ISCU Antibody (OTI4F5)

Novus Biologicals | Catalog # NBP2-45436

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI4F5
View all ISCU Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human ISCU (NP_055116) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

15.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ISCU Antibody (OTI4F5)

Western Blot: ISCU Antibody (OTI4F5) [NBP2-45436]

Western Blot: ISCU Antibody (OTI4F5) [NBP2-45436]

Western Blot: ISCU Antibody (4F5) [NBP2-45436] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ISCU.
Immunohistochemistry: ISCU Antibody (OTI4F5) [NBP2-45436]

Immunohistochemistry: ISCU Antibody (OTI4F5) [NBP2-45436]

Immunohistochemistry: ISCU Antibody (4F5) [NBP2-45436] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: ISCU Antibody (OTI4F5) [NBP2-45436]

Immunohistochemistry: ISCU Antibody (OTI4F5) [NBP2-45436]

Immunohistochemistry: ISCU Antibody (4F5) [NBP2-45436] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: ISCU Antibody (OTI4F5) [NBP2-45436]

Immunohistochemistry: ISCU Antibody (OTI4F5) [NBP2-45436]

Immunohistochemistry: ISCU Antibody (4F5) [NBP2-45436] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
ISCU Antibody (OTI4F5)

ISCU Antibody (OTI4F5)[NBP2-45436]

ISCU Antibody (OTI4F5)[NBP2-45436]Simple Western� analysis of endogenous protein KRAS from HELA lysates (0.5 mg/mL) using KRAS Mouse Monoclonal Antibody. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess� Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12�230 kDa Separation Module.

Applications for ISCU Antibody (OTI4F5)

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ISCU

Iron-sulfur (Fe-S) clusters are necessary for several mitochondrial enzymes and other subcellular compartment proteins. They contain sulfur and iron, and are created via several steps that include cysteine desulfurases, iron donors, chaperones, and scaffold proteins. This gene encodes the two isomeric forms, ISCU1 and ISCU2, of the Fe-S cluster scaffold protein. Mutations in this gene have been found in patients with myopathy with severe exercise intolerance and myoglobinuria.

Alternate Names

2310020H20Rik, HML, hnifU, iron-sulfur cluster assembly enzyme ISCU, mitochondrial, iron-sulfur cluster scaffold homolog (E. coli), IscU, IscU iron-sulfur cluster scaffold homolog, IscU iron-sulfur cluster scaffold homolog (E. coli), ISU2, MGC74517, NIFU, NifU-like N-terminal domain containing, NifU-like N-terminal domain-containing protein, NifU-like protein, NIFUN

Gene Symbol

ISCU

Additional ISCU Products

Product Documents for ISCU Antibody (OTI4F5)

Certificate of Analysis

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Product Specific Notices for ISCU Antibody (OTI4F5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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