA-1 beta, MAC-1 beta, MF17, p150,95 beta

Gene Symbol

ITGB2

Additional Integrin beta 2/CD18 Products

Product Documents for Integrin beta 2/CD18 Antibody (MEM-48) [FITC]

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Certificate of Analysis

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Product Specific Notices for Integrin beta 2/CD18 Antibody (MEM-48) [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.