IZUMO1 Recombinant Protein Antigen

Novus Biologicals | Catalog # NBP1-83086PEP

Novus Biologicals
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Key Product Details

Source

E. coli

Tag

N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

Applications

Antibody Competition
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Product Specifications

Description

A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human IZUMO1.

Source: E. coli

Amino Acid Sequence: VVLALKSLEKDYLPGHLDAKHHKAMMERVENAVKDFQELSLNEDAYMGVVDEATLQKGSWSLLKDLKRITDSDVKGDLFVKELFWMLHLQKETFATYVARFQKEAYCPNKCGVMLQTLIWCKNCKKEVHACRKSYDCGERNVEVPQM

Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

Purity

>80% by SDS-PAGE and Coomassie blue staining

Predicted Molecular Mass

35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Applications

Antibody Competition (10 - 100 molar excess)

Application Notes

This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP1-83086.

It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

Protein / Peptide Type

Recombinant Protein Antigen

Reviewed Applications

Read 1 review rated 5 using NBP1-83086PEP in the following applications:

Formulation, Preparation, and Storage

NBP1-83086PEP
Formulation PBS and 1M Urea, pH 7.4.
Preservative No Preservative
Concentration Please see the vial label for concentration. If unlisted please contact technical services.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20C. Avoid freeze-thaw cycles.

Background: IZUMO1

The sperm-specific protein Izumo, named for a Japanese shrine dedicated to marriage, is essential for sperm-egg plasmamembrane binding and fusion (Inoue et al., 2005 (PubMed 15759005)).(supplied by OMIM)

Long Name

Izumo Sperm-egg Fusion 1

Alternate Names

OBF

Gene Symbol

IZUMO1

Additional IZUMO1 Products

Product Documents for IZUMO1 Recombinant Protein Antigen

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for IZUMO1 Recombinant Protein Antigen

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Citations for IZUMO1 Recombinant Protein Antigen

Customer Reviews for IZUMO1 Recombinant Protein Antigen (1)

5 out of 5
1 Customer Rating
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4 Stars
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  • IZUMO1 Recombinant Protein Antigen
    Name: Anonymous
    Application: Blocking peptide in Western blot
    Verified Customer | Posted 07/11/2019
    IZUMO1 antibody together with the IZUMO1 recombinant protein antigen (IZUMO1 – blocking peptide) and its loading control ( alpha -tubulin). Lanes P: pig. Lanes H: human.
    Used as a blocking peptide for IZUMO1 antibody (NBP1-83086) in Western blot

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