Involucrin Antibody (SPM259) [Allophycocyanin]

Novus Biologicals | Catalog # NBP2-34402APC

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Porcine, Canine, Monkey, Mouse (Negative), Primate

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # SPM259
View all Involucrin Primary Antibodies »

Product Specifications

Immunogen

Purified involucrin from human keratinocytes (Uniprot: P07476)

Epitope

aa 421-568

Reactivity Notes

Does not react with Mouse.

Localization

Cytoplasmic

Marker

Squamous Cell Terminal Differentiation Marker

Specificity

It recognizes a protein of 66kDa-170kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this monoclonal antibody reacts with a 120kDa protein. It stains the involucrin in a variety of sizes: 170kDa in MCF-7 cells, a doublet of ~115kDa and 150kDa in gorilla and owl monkey, 66kDa in dog, and a doublet of 105kDa in pig. Its epitope maps between codon 421-568 of human involucrin. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal; expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Scientific Data Images for Involucrin Antibody (SPM259) [Allophycocyanin]

Flow Cytometry: Involucrin Antibody (SPM259) [Allophycocyanin] [NBP2-34402APC] - Human keratinocytes. Image from verified customer review.

Applications for Involucrin Antibody (SPM259) [Allophycocyanin]

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NBP2-34402APC in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Involucrin

Stains paraffin embedded human tissue, so will be useful for studies of changes in involucrin expression in pathological studies.

Alternate Names

involucrin

Gene Symbol

IVL

Additional Involucrin Products

Product Documents for Involucrin Antibody (SPM259) [Allophycocyanin]

Certificate of Analysis

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Product Specific Notices for Involucrin Antibody (SPM259) [Allophycocyanin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Involucrin Antibody (SPM259) [Allophycocyanin] (1)

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  • Involucrin Antibody (SPM259) [Allophycocyanin]
    Name: Julien COUTIER
    Application: Flow Cytometry
    Sample Tested: Human keratinocytes
    Species: Human
    Verified Customer | Posted 08/23/2018
    Used at the dilution of 1/100e in washing buffer, after permeabilization with the cytofix/cytoperm kit (BD), 1H at 4°C.
    Involucrin Antibody (SPM259) [Allophycocyanin] NBP2-34402APC

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