Janelia Fluor® 549, Azide
Chemical Name: 3,6-Di-1-azetidinyl-9-[5-[[2-[2-[2-[2-azidoethoxy]ethoxy]ethoxy]ethyl]carbamoyl]-2-carboxyphenyl]xanthylium, inner salt
Biological ActivityFluorescent dye; supplied as an azide for click chemisty. Suitable for confocal fluorescent imaging and super resolution microscopy (SRM) techniques, such as dSTORM (live and fixed cells) and STED. Cell permeable. Excitation maximum = 549 nm; emission maximum = 571 nm; Quantum yield = 0.88; Extinction coefficient = 101,000 M-1cm-1; A280 correction factor is 0.169.
Janelia Fluor® 549, Azide Dye Spectra
|Extinction coefficient (ε)||101,000 M-1cm-1|
|Quantum yield (φ)||0.88|
|Closest Laser line||532 nm|
|Alternative for||Alexa Fluor® 546, Alexa Fluor® 555, Atto 550, BDY TMR-X, CF555, TAMRA, Cyanine 3|
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
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A general method to improve fluorophores for live-cell and single-molecule microscopy.
Grimm et al.
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