Janelia Fluor® 549, free acid

Tocris Bioscience | Catalog # 6503

Yellow dye (Reactive group: free acid). Application: flow cytometry, confocal microscopy, super resolution microscopy (SRM) including dSTORM & STED. Suitable for live cell imaging
Tocris Bioscience

Key Product Details

Description

Yellow dye (Reactive group: free acid). Application: flow cytometry, confocal microscopy, super resolution microscopy (SRM) including dSTORM & STED. Suitable for live cell imaging

Wavelength

Excitation = 549 nm, Emission = 571 nm
View all Free Acid Janelia Fluor Dyes »

Product Description

Key Information: Janelia Fluor® 549, free acid is a yellow fluorescent dye; supplied with a free acid reactive group. Suitable for live cell imaging.

Application: Suitable for flow cytometry, confocal microscopy, super resolution microscopy (SRM) including dSTORM (in both live and fixed cells) and STED. Used in protocol (2017 Grimm et al - see references below) for the synthesis of Janelia Fluor® HaloTag® and SNAP-tag® ligands. Cell permeable.

Properties and Photophysical Data: Excitation and emission maxima (λ) are 549 nm and 571 nm, respectively; quantum yield = 0.88; extinction coefficient = 101,000 M-1cm-1, A280 correction factor = 0.169; lactone-zwitterion equilibrium constant (KL-Z) = 3.5.

Janelia Fluor® is a registered trademark of Howard Hughes Medical Institute.

HaloTag® is a registered trademark of Promega Corporation, and SNAP-tag® is a registered trademark of New England BioLabs, Inc.

Licensing Information

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Optical Data

Janelia Fluor<sup>®</sup> 549, free acid Dye Spectra
Emission Color Yellow
Brightness
λabs 549 nm
λem 571 nm
Extinction Coefficient (ε) 101,000 M-1cm-1
Quantum Yield (φ) 0.88
Closest Laser line 532 nm
Reactive Group Free acid
Cell Permeable Yes
Correction Factor 280 0.169
Application Confocal Microscopy, dSTORM, STED, Flow Cytometry, Live-cell Imaging
Alternative For Alexa Fluor® 546, Alexa Fluor® 555, BDY TMR-X, Atto 550, CF 555, TAMRA, Cyanine 3

Trademarks and registered trademarks are the property of their respective owners.

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Product Specifications for Janelia Fluor® 549, free acid

Molecular Weight

568.50

Formula

C27H22N2O5.C2HF3O2

Storage

Store at -20°C

Purity

≥90% (HPLC)

Chemical Name

3,6-Di-1-azetidinyl-9-(2,5-dicarboxyphenyl)xanthylium, inner salt trifluoroacetate

CAS Number

2245946-45-4

PubChem ID

137919862

InChI Key

GFUAWSMWTYTASE-UHFFFAOYSA-N

SMILES

OC(C1=CC=C(C(C(C2=CC=C(C=C2OC3=C/4)N5CCC5)=C3C=CC4=[N+]6CCC/6)=C1)C([O-])=O)=O.FC(F)(F)C(O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 45.45 100

Preparing Stock Solutions for Janelia Fluor® 549, free acid

The following data is based on the product molecular weight 568.50.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.76 mL 8.80 mL 17.59 mL
5 mM 0.35 mL 1.76 mL 3.52 mL
10 mM 0.18 mL 0.88 mL 1.76 mL
50 mM 0.04 mL 0.18 mL 0.35 mL

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Background References

References are publications that support the biological activity of the product.

Product Documents for Janelia Fluor® 549, free acid

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Janelia Fluor® 549, free acid

For research use only

Citations for Janelia Fluor® 549, free acid

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