Janelia Fluor® 549, Tetrazine
Chemical Name: 3,6-Di-1-azetidinyl-9-[[4-[(1,2,4,5-tetrazin-3-yl)benzyl]carbamoyl]-2-carboxyphenyl]xanthylium, inner salt
Biological ActivityJanelia Fluor® 549, Tetrazine is a yellow fluorescent dye; supplied with a tetrazine reactive handle for copper-free click chemistry. Janelia Fluor® 549 can be coupled directly to the protein of interest with labeling unnatural amino acids by biorthogonal click chemistry in living primary neurons. Suitable for confocal fluorescent imaging and super resolution microscopy (SRM) techniques, such as dSTORM (live and fixed cells) and STED. Cell permeable. Suitable for live-cell imaging. Excitation maximum = 549 nm; emission maximum = 571 nm; Quantum yield = 0.88; Extinction coefficient = 101,000 M-1cm-1; A280 correction factor is 0.169.
Optical Data for Janelia Fluor® 549, Tetrazine
|Extinction Coefficient (ε)||101,000 M-1cm-1|
|Quantum Yield (φ)||0.88|
|Closest Laser line||532 nm|
|Correction Factor 280||0.169|
|Application||Confocal imaging, dSTORM, STED|
|Alternative For||Alexa Fluor® 546, Alexa Fluor® 555, BDY TMR-X, Atto 550, CF 555, TAMRA, Cyanine 3|
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
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For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Citation for Janelia Fluor® 549, Tetrazine
The citations listed below are publications that use Tocris products. Selected citations for Janelia Fluor® 549, Tetrazine include:
1 Citation: Showing 1 - 1
Minimal genetically encoded tags for fluorescent protein labeling in living neurons.
Authors: Arsic Et al.
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