Janelia Fluor® 525 ADA

Tocris Bioscience | Catalog # 8962

Yellow JF-based fluorescent dye for staining live Staphilococcus aureus bacteria
Tocris Bioscience
100% Guarantee

Key Product Details

Description

Yellow JF-based fluorescent dye for staining live Staphilococcus aureus bacteria

Wavelength

Excitation = 525 nm, Emission = 549 nm
View all Fluorescent Probes for Imaging Bacteria »

Product Description

Key information: Janelia Fluor® 525 ADA is a yellow fluorescent dye suitable for bacterial imaging applications, consisting of a bright Janelia Fluor® fluorophore bearing the unnatural aminoacid D-Alanine. Incorporation of fluorescent D-aminoacids in gram negative and positive bacteria can selectively label bacterial membranes, resulting in bright fluorescence around the bacteria and minimal or no signal detected inside.

Used for: selectively staining Staphylococcus aureus.

Application: confocal microscopy and flow cytometry.

Properties and Photophysical Data: Excitation and emission maxima (λ) are 525 nm and 549 nm, respectively; quantum yield = 0.91; extinction coefficient = 122,000 M-1cm-1; lactone-zwitterion equilibrium constant (KL-Z) = 0.068.

Licensing Information

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Optical Data

Emission Color Yellow
λabs 525 nm
λem 549 nm
Extinction Coefficient (ε) 122,000 M-1cm-1
Quantum Yield (φ) 0.91
Correction Factor 280 0.185

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Product Specifications for Janelia Fluor® 525 ADA

Formula

C30H24F4N4O6.2CF3CO2H

Storage

Store at -20°C

Purity

≥95% (HPLC)

Chemical Name

(R)-4-((2-Amino-2-carboxyethyl)carbamoyl)-2-(3-(3,3-difluoroazetidin-1-ium-1-ylidene)-6-(3,3-difluoroazetidin-1-yl)-3H-xanthen-9-yl)benzoate ditrifluoroacetate

InChI Key

RRHAUQCSJYRQEK-GJICFQLNSA-N

SMILES

O=C(NC[C@@H](N)C(O)=O)C1=CC=C(C([O-])=O)C(C(C2=C(O3)C=C(N4CC(F)(F)C4)C=C2)=C5C3=C/C(C=C5)=[N+]6CC(F)(F)C\6)=C1.OC(C(F)(F)F)=O.OC(C(F)(F)F)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solubility Soluble to 8.41mg/ml in DMSO

Calculators

Calculate the dilution required to prepare a stock solution.

x
=
x

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background References

References are publications that support the biological activity of the product.

There are currently no references for this product.

Product Documents for Janelia Fluor® 525 ADA

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Janelia Fluor® 525 ADA

For research use only

Customer Reviews for Janelia Fluor® 525 ADA

There are currently no reviews for this product. Be the first to review Janelia Fluor® 525 ADA and earn rewards!

Have you used Janelia Fluor® 525 ADA?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card