Janelia Fluor® 549, free acid

Catalog # Availability Size / Price Qty
6503/5
Janelia Fluor Dyes Small Molecule
1 Image
Description: Yellow fluorescent dye; supplied as a free acid

Chemical Name: 3,6-Di-1-azetidinyl-9-(2,5-dicarboxyphenyl)xanthylium, inner salt trifluoroacetate

Purity: ≥95%

Product Details
Reviews

Biological Activity

Yellow fluorescent dye; supplied as a free acid. Used in protocol (2017 Grimm et al - see references below) for the synthesis of Janelia Fluor® HaloTag® and SNAP-Tag® ligands, for use in live cell imaging experiments. Excitation maximum = 549 nm; emission maximum = 571 nm; Quantum yield = 0.88; Extinction coefficient = 101,000 M-1cm-1.

Janelia Fluor® 549, free acid Dye Spectra

Spectra charts
Emission colorYellow
Brightness
λabs549 nm
λem571 nm
Extinction coefficient (ε)101,000 M-1cm-1
Quantum yield (φ)0.88
Closest Laser line532/561 nm
Alternative forAlexa Fluor® 546, Alexa Fluor® 555, Atto 550, BDY TMR-X, CF555, TAMRA, Cyanine 3

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.


Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Technical Data

M.Wt:
568.5
Formula:
C27H22N2O5.C2HF3O2
Solubility:
Soluble to 100 mM in DMSO
Purity:
≥95%
Storage:
Store at -20°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Additional Information

Licensing Caveats:
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Product Datasheets

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