Janelia Fluor® 549, Maleimide
Chemical Name: 2-(3-(Azetidin-1-ium-1-ylidene)-6-(azetidin-1-yl)-3H-xanthen-9-yl)-4-((2-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)ethyl)carbamoyl)benzoate trifluoroacetate
Biological ActivityJanelia Fluor® 549, Maleimide is a yellow fluorescent dye, supplied with a maleimide reactive group for conjugation (thiol reactivity). Suitable for confocal fluorescent imaging and super resolution microscopy (SRM) techniques, such as dSTORM (live and fixed cells) and STED. Also suitable for flow cytometry. Cell permeable. Excitation maximum = 549 nm; emission maximum = 571 nm; Quantum yield = 0.88; Extinction coefficient = 101,000 M-1cm-1; A280 correction factor is 0.169.
Please see the product protocol for further information and a guide to protein/antibody labeling.
Optical Data for Janelia Fluor® 549, Maleimide
|Extinction Coefficient (ε)||101,000 M-1cm-1|
|Quantum Yield (φ)||0.88|
|Closest Laser line||532 nm|
|Correction Factor 280||0.169|
|Application||Confocal imaging, dSTORM, STED, Flow|
|Alternative For||Alexa Fluor® 546, Alexa Fluor® 555, BDY TMR-X, Atto 550, CF 555, TAMRA, Cyanine 3|
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
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The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
A general method to improve fluorophores for live-cell and single-molecule microscopy
Grimm et al.
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