Janelia Fluor® 549, Tetrazine

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Janelia Fluor® 549, Tetrazine | Click-Reactive Janelia Fluor Dyes
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Description: Yellow dye (Reactive group: tetrazine). Applications: super resolution microscopy e.g. dSTORM & STED, confocal and live cell imaging

Chemical Name: 3,6-Di-1-azetidinyl-9-[[4-[(1,2,4,5-tetrazin-3-yl)benzyl]carbamoyl]-2-carboxyphenyl]xanthylium, inner salt

Product Details
Citations (1)

Biological Activity

Janelia Fluor® 549, Tetrazine is a yellow fluorescent dye; supplied with a tetrazine reactive handle for copper-free click chemistry. Janelia Fluor® 549 can be coupled directly to the protein of interest with labeling unnatural amino acids by biorthogonal click chemistry in living primary neurons. Suitable for confocal fluorescent imaging and super resolution microscopy (SRM) techniques, such as dSTORM (live and fixed cells) and STED. Cell permeable. Suitable for live-cell imaging. Excitation maximum = 549 nm; emission maximum = 571 nm; Quantum yield = 0.88; Extinction coefficient = 101,000 M-1cm-1; A280 correction factor is 0.169.

Optical Data for Janelia Fluor® 549, Tetrazine

Janelia Fluor<sup>®</sup> 549, Tetrazine Dye Spectra
Emission Color Yellow
λabs 549 nm
λem 571 nm
Extinction Coefficient (ε) 101,000 M-1cm-1
Quantum Yield (φ) 0.88
Closest Laser line 532 nm
Reactive Group Tetrazine
Reactivity Copper-free click
Cell Permeable Yes
Correction Factor 280 0.169
Application Confocal imaging, dSTORM, STED
Alternative For Alexa Fluor® 546, Alexa Fluor® 555, BDY TMR-X, Atto 550, CF 555, TAMRA, Cyanine 3

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.

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Technical Data

Soluble to 10 mM in DMSO
Store at -20°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Additional Information

Licensing Caveats:
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Product Datasheets

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Citation for Janelia Fluor® 549, Tetrazine

The citations listed below are publications that use Tocris products. Selected citations for Janelia Fluor® 549, Tetrazine include:

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