Janelia Fluor® 525, BromoCatch™ Ligand

Tocris Bioscience | Catalog # 8997

Fluorogenic Janelia Fluor® 525 conjugated covalent ligand for BromoCatch™, enabling no-wash live-cell imaging
Tocris Bioscience

Key Product Details

Description

Fluorogenic Janelia Fluor® 525 conjugated covalent ligand for BromoCatch™, enabling no-wash live-cell imaging

Wavelength

Excitation = 534 nm, Emission = 557 nm
View all BromoCatch Platform »

Product Description

Janelia Fluor® 525, BromoCatch™ Ligand is a fluorogenic BromoCatch™ ligand functionalized with Janelia Fluor® 525, designed for real-time, no-wash imaging of BromoCatch-tagged proteins in live cells. Upon covalent binding to the BromoCatch™ tag, this probe undergoes a fluorescence switch-on due to the environmental sensitivity of the Janelia Fluor® 525 dye, delivering bright signal with minimal background fluorescence. Compatible with super-resolution imaging and suitable for dynamic cellular studies, Janelia Fluor® 525, BromoCatch™ Ligand offers a powerful tool for live-cell tracking of BromoCatch-tagged proteins with excellent photostability and imaging contrast.

Please refer to this protocol for further information on how to use this product.

Janelia Fluor® is a registered trademark of Howard Hughes Medical Institute.

This product is part of an early adopter program.

BromoCatch™ is a trademark of Bio-Techne Corp.

Licensing Information

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus and the University of Dundee

Optical Data

Janelia Fluor<sup>®</sup> 525, BromoCatch™ Ligand Dye Spectra
λabs 534 nm
λem 557 nm

Spectra Viewer

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Product Specifications for Janelia Fluor® 525, BromoCatch™ Ligand

Molecular Weight

1146.23

Formula

C59H59F4N9O9S

Storage

Store at -20°C

Purity

≥90% (HPLC)

Chemical Name

4-(((R)-14-((S)-4-(4-Acrylamidophenyl)-2,3,9-trimethyl-6H-thieno[3,2-f][1,2,4]triazolo[4,3-a][1,4]diazepin-6-yl)-13-oxo-3,6,9-trioxa-12-azahexadecyl)carbamoyl)-2-(3-(3,3-difluoroazetidin-1-ium-1-ylidene)-6-(3,3-difluoroazetidin-1-yl)-3H-xanthen-9-yl)benzoate

InChI Key

CIYUOPFNGGEXOY-RWZZRXGDSA-N

SMILES

CC1=C(C)SC2=C1C(C3=CC=C(C=C3)NC(C=C)=O)=N[C@@H]([C@H](C(NCCOCCOCCOCCNC(C4=CC=C(C(C5=C6C(OC7=CC(N8CC(F)(F)C8)=CC=C57)=C/C(C=C6)=[N+]9CC(F)(F)C/9)=C4)C([O-])=O)=O)=O)CC)C%10=NN=C(C)N2%10

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

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Background References

References are publications that support the biological activity of the product.

Product Documents for Janelia Fluor® 525, BromoCatch™ Ligand

Certificate of Analysis

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Product Specific Notices for Janelia Fluor® 525, BromoCatch™ Ligand

For research use only

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