Janelia Fluor® 549, Azide
Tocris Bioscience | Catalog # 6501
Key Product Details
Description
Wavelength
Product Description
Janelia Fluor® 549, Azide is a fluorescent yellow dye; supplied as an azide for click chemisty. Suitable for confocal fluorescent imaging and super resolution microscopy (SRM) techniques, such as dSTORM (live and fixed cells) and STED. Also suitable for flow cytometry. Cell permeable. Excitation maximum = 549 nm; emission maximum = 571 nm; Quantum yield = 0.88; Extinction coefficient = 101,000 M-1cm-1; A280 correction factor is 0.169.Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research CampusOptical Data
| Emission Color | Yellow |
| Brightness | |
| λabs | 549 nm |
| λem | 571 nm |
| Extinction Coefficient (ε) | 101,000 M-1cm-1 |
| Quantum Yield (φ) | 0.88 |
| Closest Laser line | 532 nm |
| Reactive Group | Azide |
| Reactivity | Click |
| Cell Permeable | Yes |
| Correction Factor 280 | 0.169 |
| Application | Confocal imaging, dSTORM, STED, Flow |
| Alternative For | Alexa Fluor® 546, Alexa Fluor® 555, BDY TMR-X, Atto 550, CF 555, TAMRA, Cyanine 3 |
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Product Specifications for Janelia Fluor® 549, Azide
Molecular Weight
Formula
Storage
Purity
Chemical Name
PubChem ID
InChI Key
SMILES
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 65.47 | 100 |
Preparing Stock Solutions for Janelia Fluor® 549, Azide
The following data is based on the product molecular weight 654.71.
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.53 mL | 7.64 mL | 15.27 mL |
| 5 mM | 0.31 mL | 1.53 mL | 3.05 mL |
| 10 mM | 0.15 mL | 0.76 mL | 1.53 mL |
| 50 mM | 0.03 mL | 0.15 mL | 0.31 mL |
Calculators
Background References
References are publications that support the biological activity of the product.
- Grimm A general method to improve fluorophores for live-cell and single-molecule microscopy. Nat.Methods 2015 PMID: 25599551
Product Documents for Janelia Fluor® 549, Azide
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Janelia Fluor® 549, Azide
For research use only
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