Janelia Fluor® 549, Maleimide

Catalog # Availability Size / Price Qty
6500/2
Janelia Fluor Dyes Small Molecule
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Description: Cell permeable yellow fluorescent dye; supplied with a maleimide reactive group

Chemical Name: 2-(3-(Azetidin-1-ium-1-ylidene)-6-(azetidin-1-yl)-3H-xanthen-9-yl)-4-((2-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)ethyl)carbamoyl)benzoate trifluoroacetate

Purity: ≥95%

Product Details
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Biological Activity

Yellow fluorescent dye, supplied with a maleimide reactive group for conjugation (thiol reactivity). Suitable for confocal fluorescent imaging and super resolution microscopy (SRM) techniques, such as dSTORM (live and fixed cells) and STED. Cell permeable. Excitation maximum = 549 nm; emission maximum = 571 nm; Quantum yield = 0.88; Extinction coefficient = 101,000 M-1cm-1; A280 correction factor is 0.169.

Optical Data for Janelia Fluor® 549, Maleimide

Janelia Fluor<sup>®</sup> 549, Maleimide Dye Spectra
Emission Color Yellow
Brightness
λabs 549 nm
λem 571 nm
Extinction Coefficient (ε) 101,000 M-1cm-1
Quantum Yield (φ) 0.88
Closest Laser line 532 nm
Reactive Group Maleimide
Reactivity Thiols
Cell Permeable Yes
Correction Factor 280 0.169
Application Confocal imaging, dSTORM, STED, Flow
Alternative For Alexa Fluor® 546, Alexa Fluor® 555, BDY TMR-X, Atto 550, CF 555, TAMRA, Cyanine 3

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.

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Technical Data

M.Wt:
690.63
Formula:
C33H28N4O6.C2HF3O2
Solubility:
Soluble to 100 mM in DMSO
Purity:
≥95%
Storage:
Store at -20°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Additional Information

Licensing Caveats:
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Background References

  1. A general method to improve fluorophores for live-cell and single-molecule microscopy
    Grimm et al.
    Nat.Methods., 2015;12:244

Product Datasheets

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