Janelia Fluor® 549, free acid
Chemical Name: 3,6-Di-1-azetidinyl-9-(2,5-dicarboxyphenyl)xanthylium, inner salt trifluoroacetate
Biological ActivityYellow fluorescent dye; supplied as a free acid. Used in protocol (2017 Grimm et al - see references below) for the synthesis of Janelia Fluor® HaloTag® and SNAP-Tag® ligands, for use in live cell imaging experiments. Excitation maximum = 549 nm; emission maximum = 571 nm; Quantum yield = 0.88; Extinction coefficient = 101,000 M-1cm-1.
Janelia Fluor® 549, free acid Dye Spectra
|Extinction coefficient (ε)||101,000 M-1cm-1|
|Quantum yield (φ)||0.88|
|Closest Laser line||532/561 nm|
|Alternative for||Alexa Fluor® 546, Alexa Fluor® 555, Atto 550, BDY TMR-X, CF555, TAMRA, Cyanine 3|
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
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For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
A general method to improve fluorophores for live-cell and single-molecule microscopy.
Grimm et al.
Synthesis of Janelia Fluor HaloTag and SNAP-Tag Ligands and Their Use in Cellular Imaging Experiments.
Grimm et al.
Methods Mol.Biol., 2017;1663:179
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