Janelia Fluor® 549, Maleimide
Tocris Bioscience | Catalog # 6500
Key Product Details
Description
Wavelength
Product Description
Key information:Janelia Fluor® 549, Maleimide is a yellow fluorescent dye, supplied with a maleimide reactive group for conjugation (thiol reactivity). Suitable for live cell imaging.
Application: Suitable for flow cytometry, confocal microscopy, super resolution microscopy (SRM) including dSTORM (in both live and fixed cells) and STED. Cell permeable.
Properties and Photophysical Data: Excitation and emission maxima (λ) are 549 nm and 571 nm, respectively; quantum yield = 0.88; extinction coefficient = 101,000 M-1cm-1; A280 correction factor = 0.169; lactone-zwitterion equilibrium constant (KL-Z) = 3.5.
Please see the product protocol for further information and a guide to protein/antibody labeling.
Janelia Fluor® is a registered trademark of Howard Hughes Medical Institute.
Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research CampusOptical Data
| Emission Color | Yellow |
| Brightness | |
| λabs | 549 nm |
| λem | 571 nm |
| Extinction Coefficient (ε) | 101,000 M-1cm-1 |
| Quantum Yield (φ) | 0.88 |
| Closest Laser line | 532 nm |
| Reactive Group | Maleimide |
| Reactivity | Thiols |
| Cell Permeable | Yes |
| Correction Factor 280 | 0.169 |
| Application | Confocal Imaging, dSTORM, STED, Flow Cytometry |
| Alternative For | Alexa Fluor® 546, Alexa Fluor® 555, BDY TMR-X, Atto 550, CF 555, TAMRA, Cyanine 3 |
Trademarks and registered trademarks are the property of their respective owners.
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Product Specifications for Janelia Fluor® 549, Maleimide
Molecular Weight
Formula
Storage
Purity
Chemical Name
InChI Key
SMILES
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 69.06 | 100 |
Preparing Stock Solutions for Janelia Fluor® 549, Maleimide
The following data is based on the product molecular weight 690.63.
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.45 mL | 7.24 mL | 14.48 mL |
| 5 mM | 0.29 mL | 1.45 mL | 2.90 mL |
| 10 mM | 0.14 mL | 0.72 mL | 1.45 mL |
| 50 mM | 0.03 mL | 0.14 mL | 0.29 mL |
Calculators
Background References
References are publications that support the biological activity of the product. See our Citations tab to view 6 publications citing the usage of this product.
- Zheng Rational design of fluorogenic and spontaneously blinking labels for super-resolution imaging. ACS Cent.Sci. 2019 PMID: 31572787
- Grimm A general method to improve fluorophores for live-cell and single-molecule microscopy. Nat.Methods. 2015 PMID: 25599551
Product Documents for Janelia Fluor® 549, Maleimide
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Janelia Fluor® 549, Maleimide
For research use only
Citations for Janelia Fluor® 549, Maleimide
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